Norgen’s Nuclease-Free Water is free of all DNases, RNases and nucleic acids and is suitable for all molecular biology applications requiring nuclease-free water including PCR, RT-PCR and real-time PCR. Our water is prepared without the use of DEPC, and is ready to use without further treatment.
Applications For use in any molecular biology application
Storage Conditions This kit is stable for 2 years after the date of shipment.
Precautions and Disclaimers This product is designed for research purposes only. It is not intended for human or diagnostic use.
Other Products
microScript microRNA cDNA Synthesis Kit
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Overview
Convenient
One cDNA Synthesis, Multiple microRNAs and microRNA-targets analyzed
Time Savings
Cost Efficient
High Sensitivity and Yield
Robust Enzyme
Available in 12 or 50 reaction size
Norgen’s microScript microRNA cDNA Synthesis Kit is an all-in-one, ready-to-use product for the reverse transcription of microRNA from either Total RNA preparations or enriched microRNA preparations. The kit contains the 2x Reaction Mix and the microScript microRNA Enzyme Mix. The kit utilizes Norgen’s microScript Reverse Transcriptase, a mutant version of Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase. It has reduced RNase H activity and increased thermal stability.
The workflow of Norgen’s microScript microRNA cDNA Synthesis Kit involves a simple, single-tube set-up by the mixing of 2x Reaction Mix, Enzyme Mix and the RNA template. The reaction can then be carried out in a thermocycler. A poly (A) tail is first added to the RNA template, followed by cDNA synthesis using an adapter primer. In addition to the ease-of-use, the single-tube set-up provides superb consistency and sensitivity. The cDNA could be used in a PCR or qPCR amplification using a Universal PCR Reverse Primer and the forward primer that contains the sequence of the microRNA of interest. A single cDNA preparation could be used for PCR amplification of a number of different microRNAs. In addition, the cDNA preparation could be used for PCR or qPCR detection (using gene-specific forward and reverse primers) of mRNA or large RNA if total RNA preparation was the starting template. This could allow for parallel evaluation of expression level of microRNAs and microRNA-targets.
Storage Conditions and Product Stability Norgen’s microScript microRNA cDNA Synthesis Kit components should be stored at -20°C. These reagents should remain stable for at least 1 year in their unopened containers.
Propargyl-PEG8-t-butyl ester is a PEG reagent with an alkyne group and a t-butyl group. The alkyne group enables the formation of triazole linkage with azide-bearing compounds or biomolecules; copper is required as a catalyst. The t-butyl group can be hydrolyzed under acidic conditions. The hydrophicility of the molecule is improved by having 8 units of PEG. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Propargyl-PEG8-t-butyl ester is a PEG reagent with an alkyne group and a t-butyl group. The alkyne group enables the formation of triazole linkage with azide-bearing compounds or biomolecules; copper is required as a catalyst. The t-butyl group can be hydrolyzed under acidic conditions. The hydrophicility of the molecule is improved by having 8 units of PEG. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Human Enterovirus (HEV) TaqMan RT-PCR Detection Kits
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Detection kits for HEV
CE-IVD marked version available for in vitro diagnostic use
Available in TaqMan format for analysis
The Enteroviruses are a genus of the family Picornaviridae, and are a large and diverse group of small RNA viruses characterized by a ssRNA molecule of positive sense. To date, over 70 serotypes of Enterovirus have been isolated from man. The human enteroviruses include poliovirus, Coxsackie A and Coxsackie B viruses, enterocytopathic human orphan (ECHO) viruses and other enteroviruses. Enteroviruses infect millions of people worldwide each year, and the infection can result in a wide variety of symptoms depending on the serotype of the enterovirus. Symptoms of infection may include, but are not limited to, mild respiratory illness (common cold), hand, foot and mouth disease, Rubelliform rashes, acute hemorrhagic conjunctivitis, aseptic meningitis, myocarditis, and neonatal infections.
HEV TaqMan RT-PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
HEV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
