The oasig freeze drying process stabilises all of the active components allowing these reagents to be shipped and stored at room temperature. This hugely simplifies the logistics of purchasing, shipping and using the technology. Whether you are in a sophisticated laboratory in Texas or a mobile field hospital in Timbuktu we can supply complete qPCR kit and reagent packages to your door quickly and cheaply via standard shipping methods without the need for dry ice or a cold chain of any sort.
The performance of the reagents is second to none. We are confident that you will find excellent data quality and even see an improvement in data quality versus many traditional frozen master mixes.
No cold shipping required. Our unique, oasig Lyophilised qPCR Master Mix in a 150 reaction pack. Perfect to accompany any genesig kit for a DNA target.
Primerdesign real-time PCR reagents are manufactured to the highest standards within our ISO9001:2008 and ISO13485:2012 certified quality management laboratory environment.
Other Products
HCM114 Bile Esculin Azide Agar
Product Info
Document
Product Info
Introduction
Intended Use
For the selective separation and enumeration of enterococci in food and water.
Principle and Interpretation
Tryptone and peptone are the sources of nitrogen and essential growth factors. Yeast extract acts as well nitrogenous compounds and additionally the vitamin B12 complex. Sodium azide acts largely inhibits the growth of gram-negative bacteria while sparing enterococci, staphylococci and streptococci. Ox bile inhibits most gram positives but not enterococci. Enterococci hydrolyse esculin to esculetin and dextrose, which reacts with ferric citrate producing a brownish black precipitate around the colonies. Tolerance to bile and the ability to hydrolyze esculin is the traditional and reliable test for the identification of enterococci. (4). Sodium chloride maintains the osmotic balance of the medium and Agar is the solidifying agent.
Formulation
Ingredients
/liter
Tryptone
17.0g
Ox bile
10.0g
Yeast extract
5.0g
Sodium chloride
5.0g
Peptone
3.0g
aesculin
1.0g
Ferric ammonium citrate
0.5g
Sodium azide
0.15g
Agar
15.0g
pH 7.1±0.1 at 25°C
Preparation
Weigh 56.6g of dry powder of this product, add 1 L of distilled water or deionized water, stir, heat and boil until completely dissolved, and sterilize at 121℃ for 15 min.
Quality Control
Cultural characteristics observed after incubation at 35-37°C for 20-24hours.
Quality control strains
Growth
Colony color
Enterococcus faecalis ATCC29212
PR≥0.7
Brown-black halo
Escherichia coli ATCC25922
inhibited
Absence of brown-black halo
Sorage and Shelf Life
Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Document
Intended Use For the selective separation and enumeration of enterococci in food and water. Principle and Interpretation Tryptone and peptone are the sources of nitrogen and essential grow……
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from small volume of blood, tissue and dry blood spots.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 1-10μl blood, <10mg tissue, urine, blood stain, seminal stain
Applications
PCR, southern bolt and virus detection, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Animal tissues, blood stain, urine, seminal stain and various forensic samples
Sample amount
Blood:1-100μl, Tissue:<10mg
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column
Principle
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA).
Advantages
Fast – several samples can be extracted in 20 minutes (after digestion)
High purity – purified DNA can be directly used in various downstream applications
High recovery – DNA can be recovered at the level of PG
Good repeatability – silica technology can obtain ideal results every time
Kit Contents
Contents
D312502
D312503
Purification Times
50 Preps
250 Preps
Buffer ATL
15 ml
60 ml
Buffer AL
15 ml
60 ml
Buffer GW1*
22 ml
66 ml
Buffer GW2*
20 ml
2 x 50 ml
Carrier RNA
310 μg
2 x 310 µg
Proteinase K
24 mg
120 mg
Protease Dissolve Buffer
1.8 ml
10 ml
Buffer AE
15 ml
60 ml
HiPure DNA Mini Columns I
50
2 x 125
2 ml Collection Tubes
100
5 x 100
Storage and Stability
Carrier RNA and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.
Experiment Data
Document
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from small volume of blood, tissue and dry blood spots.
Tetra(3-methoxy-N-(PEG2-prop-2-ynyl)propanamide) Methane is a 4-branched molecule with propargyl groups that can be linked to azide-containing biomolecules via Click Chemistry. The hydrophilic PEG linker increases the water solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Tetra(3-methoxy-N-(PEG2-prop-2-ynyl)propanamide) Methane is a 4-branched molecule with propargyl groups that can be linked to azide-containing biomolecules via Click Chemistry. The hydrophilic PEG linker increases the water solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
