N-(Propargyl-PEG2)-N-bis(PEG3-t-butyl ester) is a 3-arm PEG reagent with a propargyl groups and two t-butyl ester groups. The propargyl group can react with azides via copper catalyzed Click Chemistry reaction to yield a stable triazole linkage. The t-butyl ester groups can be deprotected under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
N-(Propargyl-PEG2)-N-bis(PEG3-t-butyl ester) is a 3-arm PEG reagent with a propargyl groups and two t-butyl ester groups. The propargyl group can react with azides via copper catalyzed Click Chemistry reaction to yield a stable triazole linkage. The t-butyl ester groups can be deprotected under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
50 & 150 reactions
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and pyrosequencing.
Specifications
| Features | Specifications |
| Main Functions | Co-isolation DNA and RNA from FFPE tissue |
| Applications | RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc. |
| Purification method | Polydisperse silicon based magnetic beads (DNA)Monodisperse carbonyl magnetic beads (RNA) |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Adaptive instrument | Nucleic acid extractor and pipetting workstation |
| Sample type | FFPE slice, FFPE puncture sample, embedded tissue |
| Sample amount | No more than six 10 µm sections of 150 mm2 surface area or three 20µm sections of 150 mm2 surface area |
| Yield | DNA: 1 – 10 μg, RNA: 1 – 25 μg |
The sample is lysed and digested under the action of lysate and protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, the supernatant contain RNA was collected and bind to MagBind Particles. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Elution Buffer.
Advantages
Kit Contents
| Contents | R632701 | R632702 | R632703 |
| Purification Times | 48 Preps | 96 Preps | 5 x 96 Preps |
| MagBind Particles | 1.1 ml | 2.5 ml | 11 ml |
| MagPure Particles N | 1.1 ml | 2.5 ml | 11 ml |
| Proteinase K | 24 mg | 48 mg | 220 mg |
| Protease Dissolve Buffer | 3 ml | 10 ml | 15 ml |
| Buffer DPS | 50 ml | 100 ml | 2 x 250 ml |
| Buffer ATL | 20 ml | 30 ml | 120 ml |
| Buffer BST1 | 20 ml | 40 ml | 200 ml |
| Buffer BXW1* | 44 ml | 110 ml | 3 x 110 ml |
| RNase Free Water | 15 ml | 30 ml | 120 ml |
Storage and Stability
Proteinase K, MagPure Particles N and MagBind Particles should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stablefor at least 18 months under these conditions.
Experiment Data
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and pyrosequencing.
The Respiratory Syncytial Virus Rapid Antigen Assay is a lateral flow chromatographic immunoassay for the qualitative detection of RSV. It is suitable for the auxiliary detection of Respiratory Syncytial virus infection.
Format: 25 tests per kit
Swab Collection Method: Nasopharyngeal, Anterior Nasal, Oropharyngeal
Results: 20 minutes
