Neisseria gonorrhoeae

Ideal magnet for all of your low volume needs. From Low elution 96-well PCR applications to 384-well, the X396 has  you covered. No need to purchase two separate plates anymore. 96-well volumes as low as 5 µL are achieved from 96-well, PCR plates on the inside of the magnet, while the outside of each magnet will pull beads to well sides in 384-well plates.

ANSI/ SBS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot on bottom. ANSI/SBS footprint on top to accept all common microplates.

Integrated Cushion base for maximum recovery. Helps aid in set-up, robot positioning inconsistencies, and labware consumable inconsistencies

Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads

SKU #

A040523

Features

• ANSI/ SBS footprint
• Compatible with 384-well and 96-well plates
• Solid Aluminum alloy design
• Precision manufactured for more consistent results
• Fast magnetic bead separations
• Made in USA 
• For Research use only

Compatibility

• 384-well pcr plates
• Some 384-well deep well plates
• Full-, Semi-, and un-Skirted 96-well PCR Plates
• Any magnetic beads
• Any common liquid handlers i.e. Tecan®, Opentrons®, Hamilton®, Agilent®, Beckman®, etc.

Volumes

384-Well PCR

Maximum – 39 µL 

Minimum – 5 µL 

__________

96-Well PCR

Maximum – 60 µL (0.2 mL for some beads)

Minimum – 4 µL

Ideal magnet for all of your low volume needs. From Low elution 96-well PCR applications to 384-well, the X396 has you covered. No need to purchase two separate plates anymore. 96-well volumes as low as 5 µL are achieved from 96-well, PCR plates on the inside of the magnet, while the outside of each magnet will pull beads to well sides in 384-well plates.

Overview

• Columns bind proteins of interest while endotoxins flow through
• Reduce endotoxin levels to less than 0.01 EU/µg of protein
• Proteins are desalted
• Greater than 95% protein recovery
• Concentrate protein samples and remove detergents at the same time
• Effectively remove a wide range of detergents including SDS, Triton® X-100, CHAPS, NP-40, and Tween 20
• Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Kits are designed for the rapid removal of endotoxins from previously purified proteins or peptides, with protein recoveries of > 95% being achieved. Endotoxins, also known as lipopolysaccharides, are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins liberated by Gram-negative bacteria are frequent contaminations of protein solutions derived from bioprocesses. Due to the high toxicity of endotoxins in vivo and in vitro, their removal from protein preparations is often necessary prior to the use of the protein in downstream applications. These kits efficiently reduce endotoxin levels to ≤ 0.01 EU/mg of protein, using spin column chromatography based on Norgen’s proprietary resin as the separation matrix. These kits are highly efficient in removing many different salts commonly used in the laboratory including, but not limited to, MgCl₂, NaCl, KCl, CaCl₂, LiCl and CsCl. The purified protein samples can be used in a number of downstream applications including sequencing, cloning, and in vitro and in vivo introduction into cells and organisms for various purposes. The simultaneous removal of salts while concentrating a dilute protein solution makes the kit a convenient method for preparing proteins before running many downstream applications such as SDS-PAGE, isoelectric focusing, X-ray crystallography, NMR spectroscopy, mass spectroscopy and other applications.

Mini Kit

The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit is designed for the rapid removal of endotoxins from up to 200 μg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.

Maxi Kit

The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit is designed for the rapid removal of endotoxins from up to 4 mg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.

Details

Supporting Data

Figure 1 / 15

Previous

Next

Click for expanded view

Kit Specifications
Maximum Protein Input	4 mg
Minimum Protein Input	0.25 mg
Maximum Column Volume Input	18 mL
Molecular Weight of Recovered Proteins	No Molecular Weight cut-off
Final Endotoxin Levels	≤ 0.01 EU/μg protein
Protein Recovery	90-95%
% Detergent Removal	90-95%
Detergents that can be Removed	Including Triton® X-100, CHAPS, NP-40 and Tween 20
Elution Volume	2-4 mL
Time to Complete 10 Purifications	30-40 minutes

Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 22800 (25 preps)	Cat. 22200 (4 preps)
Binding Buffer J	8 mL	8 mL
Binding Buffer N	4 mL	20 mL
Wash Solution M	50 mL	130 mL
Wash Solution CIP	20 mL	60 mL
Wash Solution N	30 mL	130 mL
Wash Solution NIP	20 mL	60 mL
Elution Buffer G	6 mL	20 mL
Endotoxin Removal Solution	1.5 mL	1.5 mL
Protein Neutralizer EF	2 mL	2 mL
Maxi Spin Columns (assembled with Collection Tubes)	–	4
Mini Spin Columns	25	–
Collection Tubes	25	–
Maxi Spin Columns (assembled with Collection Tubes)	4
Elution Tubes (1.7 mL)	25	–
Elution Tubes (50 mL)	4	–
Product Insert	1	1

Introduction

Usages:
For determination of total bacterial count .

Principle:
Tryptone provide carbon and nitrogen; yeast extract powder provides B vitamins; glucose to provide energy, agar is the solidifying agent.
Formulation(per liter):
Tryptone 5.0g
Yeast extract powder 2.5g
Glucose 1.0g
Agar 15.0g
Final pH 7.0 ± 0.2

How to use:
1.Suspend 23.5g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 autoclave for 15min.
2.Diluted and treated samples.

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

500g