Content:	100 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Histamine
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	492
Signal Response:	Increase
Linear Range:	0.5 to 12 μg of histamine per assay
Limit of Detection:	~ 0.09 μg of histamine per test or ~ 0.75 mg/Kg (ppm) of histamine in a fish sample
Limit of Quantification:	~ 0.5 mg of histamine per test or  ~ 4.2 mg/Kg (ppm) of histamine in a fish sample
Reproducibility (%):	~ 3%
Reaction Time (min):	~ 20 min
Application examples:	Fish samples in various forms (e.g. fresh, canned, frozen, etc).

This product has been discontinued.

The Histamine Assay Kit for the detection of histamine in food including fish samples in various forms.

High levels of histamine may develop in a variety of foods as they decompose, this is an issue that particulary affects fish.

Megazyme’s Histamine Assay Kit provides a simple robust method with a fast reaction time of ~ 20 mins.

View all of our assay kits.

Advantages

• Very competitive price (cost per test)
• All reagents stable for > 2 years
• Specific for histamine
• Suitable for use with raw fish samples
• Simple format. Well suited to automation
• Standard included
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing

The Histamine Assay Kit for the detection of histamine in food including fish samples in various forms.

Introduction

This kit provides a simple and fast solution for the extraction of circulating nuclear acid from serum, plasma, and other cell-free liquid samples. Circulating nucleic acid refers to the free extracellular nucleic acid produced by cell apoptosis, of which fragments are generally below 1KB. The kit is based on silica gel column purification technology, which is no need for toxic phenol chloroform extraction and time-consuming alcohol precipitation during the extraction. The obtained Circulating Nucleic Acid can be directly used for quantitative PCR, liquid or solid phase chip analysis, hybridization, and SNP detection.
HiPure Circulating DNA/RNA Kit adopts a unique solution system and multiple layers of filter membranes with different pore sizes, which can efficiently process large volumes of serum and plasma samples and capture extremely small amounts of free nucleic acids.

Details

Specifications

Features	Specifications
Main Functions	Isolation both Circulating DNA/RNA (include miRNA) from 1-5 ml serum and plasma
Applications	qPCR / RT-PCR, liquid or solid-phasechip analysis, hybridization and SNP detection
Purification method	Midi spin column
Purification technology	Silica technology, DNA filtration technology
Process method	Manual (centrifugation or vacuum)
Sample type	serum, plasma, and other cell-free liquid samples
Sample amount	1-5 ml
Elution volume	≥20μl
Time per run	≤100 minutes
Liquid carrying volume per column	4 ml
Binding yield of column	1 mg

Principle

This kit is based on silica gel column technology. Serum or other liquid samples are lysed and digested in buffer CFL. After adding buffer CFP, the protein is removed by centrifugation to obtain the supernatant. Isopropanol is added to precipitate the total nucleic acid and transferred to the column for filtration. DNA / RNA is adsorbed on the membrane of the column, while the protein is not adsorbed and removed with the filtrate.The column is washed with buffer MGW1 to remove protein and other impurities, and then washed with buffer RW2 to remove salt. Finally, DNA / RNA is eluted by low salt buffer. The eluted DNA / RNA can be directly used for quantitative PCR/ RT-PCR, liquid or solid-phase chip analysis, hybridization and SNP detection.

Advantages

• High yield – most optimized process to obtain maximum free RNA and small RNA
• High concentration – low elution volume (>20μl) to ensure nucleic acid concentration
• High purity – low alcohol combination, completely remove inhibitor and protein pollution
• High recovery – silica gel column technology can recover nucleic acid molecules at the level of PG
• Large volume – 1-2ml serum and plasma samples can be processed at one time

Kit Contents

Contents	R431602	D431603
Purification Times	50 Preps	250 Preps
HiPure RNA Micro Columns	50	5 x 50
HiPure Viral Midi Columns	50	5 x 50
15 ml Collection Tubes	50	5 x 50
2ml Collection Tubes	50	5 x 50
Buffer CFL	150 ml	2 x 375 ml
Buffer CFP	30 ml	150 ml
Buffer MGW1*	100 ml	2 x 250 ml
Buffer RW2*	2 x 50 ml	5 x 100 ml
RNase Free Water	10 ml	50 ml

Storage and Stability

The kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

This kit provides a simple and fast solution for the extraction of circulating nuclear acid from serum, plasma, and other cell-free liquid samples. Circulating nucleic acid refers to the free extracellular nucleic acid produced by cell apoptosis, of which fragments are generally below 1KB. The kit is based on silica gel column purification technology, which is no need for toxic phenol chloroform extraction and time-consuming alcohol precipitation during the extraction. The obtained Circulating Nucleic Acid can be directly used for quantitative PCR, liquid or solid phase chip analysis, hybridization, and SNP detection.
HiPure Circulating DNA/RNA Kit adopts a unique solution system and multiple layers of filter membranes with different pore sizes, which can efficiently process large volumes of serum and plasma samples and capture extremely small amounts of free nucleic acids.

Our 384-well PCR plates have a rigid fully-skirted frame which remains stable during thermal cycling.

About

Our 384-well plate has a rigid, fully-skirted frame which remains stable during thermal cycling.
The rigidity of the plate lends itself to automated and high-throughput PCR environments where robotic handling maybe in use.

The 2-component design reduces the risk of evaporation due to a poor sealing, commonly seen with 1-component PCR plates. 100% virgin medical grade polypropylene is used for the 384 wells, with a maximum capacity of 45 µL Certified free from DNase, RNase, nucleases and human gDNA.

Compatible with a wide range of PCR and qPCR applications, for a full list see ‘Plate Instrument Compatibility List’.

Available in clear or white well format.

Our 384-well PCR plates have a rigid fully-skirted frame which remains stable during thermal cycling.