NEST 50 mL Centrifuge Tube, clear, polypropylene tube, high-density polyethylene cap, DNase, RNase, pyrogen and endotoxin free. Sterile. Printed graduations in marking area. 500 count.
Labware definition is available for immediate use in Opentron’s Labware Library.
Detail
NEST 50 mL Centrifuge Tube, clear, polypropylene tube, high-density polyethylene cap, DNase, RNase, pyrogen and endotoxin free. Sterile. Printed graduations in marking area. 500 count.
Labware definition is available for immediate use in Opentron’s Labware Library.
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Giardia intestinalis TaqMan RT-PCR Detection Kits
Product Info
Document
Product Info
Overview
Detection kits for Giardia intestinalis
CE-IVD marked version available for in vitro diagnostic use
Available in TaqMan format for analysis.
Giardiasis is a disease of the small bowel caused by the protozoan parasite Giardia intestinalis (syn.duodenalis or lamblia). Giardia is one of the most common intestinal parasites in the world, and occurs at very high prevalence rates in places with poor water sanitation. Individuals become infected through ingesting or coming into contact with contaminated water, food or soil. It can also be spread through the faecal-oral route due to poor hygiene practices, which makes it common in day-care centers. G. intestinalis lives inside the intestines of infected humans or other animals including cats, dogs, birds, cows, beaver and deer. Symptoms of infection include diarrhea, malaise, excessive gas, bloating, nausea, diminished interest in food, possible vomiting and weight loss.
There are 2 kits available for the detection of Giardia intestinalis:
Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Usages: For isolating lactose-fermenting Gram-negative enteric bacilli.
Principle: Peptones are sources of nitrogen and other nutrients. Lactose is a fermentable carbohydrate. When lactose is fermented, alocal pH drop around the colony causes a color change in the pH indicator (neutral red) and bile precipitation. Bile salts,bile salts no. 3, oxgall and crystal violet are selective agents that inhibit growth of gram-positive organisms. Agar is the solidifying agent.
Formulation(per liter):
Pancreatic Digest of Gelatin
17.0 g
Peptones (meat and casein)
3.0 g
Lactose Monohydrate
10.0 g
Sodium Chloride
5.0 g
Bile Salts
1.5 g
Agar
13.5 g
Neutral Red
30.0 mg
Crystal Violet
1 mg
Final pH
7.1±0.2
How to use: 1.Suspend 50 g in 1 L of distilled or deionized water. Heat to boiling to dissolve completely. Autoclave at 121°C for 15 minutes.
2.Transfer 1 mL of Soybean–Casein Digest Broth to 100 mL of MacConkey Broth, and incubate at 42 to 44 for 24 to 48 hours. Subculture on a plate of MacConkey Agar at 30 to 35 deg.C for 18 to 72 hours.
Quality control:
Item
The name and number of strain
PR/G
Reaction
Growth rate
E.Coli ATCC8739
PR≥0.7
Rose-red
Characteristic difference
Proteus mirabilis CMCC(b)49005
PR≥0.7
Colorless, no swarming
Selective
Staphylococcus aureus ATCC6538
G≤1
-
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of three years.
Propargyl-PEG17-methane has an alkyne group which can participate in Click Chemistry reactions with azide compounds to yield a stable triazole linkage; copper is required as a catalyst. The hydrophilic PEG units increases solubility of the molecules in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG17-methane has an alkyne group which can participate in Click Chemistry reactions with azide compounds to yield a stable triazole linkage; copper is required as a catalyst. The hydrophilic PEG units increases solubility of the molecules in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.