NGS Library Circularization Kit (MGI Platform)

Overview

• Detection kits for Clostridium difficile
• Lyophilized format designed for room temperature shipping
• Available in TaqMan format for analysis

Norgen’s Clostridium difficile TaqMan PCR Lyophilized Kit is designed for the detection of Clostridium difficile specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Norgen’s Clostridium difficile TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of Clostridium difficile specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Details

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Product Stability and Storage Conditions

• All kit components should be stored at -20°C upon arrival.
• Once reconstituted, repeated thawing and freezing (>2 times) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
• Each kit is provided with 4 tubes of 2X PCR Master Mix and each tube is enough to run 25 reactions. It is not necessary to reconstitute all Master Mix tubes at once. The Master Mix tubes can be reconstituted as and when needed.
• All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

Component	Cat. TM37150L (100 preps)	Cat. TM37110L (100 preps)
MDx TaqMan 2X PCR Master Mix (Lyo)	4 x 350 μL	–
Clostridium difficile Primer & Probe Mix (Lyo)	280 μL	280 μL
Clostridium difficile Positive Control (Lyo)	150 μL	150 μL
Nuclease-Free Water (Negative Control)	3 x 1.25 mL	1 x 1.25 mL
Product Insert	1	1

Volcano3G® RT-PCR Probe 2x Master Mix contains all components required for general RT-qPCR
– optimized reaction mix for sensitive and reliable results
– engineered, truly thermostable Taq DNA polymerases with reverse transcriptase activity
– fast start function due to a hotstart aptamer formulation which prevents unspecific amplification at lower temperatures (<57°C)
– Pipetting aid in form of a blue dye for better visibility during aliquoting into well-plates
– included lysis function*

* = A sample extraction and sample lysis is not necessary as immediate hot PCR cycling conditions can brake cell- and virus membranes. Please see our published references to this product.

Note: Volcano3G® RT-PCR Probe 2x Master Mix is a unique master mix. If you are using it for the first time, we recommend to design primers to have higher melting/annealing temperatures (above 65°C). Additionally, for assay setup it is advised to perform RT-PCR test reactions with temperature gradients (i.e., for the annealing and extension steps) to identify the most proficient PCR protocol for your needs using our Volcano DNA polymerase products.

For research use and further manufacturing.

In case you are aiming to use our RUO products as components or for your development of e.g. an IVD medical device, please contact us.

Volcano3G® RT-PCR Probe 2x Master Mix contains all components required for general RT-qPCR
– optimized reaction mix for sensitive and reliable results
– engineered, truly thermostable Taq DNA polymerases with reverse transcriptase activity
– fast start function due to a hotstart aptamer formulation which prevents unspecific amplification at lower temperatures (

K-LARGE

SKU: 700004309

50 Assays per kit

Content:	50 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 1 year under recommended storage conditions

Analyte:	Ammonia, L-Arginine, Nitrogen, Urea, YAN
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Decrease
Linear Range:	1.0 to 35 mg of L-arginine, 0.2 to 7.0 μg of ammonia and 0.3 to 14 µg of urea per assay
Limit of Detection:	0.07 mg/L (ammonia), 0.13 mg/L (urea), 0.37 mg/L (L-arginine)
Reaction Time (min):	~ 20 min [ammonia (2 min), urea (6 min), L-arginine (7 min)]
Application examples:	Grape juice, wine must, wine and other materials (e.g. biological cultures, samples, etc.).
Method recognition:	Improved method

The L-Arginine/Urea/Ammonia test kit is specific and a rapid measurement and analysis of L-arginine, urea and ammonia in grape juice/must and wine.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuant^TM  Wave Spectrophotometer (D-MQWAVE).

Display all of our nitrogen assay kit products.

Advantages

• Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4^oC.
• Improved assay format 
• Very rapid reactions due to use of uninhibited glutamate dehydrogenase 
• All enzymes supplied as stabilised suspensions 
• Very competitive price (cost per test) 
• All reagents stable for > 2 years after preparation 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included

The L-Arginine/Urea/Ammonia test kit is specific and a rapid measurement and analysis of L-arginine, urea and ammonia in grape juice/must and wine.