N-(Propargyl-PEG2)-DBCO-PEG3-N-Boc enables formation of triazole linkage with azide-bearing compound via copper catalyzed Click Chemistry. Under mild acidic conditions, t-Boc group can be removed to yield the free amine. The propargyl group can be linked to azide-containing biomolecules via Click Chemistry. Reagent grade, for research purpose.
N-(Propargyl-PEG2)-DBCO-PEG3-N-Boc enables formation of triazole linkage with azide-bearing compound via copper catalyzed Click Chemistry. Under mild acidic conditions, t-Boc group can be removed to yield the free amine. The propargyl group can be linked to azide-containing biomolecules via Click Chemistry. Reagent grade, for research purpose.
Propargyl-PEG4-NHS ester is an amine reactive PEG linker with an alkyne group. Under the copper catalyzation, alkyne group can react with azide-bearing biomolecules to form a stable triazole linkage. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG4-NHS ester is an amine reactive PEG linker with an alkyne group. Under the copper catalyzation, alkyne group can react with azide-bearing biomolecules to form a stable triazole linkage. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
K-HDBA
SKU: 700004302
60 assays (manual) / 600 assays (microplate) / 740 assays (auto-analyser)
| Content: | 60 assays (manual) / 600 assays (microplate) / 740 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | D-3-Hydroxybutyric Acid |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 492 |
| Signal Response: | Increase |
| Linear Range: | 0.4 to 12 µg of D-3-hydroxybutyric acid per assay |
| Limit of Detection: | 0.074 mg/L |
| Reaction Time (min): | ~ 6 min |
| Application examples: | Egg, egg products (e.g. egg powder) and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Methods based on this principle have been accepted by EEC |
The D-3-Hydroxybutyric Acid (β-Hydroxybutyrate) Assay Kit is suitable for the specific measurement and analysis of D-hydroxybutyric acid in eggs and egg products and other foods and beverages.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Explore more organic acid assay kit products.
Advantages
The D-3-Hydroxybutyric Acid (β-Hydroxybutyrate) Assay Kit is suitable for the specific measurement and analysis of D-hydroxybutyric acid in eggs and egg products and other foods and beverages.
The Kit provides fast purification of high-quality DNA, RNA and Prote from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the HiPure Total RNA Purification System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Specifications
| Features | Specifications |
| Main Functions | Co-isolation DNA and RNA /protein from a single sample (cells, soft tissue, plant sample) |
| Applications | SDS-PAGE electrophoresis and western blot, etc |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Culture cells, animal tissues, plant fungi, yeast, bacteria and other samples |
| Sample amount | Cultured cells: < 10^7Animal tissue: ≤ 20 mgPlant samples: ≤ 150 mgYeast cells: 2 x 10^6 – 5 x 10^7 |
The Kit isolates total RNA from up to 10 7 cells or 30 mg tissue. A short workflow enables RNAisolation with genomic DNA removal in less than 25 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30 µl water using the Kit.
Advantages
Kit Contents
| Contents | R521102 | R521103 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure DNA Mini Columns | 50 | 250 |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml Collection Tubes | 100 | 2 x 250 |
| Buffer RLC | 50 ml | 200 ml |
| Buffer GW1* | 22 ml | 66 ml |
| Buffer RW1 | 50 ml | 200 ml |
| Buffer RW2* | 50 ml | 3 x 50 ml |
| RNase Free Water | 10 ml | 30 ml |
| Elution Buffer | 10 ml | 30 ml |
| Buffer ALO(5%SDS) | 10 ml | 30 ml |
Storage and Stability
HiPure Kit can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. During shipment, crystals or precipitation may form in the Buffer RLC. Dissolve by warming buffer to 37°C.
The Kit provides fast purification of high-quality DNA, RNA and Prote from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the HiPure Total RNA Purification System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
