Primer and probe mix (150 reactions) Reverse Transcription, target specific primers (RNA genome viruses only) Copy number standard curve (sufficient for multiple standard curves) Internal extraction control – Read through VIC channel* Endogenous control (150 tests) RNAse/DNAse free water *alternative fluorophores available on request
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Other Products
D3125 HiPure DNA Micro Kit
Product Info
Document
Product Info
Introduction
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from small volume of blood, tissue and dry blood spots.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 1-10μl blood, <10mg tissue, urine, blood stain, seminal stain
Applications
PCR, southern bolt and virus detection, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Animal tissues, blood stain, urine, seminal stain and various forensic samples
Sample amount
Blood:1-100μl, Tissue:<10mg
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column
Principle
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA).
Advantages
Fast – several samples can be extracted in 20 minutes (after digestion)
High purity – purified DNA can be directly used in various downstream applications
High recovery – DNA can be recovered at the level of PG
Good repeatability – silica technology can obtain ideal results every time
Kit Contents
Contents
D312502
D312503
Purification Times
50 Preps
250 Preps
Buffer ATL
15 ml
60 ml
Buffer AL
15 ml
60 ml
Buffer GW1*
22 ml
66 ml
Buffer GW2*
20 ml
2 x 50 ml
Carrier RNA
310 μg
2 x 310 µg
Proteinase K
24 mg
120 mg
Protease Dissolve Buffer
1.8 ml
10 ml
Buffer AE
15 ml
60 ml
HiPure DNA Mini Columns I
50
2 x 125
2 ml Collection Tubes
100
5 x 100
Storage and Stability
Carrier RNA and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.
Experiment Data
Document
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from small volume of blood, tissue and dry blood spots.
Not all cyanobacterial strains produce toxins. However, the toxin-producing strains cannot be distinguished from the nontoxin-producing strains by traditional light microscopy, commonly used to monitor water bodies. An alternative for the differentiation of potentially toxic strains from nontoxic strains is to use molecular methods to detect the presence of toxin biosynthetic genes. Such methods are already available and could be used for the detection and identification of potential microcystin and nodularin producers present in environmental samples (Attogene catalog number NA2024).
Screening for the toxin itself, can be very costly. In turn, real time PCR for the detection of the anaC gene in cyanobacterial strains and environmental samples can be a key indicator for the prescense of cyanobacteria capable of expressing the Anatoxin toxin. Attogen has thus, designed primer pairs and probes targeting a the conserved anaC gene region in order to enable the amplification and detection of several producer genera using real time PCR. Screening for the toxin genes can save significant costs and act as a triage for samples needing to be analyzed for the toxin itself.
Document
Real time qPCR kit For screening Anatoxin gene cluster Use in combination with Attogene Algae DNA isolation kit
Preservation and isolation of both cf-DNA and cf-RNA from a single tube
Fixative-free preservative, no cross-linking of DNA
Preserve cf-DNA/ct-DNA for 30 days at ambient temperature and for up to 8 days at 37°C
Preserve cf-RNA for 30 days at ambient temperature
Preserve Circulating Tumour Cells (CTCs) for 14 days at ambient temperature
No plasma volume loss after shipping/transportation
Prevent hemolysis allowing better separation of plasma
Prevent apoptosis of blood cells and fragmentation of genomic DNA
Produce high quality/quantity of plasma cf-DNA/ct-DNA/cf-RNA
Vacuumed to draw 8.7 mL of blood in 10 mL tubes
The procedure for plasma isolation using this kit is compliant with ISO 20186-3:2019
Norgen’s cf-DNA/cf-RNA Preservative Tubes are closed, evacuated plastic tubes for the collection and the preservation of cf-DNA, circulating tumor DNA, cf-RNA and circulating tumor cells in human whole blood samples during storage and shipping. Using these tubes in combination with Norgen’s Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen’s Plasma/Serum RNA Purification Kits enables the purification and concentration of an inhibitor-free cf-DNA/ct-DNA/cf-RNA in a very small elution volume. Plasma recovered from Norgen’s cf-DNA/cf-RNA Preservative Tubes is also compatible with any other cf-DNA and/or cf-RNA purification method. The purified cf-DNA, ct-DNA and cf-RNA are compatible with any downstream application assays, including PCR, qPCR, rt-qPCR, methylation-sensitive PCR, Southern Blot analysis, gene expression analysis, microarrays and NGS.
This product is for research use only and not for use in diagnostic procedures.
Performance
Norgen’s cf-DNA/cf-RNA Preservative Tubes abridge the collection/processing of whole blood for the subsequent purification of cf-DNA and/or cf-RNA. The cf-DNA/cf-RNA Preservative Tubes preserve and inhibit the programmed cell-death (apoptosis) of the blood, hence preventing the release of intracellular DNA/RNA into plasma. Whole blood samples can be shipped and stored at room temperature for the subsequent processing of plasma. The cf/ct DNA and cf-RNA levels are stable for up to 30 days at room temperature. The cf-DNA is also stable for up to 8 days at 37°C. When blood collected on Norgen’s cf-DNA/cf-RNA tubes is processed for plasma recovery no buffy coat will be generated and the Circulating tumor cells (CTCs) will be located at the bottom of the tube with the blood sediment. CTCs are stable for 14 days at room temperature where it can be extracted from the blood sediment or from whole preserved blood for cell sorting or for cf/DNA or cf-RNA purification.
Plasma samples produced from the whole blood sample collected into Norgen’s cf-DNA/cf-RNA Preservative Tubes and purified using Norgen’s Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen’s Plasma/Serum RNA Purification Kits yield concentrated, high-quality and inhibitor-free cf-DNA/ct-DNA or cf-RNA for any downstream application.
Workflow
NOTE: This product is for research use only. For the CE marked version, see Cat. Dx63950
Up to 30 days at room temperature (15-25°C) Up to 8 days at 37°C
Length of cf-RNA Preservation
Up to 30 days at room temperature (15-25°C)
Length of CTCs Preservation
Up to 14 days at room temperature (15-25°C)
Storage Conditions and Product Stability
When stored at 15-30°C, unfilled Norgen cf-DNA/cf-RNA Preservative Tubes are stable through the expiration date. See tube label for expiry date.
Short-term storage/shipping at -20°C to 37°C for up to 10 days is acceptable for unfilled Norgen cf-DNA/cf-RNA Preservative Tubes.
Do not freeze unfilled Norgen cf-DNA/cf-RNA Preservative Tubes for long term storage over 10 days. Freezing unfilled tubes may lead to loss of vacuum and precipitation may occur.
Cell-free DNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes is stable for up to 30 days when stored at room temperature (15-25°C)
Cell-free DNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes is stable for up to 8 days when stored at 37ºC.
Cell-free RNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes is stable for up to 30 days when stored at room temperature (15-25°C)
Circulating tumor cells (CTCs) in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes are stable for up to 14 days at room temperature (15-25ºC).
