N-(t-butyl ester-PEG2)-N-bis(PEG2-propargyl)

Overview

• Detection kits for the BKV/JCV
• Available in TaqMan format for analysis

The BK virus is a member of the polyomavirus family. It has been suggested that this virus may be transmitted through respiratory fluids or urine, since infected individuals periodically excrete virus in the urine. BK viral infections are typically asymptomatic in healthy individuals, however very mild symptoms may appear including mild respiratory infections and fever. Infections with BK virus in immunocompromised or immunosupressed patients are much more severe and may involve renal dysfunction. In fact, in kidney transplant patients the immunosupressive drugs required for the transplant may allow the virus to replicate within the graft, resulting in a disease called BK virus nephropathy (BKVN). The JC virus is a type of human polyomavirus and is very common in the general population, infecting 70 to 90% of humans. Most people acquire JCV in childhood or adolescence. Typically the infection is subclinical and no of consequence in individuals with healthy immune systems. The initial site of infection may be the tonsils or the gastrointestinal tract, and the virus then remains latent in the gastrointestinal tract. JCV can also infect the tubular epithelial cells in the kidneys, where it continues to reproduce, shedding virus particles in the urine. Also, JCV can cross the blood-brain barrier into the central nervous system. JCV is known to cause the usually fatal progressive multifocal leukoencephalopathy (PML) by destroying oligodendrocytes in the brain in immunodeficient or immunosuppressed individuals. The JC and BK viruses are very similar, with their genomes sharing 75% homology. It is however important to differentiate between the viruses due to the differences in pathology and especially the invariably fatal outcome of PML which is only caused by the JC virus.

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

Component	Cat. TM39350 (100 preps)	Cat. TM39310 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
BKV/JCV Primer & Probe Mix	280 μL	280 μL
BKV/JCV Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Overview

Peelable heat sealing film which is optically clear. Seal is suitable for qPCR and optical applications.

• Heat sealing offers a 100% effective method for plate sealing for a complete seal integrity, as well as being quick and cost effective
• Our ClearASeal Peel is an optically clear laminate film forming a peelable seal to polypropylene, polyethylene, polystyrene, polycarbonate and cyclic olefin copolymer (COC) plates
• The optical clarity of this seal enables its use for sealing plates required for imaging use, including fluorescent detection methods such as qPCR and colorimetric assays
• The ClearASeal Peel forms a complete seal to a plate enabling both low temperature uses, including low temperature storage, and high temperature uses, such as PCR (when used with a pressurized heated lid)
• This seal demonstrates moderate solvent resistance and can be utilized for short term compound storage at room temperature
• This seal is available as sheets, for use with manual and semi-automated sealers, such as our HeatASeal 500 Sealing Machine
• Also available in multiple roll formats compatible with specified automated heat sealers, such as our Wasp or Chameleon XT
• Our sheet seals are inter-leaved with paper sheets to help denote which side is the sealing side and aid removal of one sheet at a time from the pack

Peelable heat sealing film which is optically clear. Seal is suitable for qPCR and optical applications.

Propargyl-PEG13-bromide is a heterobifunctional reagent that can participate in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage. The bromide (Br) can be used in nucleophilic substitution reactions. The PEG units increase water-solubility of the molecule in in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG13-bromide is a heterobifunctional reagent that can participate in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage. The bromide (Br) can be used in nucleophilic substitution reactions. The PEG units increase water-solubility of the molecule in in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.