Okadaic Acid (DSP) ELISA Kit

Competitive ELISA for the quantitative analysis of Okadaic Acid (DSP)
Format: 96-well microtiter plate (12 test strips of 8 wells)
Okadaic acid is a potent neurotoxin and phosphatase inhibitor from dinoflagellate black sponges that are associated with seafood poisonings.

Description

Okadaic Acid (OA) is a one of the diarrhetic shellfish poisons (DSP) produced by dinoflagellate genera Dinophysis and Prorocentrum. There are several chemically different toxins associated with DSP.

They are lipophilic and polyether compounds and can be divided into three main groups:

Acidic toxins
Neutral toxins
Other toxins 2 Contamination of shellfish with OA has been associated with harmful algae blooms throughout the world.

In humans, DSP causes dose-dependent symptoms of diarrhea, nausea, and vomiting. The action levels established by the FDA for OA is 200ppb. The EU has established a level of 160 ppb of OA or its equivalent.

The Attogene Okadaic acid ELISA kit enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organizations to detect OA in food, feed, fish, and environmental samples of concern.

Okadaic acid is the causative agent of Diarrhetic Shellfish Poisoning (DSP).

FDA and EPA Safety Levels in Regulations and Guidance – 0.16 mg/kg for Clams, mussels, oysters, and whole and roe-on scallops, fresh, frozen, or canned. – National Shellfish Sanitation Program Guide for the Control of Molluscan Shellfish.

Cat. #	Name	Elution Volume	Plasma/Serum	Urine	Cell-Culture Media
55000	Plasma/Serum RNA Purification Mini Kit	10 – 25 µL	50 µL – 1 mL	250 µL – 1 mL	5 – 10 mL
35300	Total RNA Purification Micro Kit	20 – 50 µL	1 – 4 mL	2 – 10 mL	10 – 20 mL
17200	Total RNA Purification Kit	50 – 100 µL	4 – 10 mL	11 – 30 mL	20 – 35 mL

Kit Specifications
Maximum Binding Capacity	Up to 50 μg RNA
Maximum Loading Volume	650 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material
Animal Cells	3 x 10⁶ cells
Animal Tissues	10 mg (for most tissues*)
Blood	100 μL
Plasma/Serum	200 μL
Bacteria	1 x 10⁹ cells
Yeast	1 x 10⁸ cells
Fungi	50 mg
Plant Tissues	50 mg
Time to Complete 10 Purifications	20 minutes
Average Yield
HeLa Cells (1 x 10⁶ cells)	15 μg
E. coli (1 x 10⁹ cells)	50 μg

Component	Cat. 17200 (50 preps)	Cat. 37500 (100 preps)	Cat. 17250 (250 preps)	Cat. 17270 (500 preps)	Cat. 24300 (192 preps)	Cat. 24370 (576 preps)	Cat. 24350 (192 preps)	Cat. 24380 (576 preps)
Buffer RL	40 mL	2 x 40 mL	175 mL	350 mL	2 x 40 mL	350 mL	2 x 40 mL	350 mL
Wash Solution A	38 mL	2 x 38 mL	148 mL	1 x 148 mL 1 x 74 mL	2 x 38 mL	1 x 74 mL 1 x 148 mL	2 x 38 mL	1 x 74 mL 1 x 148 mL
Elution Solution A	6 mL	2 x 6 mL	30 mL	60 mL	2 x 20 mL	60 mL	2 x 20 mL	60 mL
Mini Spin Columns	50	100	250	500	–	–	–	–
96-Well Isolation Plate	–	–	–	–	2	6	–	–
96-Well Isolation Plate (Deep Well)	–	–	–	–	–	–	2	6
Adhesive Tape	–	–	–	–	4	12	4	12
Collection Tubes	50	100	250	500	–	–
96-Well Collection Plate	–	–	–	–	2	6	–	–
96-Well Collection Plate (Deep Well)	–	–	–	–	–	–	2	6
Elution Tubes (1.7 mL)	50	100	250	500	–	–
96-Well Elution Plate	–	–	–	–	2	6	–	–
96-Well Elution Plate (Deep Well)	–	–	–	–	–	–	2	6
Product Insert	1	1	1	1	1	1	1	1

Features	Specifications
Main Functions	Recover DNA fragments >100bp from agarose gel(<0.2g), purification of DNA from PCR or enzymatic reaction solution solution
Applications	PCR, NGS, labeling, ligation and enzyme digestion, etc.
Purification method	Micro spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Agarose gel, PCR products, enzyme products
Sample amount	Agarose gel: ≤200mgDNA products: ≤300µl (≤5µg)
Recovery	100-5000bp：85%, 5-10kb：60-70%
Elution volume	≥10μl
Time per run	≤30 minutes（1-24 samples）
Liquid carrying volume per column	800µl
Binding yield of column	20µg

Contents	D211002	D211003
Purification Times	100 Preps	250 Preps
Buffer GDP	60 ml	125 ml
Buffer DW1	40 ml	90 ml
Buffer DW2	20 ml	50 ml
Elution Buffer	20 ml	30 ml
HiPure DNA Micro Columns	100	250
2 ml Collection Tubes	100	250

Okadaic Acid (DSP) ELISA Kit

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