NeuroTarget Transfection Agent which is composed of a cation tagged rabies viral glycoprotein (RVG). Experimentally shown to deliver miRNA inside neuronal cell lines containing the nicotinic acetylcholine receptor (nAChR). Highly efficient delivery for the modulation of gene expression and cellular engineering.
Formulation of the rabies viral glycoprotein containing a positively charged tail used for association with nucleic acids. This protein enables the selective delivery of the nucleic acid into neuronal cells.
This peptide is a 29 amino acid fragment derived from rabies virus glycoprotein (RVG). Because neurotropic viruses cross the blood-brain barrier to infect brain cells, the same strategy may be used to enter the central nervous system and deliver siRNA to the brain. This peptide specifically binds to the acetylcholine receptor expressed by neuronal cells.
This product is suitable for rapid RNA extraction from tissue , cells, and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR and so on.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from tissue, cell
Applications
RT-PCR, cDNA synthesis, second generation sequencing
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Sample type
Tissues, cells, lymphocytes and other clinical sample
Sample amount
Cells grown in suspension:3~5 x 106Animal tissue: 10~20mgPlant tissue: ≤100mg
Principle
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase I. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water
Advantages
High purity – OD260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
Economy – less than 50% of the price of Qiagen and other imported products
Automatic – extraction without manual participation, saving time and effort
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at roomtemperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
Document
This product is suitable for rapid RNA extraction from tissue , cells, and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR and so on.
Bis-propargyl-PEG14 is a homobifunctional crosslinker that can participate in Click Chemistry to yield a stable triazole linkage with azide; copper is needed as a catalyst. With PEG chain embedded in the molecule, the hydrophilicity is greatly improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Bis-propargyl-PEG14 is a homobifunctional crosslinker that can participate in Click Chemistry to yield a stable triazole linkage with azide; copper is needed as a catalyst. With PEG chain embedded in the molecule, the hydrophilicity is greatly improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
