N-(t-butyl ester-PEG2)-N-bis(PEG2-propargyl)

Overview

• For use from preserved saliva collected with Norgen’s Saliva RNA Collection and Preservation Devices Dx (Cat. 53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes Dx (Cat. Dx69200)
• CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
• Fits into in vitro diagnostic workflows
• Isolate high-quality total RNA, including viral RNA, from fresh and preserved saliva and swab samples
• Fast and easy processing using rapid spin column format
• Isolate total RNA, from large rRNA down to microRNA (miRNA)
• No phenol or chloroform extractions
• Very sensitive & linear down to a few cells/viral copies without the need for carrier RNA
• Buffer chemistry inactivates viruses including SARS-CoV-2 – Explore the Application Note
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

These kits provides a rapid method for the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva collected on Norgen’s Saliva RNA Collection and Preservation Devices Dx (Cat. 53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes Dx (Cat. Dx69200). Purification is based on using Norgen’s proprietary resin separation matrix. RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The chemistry employed in the kit allows the purification of total RNA, including viral and bacterial RNA, irrespective of size or GC content. The purified RNA is ideal for in vitro diagnostic use for medical purposes.

Saliva/Swab RNA Purification Kit Dx (Spin Column)

This kit is optimized to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Norgen’s Saliva / Swab RNA Purification Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.

To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used.

Norgen’s Saliva/Swab RNA Purification Kit Dx is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques.

Norgen’s Saliva/Swab RNA Purification Kit Dx does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.

Saliva/Swab RNA Purification 96-Well Kit Dx (High Throughput)

This kit is optimized to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Norgen’s Saliva / Swab RNA Purification 96-Well Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings. To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used. Norgen’s Saliva / Swab RNA Purification 96-Well Kit Dx is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques. Norgen’s Saliva / Swab RNA Purification 96-Well Kit Dx does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.

• High throughput 96-well plate format for rapid purifications
• Very consistent well-to-well RNA isolation
• Bind & elute all RNA irrespective of size or GC content, without bias

Details

Supporting Data

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Kit Specifications
Sample Volume Range	250 µL
Size of RNA purified	All sizes, including small RNA (<200 nt)
Minimum Elution Volume	75 µL
Maximum Elution Volume	100 µL
Time to complete 96 purifications	30 minutes
Average Yield	≥ 1 µg **varies from sample to sample

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.  All solutions and plastics can be used until the expiration date specified on their labels. 

Component	Cat. Dx69100 (50 preps)	Cat. Dx69300 (192 preps)
Lysis Buffer A	30 mL	100 mL
Solution WN	18 mL	55 mL
Wash Solution A	18 mL	2 x 38 mL
Elution Solution A	6 mL	20 mL
Mini Spin Columns	50	–
96-Well Isolation Plate (Deep Well)	–	2
Collection Tubes	50	–
96-Well Collection Plate (Deep Well)	–	2
Elution Tubes (1.7 mL)	50	–
96-Well Elution Plate (Deep Well)	–	2
Adhesive Tape	–	4
Product Insert	1	1

Description 

1X Tris-Glycine-SDS Running Buffer Powder is used for electrophoresis on Q-PAGE™ TGN Precast Gel or Laemmli Tris-HCl gels in Tris-Glycine buffer system. It is convenient and universal for electrophoresis in Tris-Glycine buffer system.

Features

• Reliable: Rigorous quality control for reproducible separation of protein electrophoresis.

• Convenient: Premeasured pouches make 1 liter of 1X buffer solution; No pH adjustment is necessary. 

• Fast: Dissolving in minutes and then ready to use.

• Stable: Powder packaging suitable for long-term storage.

Contents 

0.025 M Tris, 0.192 M glycine, 0.10% SDS

Applications 

Running buffer for Laemmli Tris-HCl gel electrophoresis

Storage

Room temperature for 24 months 

1X Tris-Glycine-SDS Running Buffer Powder is used for electrophoresis on Q-PAGE™ TGN Precast Gel or Laemmli Tris-HCl gels in Tris-Glycine buffer system. It is convenient and universal for electrophoresis in Tris-Glycine buffer system.

endo-BCN-PEG2-alcohol is a click chemistry PEG linker. The BCN group is very reactive with azide-tagged molecules. The hydroxyl group enables further derivatization or replacement with other reactive functional groups. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

endo-BCN-PEG2-alcohol is a click chemistry PEG linker. The BCN group is very reactive with azide-tagged molecules. The hydroxyl group enables further derivatization or replacement with other reactive functional groups. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.