N-(t-butyl ester-PEG2)-N-bis(PEG2-propargyl) is a multi-functional PEG linker with two terminal propargyl groups and a t-butyl ester. The propargyl groups enables formation of triazole linkage with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry. The t-butyl protected carboxyl group can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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N-(t-butyl ester-PEG2)-N-bis(PEG2-propargyl) is a multi-functional PEG linker with two terminal propargyl groups and a t-butyl ester. The propargyl groups enables formation of triazole linkage with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry. The t-butyl protected carboxyl group can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
N-t-Boc-Aminooxy-PEG4-N-(PEG2-Propargyl)
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Product Info
N-t-Boc-Aminooxy-PEG4-N-(PEG2-Propargyl) is a click chemistry crosslinker. The propargyl group is reactive with azide-containing compounds or biomolecules through copper catalyzed Click Chemistry to yield a stable triazole linkage. t-Boc-aminooxy can be deprotected under mild acidic conditions. The hydrophilic PEG linker improves solubility in aqueous media.
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N-t-Boc-Aminooxy-PEG4-N-(PEG2-Propargyl) is a click chemistry crosslinker. The propargyl group is reactive with azide-containing compounds or biomolecules through copper catalyzed Click Chemistry to yield a stable triazole linkage. t-Boc-aminooxy can be deprotected under mild acidic conditions. The hydrophilic PEG linker improves solubility in aqueous media.
Lyophilized format designed for room temperature shipping
Available in TaqMan format for analysis
Norgen’s Clostridium difficile TaqMan PCR Lyophilized Kit is designed for the detection of Clostridium difficile specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
Norgen’s Clostridium difficile TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of Clostridium difficile specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
All kit components should be stored at -20°C upon arrival.
Once reconstituted, repeated thawing and freezing (>2 times) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Each kit is provided with 4 tubes of 2X PCR Master Mix and each tube is enough to run 25 reactions. It is not necessary to reconstitute all Master Mix tubes at once. The Master Mix tubes can be reconstituted as and when needed.
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
NGS DNA Fragmentation & Library Prep Kit (illumina and MGI Platforms)
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Product Info
The NGS DNA Fragmentation & Library Prep Kit (illumina and MGI Platforms) was developed for construction of high quality libraries for next generation sequencing. The kit uses intact genomic DNA (EDTA-free DNA or DNA resuspended in TE buffer) as input DNA without an additional DNA fragmentation step. Our technology provides a fast and simple workflow. DNA libraries can be generated around 2 hours with only 10 min hands-on time. Library multiplexing is possible.
NGS DNA Fragmentation & Library Prep Kit Workflow
The incorporation of DNA fragmentation in the kit makes it possible to directly use intact genomic DNA as input DNA without the need of mechanical DNA shearing or enzymatic DNA fragmentation. The NGS DNA Fragmentation & Library Prep Kit does not generate sequencing bias as compared to library using mechanical sheared DNA as input. Sequence coverage is also consistent between enzymatic shearing and mechanical shearing. The library size is inversely correlated with the incubation time of step 1 at 20°C.
Three index types are available for the kit of the illumina platform:
Non-index (Cat.# 30026): Libraries do not have index.
Index (Cat.# 30028): Each of the index primers contains a unique index sequence of 6 bases. Library multiplexing for 48 samples is possible. Index information can be downloaded here.
Unique dual index (Cat.# 30030): Library multiplexing for 96 samples is possible. With the unique features of our 4-Base Difference Index System, the index sequence is 8-base long and each index has 4 bases different from others. Our unique dual index primers effectively identify sequencing errors such as index hopping, mis-assignment of reads, and de-multiplexing errors etc. The unique dual index primers set consists of 96 pre-mixed unique pairs of index primers. Index information can be downloaded here.
Indexes are available for the MGI platform kits (Cat.# 34028).
Kit features:
1.5-hour protocol from intact genomic DNA to NGS library
Intact genomic DNA as input, DNA fragmentation is not needed.
Works with both EDTA-free DNA and DNA resuspended in TE buffer
Library conversion efficiency: 100 ng, 300 ng and 500 ng of intact genomic DNA were used as input.
The library size is inversely correlated with the incubation time of step 1 at 20°C.
NGS data comparison: enzymatic shearing versus mechanical shearing
Enzymatic shearing • DNA shearing and library prep: BioDynami NGS DNA Fragmentation & Library Prep Kit Mechanical shearing • DNA shearing: Covaris sonication • Library prep: BioDynami NGS DNA Library Prep Kit.
Document
The NGS DNA Fragmentation & Library Prep Kit (illumina and MGI Platforms) was developed for construction of high quality libraries for next generation sequencing. The kit uses intact genomic DNA (EDTA-free DNA or DNA resuspended in TE buffer) as input DNA without an additional DNA fragmentation step. Our technology provides a fast and simple workflow. DNA libraries can be generated around 2 hours with only 10 min hands-on time. Library multiplexing is possible.