NEST 1.5 mL Microcentrifuge Tube (500 count)

NH-bis(PEG4-Propargyl) is a bifunctional PEG compound containing two terminal alkynes that are joined together at a secondary amine. Terminal alkynes are reactive towards azides in copper (I) click chemistry to form stable triazoles with the target compound. The secondary amine joining the two arms can be used as a nucleophile such as in alkylation via reductive amination or in forming amides with carboxylic acids or activated NHS esters. The use of a central amine also allows for hydrogen bonding, further increasing this compound’s water solubility.

NH-bis(PEG4-Propargyl) is a bifunctional PEG compound containing two terminal alkynes that are joined together at a secondary amine. Terminal alkynes are reactive towards azides in copper (I) click chemistry to form stable triazoles with the target compound. The secondary amine joining the two arms can be used as a nucleophile such as in alkylation via reductive amination or in forming amides with carboxylic acids or activated NHS esters. The use of a central amine also allows for hydrogen bonding, further increasing this compound’s water solubility.

Overview

• Detection kits for Vibrio cholerae
• Available in TaqMan format for analysis
• CE-IVD marked version available for in vitro diagnostic use

Vibrio cholerae is a comma-shaped, gram-negative bacterium. It is the cause of cholera in humans, which affects the upper small intestine. Transmission of the disease is mainly through contaminated food or water. Human subjects affected by cholera exhibit severe watery diarrhea and vomiting, caused by the cholera toxin produced by the bacterium. Many cases of cholera are life-threatening, as diarrhea and associated vomiting can lead to rapid dehydration and electrolyte loss. Even with the extensive research on its epidemiology, cholera still affects over 5 million people per year worldwide.

Vibrio cholerae TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Vibrio cholerae TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM38550 (100 preps)	Cat. TM38510 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
V. cholerae Primer & Probe Mix	280 μL	280 μL
V. cholerae Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Description

Attogene’s Montmorillonite Bentonite Clay binds toxins with high affinity and high capacity.  Montmorillonite has been shown to be the active ingredient in bentonites.  The proposed mechanism of action of Attogene’s Montmorillonite Bentonite Clay is through the adsorption of toxins (mainly onto the interlayer spaces of montmorillonite).  Attogene’s Montmorillonite Bentonite Clay is a common anti-caking agent in animal feeds to adsorb aflatoxins and diminish their bioavailability. Its composite for application in drug system can be formed through the utilization of anionic, cationic, and nonionic surfactants.

Bentonite is a promising rock of clay which is found in nature. It is a major source of montmorillonite in nature. It is a rock produced of highly colloidal and plastic clays mainly comprised of montmorillonite. In addition to montmorillonite, bentonite may compose of some amount of crystalline quarz, cristobalite, and feldspar. Montmorillonite nanoclay is used as a drug carrier system and as an additive. Its composite for application in drug system can be formed through the utilization of anionic, cationic, and nonionic surfactants. These are used to improve basal spacing resulting in organo clay to be utilised in drug loading and drug release.

Promotes flocculation of cyanobacteria cell debris, binds residual phosphate compounds and promotes gradual settling of the cyanobacterial biomass on the sediment.

Sample Package Size: 500g