Product Description

Comprehensive Monitoring & Early Detection Nucleic Acid Detection Bovine Viral Diarrhea Virus

Product Description:

The Livestock Disease Kit is a reliable and easy-to-use test kit designed to detect the presence of diseases in livestock, such as cattle, sheep, goats, pigs and horses. The kit has been designed to detect diseases with high accuracy and sensitivity. Also it requires only a small sample volume of 50 μl, making it highly efficient and cost-effective. It is also suitable for a range of canine dog test kit applications, providing fast and accurate results.The reliable detection results make it a reliable and cost-effective solution for farmers and veterinarians looking to diagnose livestock diseases quickly and accurately.

Features:

• Product Name: Bovine viral diarrhea virus isothermal detetction fluorescence kit
• Target Species:  Bovine
• Type: Fluorescence Kit
• Storage Temperature: -20°C
• Sample Type: Serum, Plasma, Whole Blood

Technical Parameters:

Product Name	Bovine viral diarrhea virus​ isothermal isothermal detetction fluorescence kit
Type	Fluorescence Kit
Sample Volume	50 μl
Sample Type	Serum, Plasma, Whole Blood
Storage Temperature	-20°C
Package Size	48 Tests/Kit
Target Disease	Livestock Disease
Target Species	Bovine

Applications:

Amp-future bio Livestock Disease Kit is an innovative animal health care product designed to detect and diagnose livestock diseases. Developed by a Chinese research team, this kit is a simple and reliable solution to test and treat livestock diseases. With its detection limit of 0.1 ng/ml, users can obtain accurate results quickly and accurately. The test time takes from 5-20 minutes, and the kit typically contains 48 tests/kit. Furthermore, it is easy to store with its cold storage temperature of -20°C.

The Amp-future bio Livestock Disease Kit provides an innovative solution for animal health care. It is an essential product for veterinary professionals, pet owners, and animal health researchers to detect and diagnose livestock diseases. With its simple and reliable detection and diagnosis, this kit is a must-have for anyone who wants to ensure the health of their animals.

Support and Services:

Livestock Disease Kit Technical Support and Services

We provide technical support and services for our Livestock Disease Kit. We are committed to helping you get the most out of your product and ensuring your satisfaction with our products and services.

• Our technical support staff is available to answer any questions you may have about the product.
• We offer online tutorials and user guides to help you understand the product better.
• We provide technical assistance to help troubleshoot any issues you may be having with the product.
• We offer a warranty on our products.

If you have any questions or need technical support, please do not hesitate to contact us. We are here to help you make the most of your Livestock Disease Kit.

FAQ:

• Q: What diseases can Livestock Disease Kit detect?
  A: Livestock Disease Kit can detect different infectious diseases, such as Foot and Mouth Disease, Bluetongue Disease, Bovine Viral Diarrhea.
• Q: How does Livestock Disease Kit work?
  A: Livestock Disease Kit uses advanced MIRA technology to amplify the target DNA/RNA targent sequences , and detect the disease by analyzing the amplified sequences.
• Q: How long does it take to get results from Livestock Disease Kit?
  A: Livestock Disease Kit can give a result within 5-20 mins.
• Q: What type of sample is suitable for Livestock Disease Kit?
  A: Livestock Disease Kit is suitable for blood, serum, and tissue samples.
• Q: Does Livestock Disease Kit require professional equipment?
  A: No, Livestock Disease Kit does not require any professional equipment,can use with our isothermal equipment or pcr machine

The Livestock Disease Kit is a reliable and easy-to-use test kit designed to detect the presence of diseases in livestock, such as cattle, sheep, goats, pigs and horses. The kit has been designed to detect diseases with high accuracy and sensitivity. Also it requires only a small sample volume of 50 μl, making it highly efficient and cost-effective. It is also suitable for a range of canine dog test kit applications, providing fast and accurate results.The reliable detection results make it a reliable and cost-effective solution for farmers and veterinarians looking to diagnose livestock diseases quickly and accurately.

Overview

• Detection kits for Vibrio cholerae
• Available in TaqMan format for analysis
• CE-IVD marked version available for in vitro diagnostic use

Vibrio cholerae is a comma-shaped, gram-negative bacterium. It is the cause of cholera in humans, which affects the upper small intestine. Transmission of the disease is mainly through contaminated food or water. Human subjects affected by cholera exhibit severe watery diarrhea and vomiting, caused by the cholera toxin produced by the bacterium. Many cases of cholera are life-threatening, as diarrhea and associated vomiting can lead to rapid dehydration and electrolyte loss. Even with the extensive research on its epidemiology, cholera still affects over 5 million people per year worldwide.

Vibrio cholerae TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Vibrio cholerae TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM38550 (100 preps)	Cat. TM38510 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
V. cholerae Primer & Probe Mix	280 μL	280 μL
V. cholerae Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Introduction

This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from tissue / blood / body fluid / swab /dry blood spots
Applications	PCR, qPCR, southern bolt and virus detection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Tissue, cell, blood, saliva, swab, blood spot, semen and other clinical samples
Sample amount	Solid tissue : 1-10mg, Anticoagulant blood : 200μl
Yield	1 – 15µg
Elution volume	≥20μl
Time per run	30 – 60 minutes
Liquid carrying volume per column	750µl
Binding yield of column	100µg

Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
• Fast – without separation of leukocytes, organic extraction or ethanol precipitation
• Simple – all nucleic acids can be obtained by direct digestion
• Wide applicability- It can handle various liquid samples, animal tissues and cultured cells

Kit Contents

Contents	IVD3018
Purification Times	100
2ml Collection Tubes	2 x 100
Buffer ATL	60 ml
Buffer AL	60 ml
Buffer GW1	44 ml
Buffer GW2	50 ml
Proteinase K	60 mg
Protease Dissolve Buffer	5 ml
Buffer AE	15 ml

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Experiment Data

Articles

• 【Molecular Cancer 】Malignant clonal evolution drives multiple myeloma cellular ecological diversity and microenvironment reprogramming
• 【BIOSENSORS & BIOELECTRONICS】A multiplexed electrochemical quantitative polymerase chain reaction platform for single-base mutation analysis
• 【eLife】Domain fusion TLR2-4 enhances the autophagy-dependent clearance of Staphylococcus aureus in the genetic engineering goat
• 【TALANTA】Unique dual indexing PCR reduces chimeric contamination and improves mutation detection in cell-free DNA of pregnant women
• 【Microbiology Spectrum】Oral Bacteriome and Mycobiome across Stages of Oral Carcinogenesis | Microbiology Spectrum
• 【Biology of Sex Differences】Integrated chromatin accessibility and DNA methylation analysis to reveal the critical epigenetic modification and regulatory mechanism in gonadal differentiation of the sequentially hermaphroditic fish, Monopterus albus
• 【Cell Reports】Translocational attenuation mediated by the PERK-SRP14 axis is a protective mechanism of unfolded protein response

This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.