NEST 50 mL Centrifuge Tube, clear, polypropylene tube, high-density polyethylene cap, DNase, RNase, pyrogen and endotoxin free. Sterile. Printed graduations in marking area. 500 count.
Labware definition is available for immediate use in Opentron’s Labware Library.
NEST 50 mL Centrifuge Tube, clear, polypropylene tube, high-density polyethylene cap, DNase, RNase, pyrogen and endotoxin free. Sterile. Printed graduations in marking area. 500 count.
Labware definition is available for immediate use in Opentron’s Labware Library.
Norgen’s Exosome Purification and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the subsequent isolation of exosomal RNA from different urine sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is free from any protein-bound circulating RNA and of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Urine Exosome Purification and RNA Isolation Mini Kit
For sample volumes ranging from 250 µL to 1 mL.
Urine Exosome Purification and RNA Isolation Midi Kit
For sample volumes ranging from 2 mL to 10 mL.
Urine Exosome Purification and RNA Isolation Maxi Kit
For sample volumes ranging from 11 mL to 30 mL.
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| Kit Specifications | |
| Minimum Urine Input | 250 μL |
| Maximum Urine Input | 1 mL |
| Size of Exosomes Purified | 40 nm – 150 nm |
| Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
| Elution Volume | 50-100 μL |
| Time to Complete 10 Purifications | 35 – 40 minutes |
| Average Yields* | Variable depending on specimen |
*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.
| Component | Cat. 58400 (50 preps) | Cat. 58700 (25 preps) | Cat. 58800 (15 preps) |
|---|---|---|---|
| Slurry E | 12.5 mL | 12.5 mL | 12.5 mL |
| ExoC Buffer | 8 mL | 30 mL | 50 mL |
| ExoR Buffer | 12 mL | 12 mL | 12 mL |
| Lysis Buffer A | 20 mL | 20 mL | 20 mL |
| Lysis Additive B | 2 mL | 2 mL | 2 mL |
| Wash Solution A | 18 mL | 18 mL | 18 mL |
| Elution Solution A | 6 mL | 6 mL | 6 mL |
| Mini Filter Spin Columns | 50 | 25 | 15 |
| Mini Spin Columns | 50 | 25 | 15 |
| Collection Tubes | 50 | 25 | 15 |
| Elution Tubes (1.7 mL) | 100 | 50 | 30 |
| Product Insert | 1 | 1 | 1 |
This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Specifications
| Features | Specifications |
| Main Functions | IVD5412 precast kit for kingfisher Flex |
| Applications | RT-PCR，PCR，NGS |
| Products | Viral DNA / RNA, body cell DNA / RNA |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technolog |
| Process method | Manual or automatic |
| Sample type | |
| Sample amount | 200μl |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Nuclease Free Water.
Advantages
Kit Contents
| Cat.No | Reagent | IVD5412-F-96 |
| PK/Carrier RNA | 1x 24 mg/Bottle | |
| Protease Dissolve Buffer Blue | 1.8 ml/Bottle | |
| Tip | 1 | |
| Sample Plate (DW Plate) | 500µl Buffer MLB/Well | 1 |
| Wash 1 Plate (DW Plate) | 500µl Buffer MW1/Well | 1 |
| Wash 2 Plate (DW Plate) | 500µl Buffer CW/Well | 1 |
| Beads Plate (DW Plate) | 500µl CW & 20µl MPN/Well | 1 |
| Elute Plate (KF Plate) | 90µl NFW/Well | 1 |
Storage and Stability
This kit is shipped and stored at room temperature and is valid for 12 months.
This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
A rapid, high performance dye-terminator removal process based on the paramagnetic bead technology. The paramagnetic bead format requires no centrifugation or filtration and is easily performedmanually or fully automated for high throughput dye-terminator removal. Compared to similar systems, this product produces sequences with longer Phred 20 read lengths and higher signal intensities than any other purification technology.
Specifications
| Features | Specifications |
| Main Functions | Removal of free fluorescent dye from sequencingsolution (Replace Beckmen or agencourt CleanSeq) |
| Applications | Automated sequences |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | DNA doped with fluorescentdye |
| Sample amount | 5μl |
| Recovery | 90% |
| Elution volume | ≥25μl |
| Operation time | ≤50 minutes |
The CleanSeq method contains magnetic particles in an optimized binding buffer to selectively capture sequencing extension products. The protocol can be performed directly in the thermal cycling plate. Unincorporated dyes, nucleotides, salts and contaminants are removed using a simple washing procedure. The purification procedure is amenable to a variety of automation platforms since it requires no centrifugation or vacuum filtration.
Advantages
Kit Contents
| Contents | BCS-5 | BCS-50 | BCS-500 |
| CleanSeq Beads | 5 ml | 50 ml | 500 ml |
Storage and Stability
CleanSeq Beads should be stored at 2-8°C upon arrival and is stable up to 6 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. Mix CleanSeq Beads well before using. The reagent should appear homogenous and consistent in color.
DO NOT FREEZE.
A rapid, high performance dye-terminator removal process based on the paramagnetic bead technology. The paramagnetic bead format requires no centrifugation or filtration and is easily performedmanually or fully automated for high throughput dye-terminator removal. Compared to similar systems, this product produces sequences with longer Phred 20 read lengths and higher signal intensities than any other purification technology.
