NGS Library Quantification Standards With PCR Primers (Ion Torrent Platform)
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The NGS Library Quantification Standards with PCR Primers (for Ion Torrent platform) were developed for quantifying the library concentration for ion torrent sequencing platform. Quantification of the library of the fully ligated libraries is important for the quality of the sequencing outcome. Optimal library concentration can increase sequencing yield. Poor library concentration results in bad emPCR, which can lead to low sequencing capacity.
Detail
The NGS Library Quantification Standards with PCR Primers (for Ion Torrent platform) were developed for quantifying the library concentration for ion torrent sequencing platform. Quantification of the library of the fully ligated libraries is important for the quality of the sequencing outcome. Optimal library concentration can increase sequencing yield. Poor library concentration results in bad emPCR, which can lead to low sequencing capacity.
QPCR is the best method for library quantification. Our reagent only amplifies library molecules that will be used for subsequent emPCR, and is optimized for amplification of various samples. Our reagent is compatible with commercial SYBR Green based QPCR reagents. Quantification of library concentration is achieved by comparison with a standard curve generated from DNA Standards.
The kit comprises DNA Standards (six 10-fold dilutions) and a primer mix.
NGS Library Quantification Standards with PCR Primers (Ion Torrent platform): real time quantitative PCR curve of the standards.
Other Products
D5131 MagPure Bacterial DNA Kit
Product Info
Document
Product Info
Introduction
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, plasmid) can be purified from bacterial cultures.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from bacterial culture
Applications
PCR, southern blot and enzyme digestion, etc.
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Gram negative or positive bacteria
Sample amount
Bacterial culture: 0.5-1.5ml
Elution volume
≥60μl
Time per run
≤60 minutes
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
Fast – several samples can be extracted in 60 minutes (after digestion)
High purity – unique adsorbent can completely remove inhibitory factors
High recovery – DNA can be recovered at the level of PG
Kit Contents
Contents
D513101
D513102
D513103
Purification Times
48 Preps
96 Preps
480 Preps
MagPure Particles
1.2 ml
2.5 ml
11 ml
Lysozyme
60 mg
120 mg
600 mg
Proteinase K
24 mg
50 mg
220 mg
Protease Dissolve Buffer
3 ml
6 ml
30 ml
Buffer SDS
1.5 ml
3 ml
15 ml
Buffer MLA
30 ml
60 ml
300 ml
Buffer GW1*
13 ml
44 ml
110 ml
Buffer TE
30 ml
40 ml
200 ml
Storage and Stability
MagPure Particles, Lysozyme and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Document
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, plasmid) can be purified from bacterial cultures.
Opentrons OT-2 Single-Channel Electronic Pipettes automate liquid handling and pipetting for most daily wetlab applications. Best for low to medium throughput workflows with high precision.
Document
Opentrons OT-2 Single-Channel Electronic Pipettes automate liquid handling and pipetting for most daily wetlab applications. Best for low to medium throughput workflows with high precision.
1. The instantaneous centrifugal function rises to the maximum speed for about 6 seconds, allowing for rapid detachment of hanging droplets from the wall;
2. Faster acceleration and deceleration rate, required to complete the experiment in a shorter time;
3. Door cover protection, overspeed and imbalance detection system, capable of real-time monitoring of the centrifugal process, ensuring the safe operation of the instrument; When the operation ends, an error occurs, or an imbalance occurs, the sound signal prompts, and the operation stops at the same time. The LCD displays the result code
Host Parameters
Product model
Mini-3
Input power supply
DC24V/2.75A
Input power
55W
Motor/Drive Method
DC24V brushless DC variable frequency motor
Display method
High brightness LCD screen
Effective centrifugal time
1-99min.1-59sec
speed
300~3000±15rpm
Speed step increase
10rpm
Maximum capacity
2 *96 well PCR plates/ELISA plates
MAX. RCF
608 xg
Misoperation/alarm failure
Sound prompt+display code
weight
3.9Kg
Noise
60dB (A)
Fastest acceleration time
<6s
Fastest deceleration time
<5s
Permissible ambient temperature/relative temperature
+5-40°C/80%
Document
The micro porous plate centrifuge adopts international advanced design concepts and manufacturing technology, with a smooth and beautiful appearance, compact and stable structure, adhering to the advantages of high universality and easy operation. It also has the functional characteristics of “smooth start” and “smooth braking”; This micro porous plate centrifuge is very suitable for 96 or 384 well and small capacity micro porous plates, as well as various standard PCR micro porous plates with or without skirts .
