N-t-Boc-Aminooxy-PEG4-N-(PEG2-Propargyl) is a click chemistry crosslinker. The propargyl group is reactive with azide-containing compounds or biomolecules through copper catalyzed Click Chemistry to yield a stable triazole linkage. t-Boc-aminooxy can be deprotected under mild acidic conditions. The hydrophilic PEG linker improves solubility in aqueous media.
N-t-Boc-Aminooxy-PEG4-N-(PEG2-Propargyl) is a click chemistry crosslinker. The propargyl group is reactive with azide-containing compounds or biomolecules through copper catalyzed Click Chemistry to yield a stable triazole linkage. t-Boc-aminooxy can be deprotected under mild acidic conditions. The hydrophilic PEG linker improves solubility in aqueous media.
ProbeSure Master Mix combines class-leading performance with the most competitive prices in the market. ProbeSure Master Mix has provided users with accurate, robust, and consistent performance for many years. It has been carefully optimised to work over a wide range of reaction volumes and sample template qualities.
ProbeSure Master Mix is supplied at 2x concentration for convenience and is supplied with the ROX normalising dye at either high level (500 nM final concentration), low level (25 nM final concentration) or without ROX.
This kit purifies total RNA including miRNA from biological samples and employs an extra column for the efficient removal of contaminating gDNA, thereby replacing the enzymantic DNase step.
Efficiently extract total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
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| Kit Specifications | |
| Maximum Binding Capacity Per Column | Up to 50 μg RNA |
| Maximum Loading Volume Per Spin Column | 650 μL |
| Size of RNA Purified | All sizes, including < 200 nt |
| Time to Complete 10 Purifications | 25 minutes |
| Maximum Amount of Starting Material | |
| Liver Tissue | 20 mg |
| Heart Tissue | 5 mg |
| Kidney Tissue | 20 mg |
| Brain Tissue | 25 mg |
| Lung Tissue | 20 mg |
| Spleen Tissue | 20 mg |
| Whole Blood | 100 µL |
| Hela Cells | 3 x 106 |
| CHO | 3 x 106 |
| Yeast | 1 x 108 cells |
| E.coli | 1 x 109 CFU/mL |
| Bacteria | 1 x 109 cells |
| Plasma/Serum | 200 µL |
| Fungi | 50 mg |
| Plant Tissue | 50 mg |
| Viruses | ≤100 µL of viral suspension |
| Average Yield | |
| HeLa Cells (1 x 106 cells)E. coli (1 x 109 cells)15 µg50 µg | |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 48300 (50 preps) | Cat. 48400 (100 preps) |
|---|---|---|
| Buffer RL | 40 mL | 2 x 40 mL |
| Wash Solution A | 38 mL | 2 x 38 mL |
| Elution Solution A | 6 mL | 2 x 6 mL |
| RNA Purification Columns | 50 | 100 |
| Genomic DNA Removal Columns | 50 | 100 |
| Collection Tubes | 100 | 200 |
| Elution Tubes (1.7 mL) | 50 | 100 |
| Product Insert | 1 | 1 |
