N-(Propargyl-PEG4)-N-bis(PEG4-acid) is a branched crosslinking reagent with a propargyl group and two terminal carboxylic acids. The propargyl group can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acids can react with primary amino groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
N-(Propargyl-PEG4)-N-bis(PEG4-acid) is a branched crosslinking reagent with a propargyl group and two terminal carboxylic acids. The propargyl group can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acids can react with primary amino groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This test discovers beer germs in a timely and precise manner
Method/Platform
PCR
Range/Assay Sensivity
10^4 – 10^5 cfu/mL
Test Principle
The technological basis for the GenLine tests is the polymerase chain reaction (PCR) combined with lateral flow tests.
Labelled specific primers are used to amplify specific DNA fragments. In addition to the target gene, a control gene, which is also present in the PCR mixes, is amplified in order to make sure that the PCR process works properly.
The resulting PCR products carry the labels of the incorporated primers.
In a second part of the test, the created PCR products are detected by a lateral flow Test Strip. A “molecular sandwich” is formed and becomes visible as a line on the test Strip.
Document
Brief Instructions The PCR reagents and the samples are prepared. After the addition of the sample to the PCR reagents, the PCR is started. The resulting PCR products are detected by a simple lateral flow test
Principle: Peptone provide nitrogen, vitamins and growth factors; sodium chloride to maintain osmotic equilibrium; lactose into fermentable sugars.
Formulation（per liter）:
Pancreatic Digest of Gelatin
20 g
Lactose Monohydrate
10g
Dehydrated Ox Bile
5g
Bromocresol Purple
10mg
final pH
7.3±0.2
How to use： 1.Suspend 35g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 ℃ autoclave for 15min. 2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
