NEST 50 mL Centrifuge Tube, clear, polypropylene tube, high-density polyethylene cap, DNase, RNase, pyrogen and endotoxin free. Sterile. Printed graduations in marking area. 500 count.
Labware definition is available for immediate use in Opentron’s Labware Library.
NEST 50 mL Centrifuge Tube, clear, polypropylene tube, high-density polyethylene cap, DNase, RNase, pyrogen and endotoxin free. Sterile. Printed graduations in marking area. 500 count.
Labware definition is available for immediate use in Opentron’s Labware Library.
Norgen’s Total RNA Purification Maxi Kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants, and viruses. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Norgen’s Purification Technology
Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The process involves first lysing the cells or tissue of interest with the provided Buffer RL (please see the flow chart on page 4). Ethanol is then added to the lysate, and the solution is loaded onto a maxi spin column. Norgen’s resin binds RNA in a manner that depends on ionic concentrations. Thus only the RNA will bind to the column, while the contaminating proteins will be removed in the flowthrough or retained on the top of the resin. The bound RNA is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified total RNA is eluted with the Elution Solution A. The purified RNA is of the highest integrity, and can be used in a number of downstream applications.
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| Kit Specifications | |
| Maximum Column Binding Capacity | 1.5 mg |
| Maximum Column Loading Volume | 20 mL |
| Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
| Maximum Amount of Starting Material | |
| Liver Tissue | 100 mg |
| Heart Tissue | 50 mg |
| Kidney Tissue | 100 mg |
| Brain Tissue | 250 mg |
| Lung Tissue | 100 mg |
| Whole Blood | 2 – 10 mL* |
| Bacteria | 2.5 x 10 10 cells |
| Yeast | 2 x 108 cells |
| Fungi | 1 g |
| Plant Tissues | 1 g |
| Time to Complete 4 Purifications | 40 minutes |
| Average Yield: HeLa Cells (5 x 107 cells) E. coli (2.5 x 1010 cells) | ~750 μg ~1.5 mg |
*For isolating total RNA from purified leukocytes
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 26800 (8 preps) |
|---|---|
| Buffer RL | 2 x 40 mL |
| Wash Solution A | 4 x 38 mL |
| Elution Solution A | 3 x 20 mL |
| RNA Maxi Spin Columns (with collection tubes) | 8 |
| Elution Tubes (50 mL) | 8 |
| Product Insert | 1 |
Not all cyanobacterial strains produce toxins. However, the toxin-producing strains cannot be distinguished from the nontoxin-producing strains by traditional light microscopy, commonly
used to monitor water bodies. An alternative for the differentiation of potentially toxic strains from nontoxic strains is to use molecular methods to detect the presence of toxin biosynthetic genes. Such methods are already available and could be used for the detection and identification of potential microcystin and nodularin producers present in environmental samples (Attogene catalog number NA2024).
Screening for the toxin itself, can be very costly. In turn, real time PCR for the detection of the anaC gene in cyanobacterial strains and environmental samples can be a key indicator for the prescense of cyanobacteria capable of expressing the Anatoxin toxin. Attogen has thus, designed primer pairs and probes targeting a the conserved anaC gene region in order to enable the amplification and detection of several producer genera using real time PCR. Screening for the toxin genes can save significant costs and act as a triage for samples needing to be analyzed for the toxin itself.
Real time qPCR kit
For screening Anatoxin gene cluster
Use in combination with Attogene Algae DNA isolation kit
Product Description
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).
Technical Parameters:
| Parameters | Details |
|---|---|
| Product Name | DNA Isothermal Amplification Kit NFO |
| Manufacturer | Amp-future |
| Storage Temperature | -20°C |
| Kit Components | Enzymes, Buffers ,Reagents |
| Packaging | 48 Tests/box |
| Detection Limit | 500-1000copies/µL |
| Shipping | ICE |
| Test Time | 5-20mins |
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Isothermal nucleic acid Applications
Suitable for DNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
