NEST 96 Well Plate Flat, polystyrene, sterile, DNase, RNase, pyrogen, and endotoxin free. Individually wrapped.
100 count, 200 uL max volume per well
Labware definition is available for immediate use in Opentron’s Labware Library
NEST 96 Well Plate Flat, polystyrene, sterile, DNase, RNase, pyrogen, and endotoxin free. Individually wrapped.
100 count, 200 uL max volume per well
Labware definition is available for immediate use in Opentron’s Labware Library
NEST 96 Well Plate Flat, polystyrene, sterile, DNase, RNase, pyrogen, and endotoxin free. Individually wrapped.
100 count, 200 uL max volume per well
Labware definition is available for immediate use in Opentron’s Labware Library
The NGS Library Quantification Standards with PCR Primers (for Ion Torrent platform) were developed for quantifying the library concentration for ion torrent sequencing platform. Quantification of the library of the fully ligated libraries is important for the quality of the sequencing outcome. Optimal library concentration can increase sequencing yield. Poor library concentration results in bad emPCR, which can lead to low sequencing capacity.
QPCR is the best method for library quantification. Our reagent only amplifies library molecules that will be used for subsequent emPCR, and is optimized for amplification of various samples. Our reagent is compatible with commercial SYBR Green based QPCR reagents. Quantification of library concentration is achieved by comparison with a standard curve generated from DNA Standards.
The kit comprises DNA Standards (six 10-fold dilutions) and a primer mix.
NGS Library Quantification Standards with PCR Primers (Ion Torrent platform): real time quantitative PCR curve of the standards.
The NGS Library Quantification Standards with PCR Primers (for Ion Torrent platform) were developed for quantifying the library concentration for ion torrent sequencing platform. Quantification of the library of the fully ligated libraries is important for the quality of the sequencing outcome. Optimal library concentration can increase sequencing yield. Poor library concentration results in bad emPCR, which can lead to low sequencing capacity.
Usages:
For differentiating enteric bacteria based on urease activity by adding 40% sterile urea solution.
Principle:
Peptone provides the carbon and nitrogen; maintain a balanced osmotic sodium chloride; potassium dihydrogen phosphate is buffers; decomposing bacteria urease urea medium, produce large amounts of ammonia, agar as medium coagulant.
Formulation (per liter):
Peptone 1g
Sodium chloride 5g
Glucose 1g
Ppotassium dihydrogen phosphate 2g
Phenol red 0.012g
Agar 12g
Final pH7.2 ± 0.2
How to use:
1.Suspend 21g in 1L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121 for 15 minutes. cooling to 50-55 and adding 40% urea solution.
2.Diluted and treated samples.
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
500g
