NeuroTarget Transfection Agent which is composed of a cation tagged rabies viral glycoprotein (RVG). Experimentally shown to deliver miRNA inside neuronal cell lines containing the nicotinic acetylcholine receptor (nAChR). Highly efficient delivery for the modulation of gene expression and cellular engineering.
Formulation of the rabies viral glycoprotein containing a positively charged tail used for association with nucleic acids. This protein enables the selective delivery of the nucleic acid into neuronal cells.
This peptide is a 29 amino acid fragment derived from rabies virus glycoprotein (RVG). Because neurotropic viruses cross the blood-brain barrier to infect brain cells, the same strategy may be used to enter the central nervous system and deliver siRNA to the brain. This peptide specifically binds to the acetylcholine receptor expressed by neuronal cells.
• NeuroTarget Transfection Agent
Other Products
D6351 MagPure Plant DNA Kit
Product Info
Document
Product Info
Introduction
MagPure Plant DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and tissues.The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minutes. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo.Purified DNA can be used directly for PCR, quantitative PCR, southern blot, hybridization, and transgenosis detection.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 50-100 mg plant, fungal tissue
Applications
PCR, transgene detection, fluorescence quantitative PCR, southern blot, SNP site analysis, etc.
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol toremove salts, and finally DNA was eluted by Elution Buffer.
Advantages
Safe – require no phenol or chloroform extraction
Fast – several samples can be extracted in 60 minutes
High purity – high quality DNA, completely remove inhibitors
Kit Contents
Contents
D635101
D635102
D635103
Purification Times
48 Preps
96 Preps
5 x 96 Preps
MagPure Particles
1.7 ml
4 ml
18 ml
RNase Solution
0.6 ml
1.2 ml
6 ml
Buffer SPL
30 ml
60 ml
300 ml
Buffer PS
10 ml
20 ml
100 ml
Buffer GW1*
26 ml
53 ml
220 ml
Elution Buffer
10 ml
30 ml
120 ml
Storage and Stability
RNase Solution and MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Document
MagPure Plant DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and tissues.The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minutes. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo.Purified DNA can be used directly for PCR, quantitative PCR, southern blot, hybridization, and transgenosis detection.
Nanog is a homeoprotein that functions with pluripotent factors such as Oct4 and Sox2 to maintain embryonic stem cell pluripotency. Expression of this protein has been noted in seminoma, dysgerminoma, embryonal carcinoma, and other undifferentiated germ cell tumors, while nanog expression is absent in normal adult organ tissues. Anti-Nanog may be useful in distinguishing between undifferentiated germ cell tumors and non-germ cell tumors.
[NS1000] FluoroVue™ Nucleic Acid Gel Stain (10,000X), 500 μl
Product Info
Document
Product Info
Description
FluoroVue™ Nucleic Acid Gel Stain (10,000X) is specially designed for in-gel use and is a safer replacement for conventional Ethidium bromide (EtBr), which poses a significant health and safety hazard to its users. It is a fluorescent stain which offers highly sensitive detection of double-stranded or single-stranded DNA and RNA in a convenient manner. FluoroVue™ Nucleic Acid Gel Stain offers high sensitivity that is several times greater than EtBr.
FluoroVue™ Nucleic Acid Gel Stain is compatible with both conventional UV gel-illumination systems as well as harmless long wavelength blue light illumination systems, like B-BOX™. When bound to nucleic acids, FluoroVue™ Nucleic Acid Gel Stain has a fluorescent excitation maximum of 250 and 482 nm, and an emission maximum of 509 nm. Therefore, it can replace EtBr without the need of changing existing lab imaging systems.
Features:
Excellent for in-gel staining
Sensitivity: 0.14 ng (DNA) or 1 ng (total RNA)
A safer alternative to EtBr
Compatibility: suitable to blue or UV light
Increased cloning efficiency (blue light)
Storage
Protected from light 4°C for 12 months -20°C for 24 months
Document
FluoroVue™ Nucleic Acid Gel Stain (10,000X) is specially designed for in-gel use and is a safer replacement for conventional Ethidium bromide (EtBr), which poses a significant health and safety hazard to its users. It is a fluorescent stain which offers highly sensitive detection of double-stranded or single-stranded DNA and RNA in a convenient manner. FluoroVue™ Nucleic Acid Gel Stain offers high sensitivity that is several times greater than EtBr.
FluoroVue™ Nucleic Acid Gel Stain is compatible with both conventional UV gel-illumination systems as well as harmless long wavelength blue light illumination systems, like B-BOX™. When bound to nucleic acids, FluoroVue™ Nucleic Acid Gel Stain has a fluorescent excitation maximum of 250 and 482 nm, and an emission maximum of 509 nm. Therefore, it can replace EtBr without the need of changing existing lab imaging systems.
