N-t-Boc-Aminooxy-PEG4-N-(PEG2-Propargyl) is a click chemistry crosslinker. The propargyl group is reactive with azide-containing compounds or biomolecules through copper catalyzed Click Chemistry to yield a stable triazole linkage. t-Boc-aminooxy can be deprotected under mild acidic conditions. The hydrophilic PEG linker improves solubility in aqueous media.
N-t-Boc-Aminooxy-PEG4-N-(PEG2-Propargyl) is a click chemistry crosslinker. The propargyl group is reactive with azide-containing compounds or biomolecules through copper catalyzed Click Chemistry to yield a stable triazole linkage. t-Boc-aminooxy can be deprotected under mild acidic conditions. The hydrophilic PEG linker improves solubility in aqueous media.
Cell Culture Plate 6 Wells
Cell Culture Plate provide excellent smoothness and uniformity based on accurate molding technology. By such property, clear view can be available when examine with a microscope.
Specifications: 6wells
12wells, 24wells., 48wells, 96wells
PRODUCT FEATURES
For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.
For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.
Cell Culture Plate provide excellent smoothness and uniformity based on accurate molding technology. By such property, clear view can be available when examine with a microscope.
Specifications: 6wells
12wells, 24wells., 48wells, 96wells
HiPure RNA Clean Up Kit provides a rapid and easy method for the purification and concentrate RNA from enzymatic reactions or for desalting the RNA samples. RNA purified using HiPure RNA Clean Up Kit is ready for all downstream applications such as RT-PCR, Northern blotting, mRNA purification, nuclease protection, and in vitro translation.
Specifications
| Features | Specifications |
| Main Functions | Purification and concentration of RNA（mRNA>200nt or miRNA）from transcription products, DNase cleavage products, labeled products, and crude RNA products |
| Applications | Sequencing, ligation, enzyme digestion, RT-PCR, labeling, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Crude RNA, enzymatic reaction solution |
| Sample amount | ≤100μl |
| Recovery | 90% |
| Elution volume | ≥15μl |
| Time per run | ≤15 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
The HiPure system uses a simple bind-wash-elute procedure. Binding buffer is added directly to the sample or other enzymatic reaction, and the mixture is applied to the column. Nucleic acids adsorb to the silica membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure RNA is eluted with a small volume of low-salt buffer provided or water, ready to use in all subsequent applications.
Advantages
Kit Contents
| Contents | R214402 | R214403 |
| Purification Times | 50 Preps | 250 Preps |
| Buffer GXP | 30 ml | 120 ml |
| Buffer RW2 | 20 ml | 2 x 50 ml |
| RNase-Free Water | 20 ml | 60 ml |
| HiPure RNA Mini Columns I | 50 | 250 |
| 2 ml Collection Tubes | 50 | 250 |
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
HiPure RNA Clean Up Kit provides a rapid and easy method for the purification and concentrate RNA from enzymatic reactions or for desalting the RNA samples. RNA purified using HiPure RNA Clean Up Kit is ready for all downstream applications such as RT-PCR, Northern blotting, mRNA purification, nuclease protection, and in vitro translation.
Biotin-PEG3-alkyne is a biotin PEG reagent with an alkyne group that enables click reaction with azido molecules in the presence of Cu catalyst. The hydrophilic PEG spacer arm imparts water solubility that is transferred to the biotinylated molecule.
Biotin-PEG3-alkyne is a biotin PEG reagent with an alkyne group that enables click reaction with azido molecules in the presence of Cu catalyst. The hydrophilic PEG spacer arm imparts water solubility that is transferred to the biotinylated molecule.
