The oasig freeze drying process stabilises all of the active components allowing these reagents to be shipped and stored at room temperature. This hugely simplifies the logistics of purchasing, shipping and using the technology. Whether you are in a sophisticated laboratory in Texas or a mobile field hospital in Timbuktu we can supply complete qPCR kit and reagent packages to your door quickly and cheaply via standard shipping methods without the need for dry ice or a cold chain of any sort.
The performance of the reagents is second to none. We are confident that you will find excellent data quality and even see an improvement in data quality versus many traditional frozen master mixes.
No cold shipping required. Our unique, oasig Lyophilised qPCR Master Mix in a 150 reaction pack. Perfect to accompany any genesig kit for a DNA target.
Primerdesign real-time PCR reagents are manufactured to the highest standards within our ISO9001:2008 and ISO13485:2012 certified quality management laboratory environment.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Document
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
150 reactions
It is suitable for the extraction and purification of viral nucleic acid and free nucleic acid. It can bind the nucleic acids in solution through hydrophobic, hydrogen bonding and electrostatic interaction under high salt conditions, without binding with other impurities (such as proteins), and quickly separate nucleic acids from biological samples. The operation is safe and simple, which is very conducive to the automatic and high-throughput extraction of short fragments or low abundance nucleic acids.
Product characteristics
Super paramagnetism and high magnetic response, saving operation time and increasing sample recovery efficiency.
Good suspension performance, dispersion and resuspension, which is conducive to efficient nucleic acid binding and recovery.
Good physical and chemical stability to ensure repeatability.
Product information
Document
Note: Price not include shipment & duty, contact us to get full quote.
Magnetic beads are specially designed for nucleic acid extraction and purification, with good suspension performance. The surface is modified with a large number of carboxyl groups, which can bind the nucleic acid in the sample through hydrophobic, hydrogen bonding and electrostatic interaction under high salt and low pH conditions, without binding with other impurities (such as proteins), and quickly separate nucleic acid from biological samples. The operation is safe and simple. It is beneficial to the automation and high throughput extraction of nucleic acid.
The 16S V2-V3 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V2-V3 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V2-V3 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V2-V3 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
Storage Conditions and Product Stability Norgen’s 16S V2-V3 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
