Competitive ELISA for the quantitative analysis of Okadaic Acid (DSP) Format: 96-well microtiter plate (12 test strips of 8 wells) Okadaic acid is a potent neurotoxin and phosphatase inhibitor from dinoflagellate black sponges that are associated with seafood poisonings.
Detail
Competitive ELISA for the quantitative analysis of Okadaic Acid (DSP)
Format: 96-well microtiter plate (12 test strips of 8 wells)
Okadaic acid is a potent neurotoxin and phosphatase inhibitor from dinoflagellate black sponges that are associated with seafood poisonings.
Okadaic Acid (OA) is a one of the diarrhetic shellfish poisons (DSP) produced by dinoflagellate genera Dinophysis and Prorocentrum. There are several chemically different toxins associated with DSP.
They are lipophilic and polyether compounds and can be divided into three main groups:
Acidic toxins
Neutral toxins
Other toxins 2 Contamination of shellfish with OA has been associated with harmful algae blooms throughout the world.
In humans, DSP causes dose-dependent symptoms of diarrhea, nausea, and vomiting. The action levels established by the FDA for OA is 200ppb. The EU has established a level of 160 ppb of OA or its equivalent.
The Attogene Okadaic acid ELISA kit enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organizations to detect OA in food, feed, fish, and environmental samples of concern.
Okadaic acid is the causative agent of Diarrhetic Shellfish Poisoning (DSP).
FDA and EPA Safety Levels in Regulations and Guidance – 0.16 mg/kg for Clams, mussels, oysters, and whole and roe-on scallops, fresh, frozen, or canned. – National Shellfish Sanitation Program Guide for the Control of Molluscan Shellfish.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Nitrogen, Primary Amino Nitrogen, YAN
Assay Format:
Spectrophotometer, Microplate, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
0.2 to 10 µg of amino nitrogen per assay
Limit of Detection:
2.59 mg N/L
Reaction Time (min):
~ 15 min
Application examples:
Grape juice, must, wine and other materials.
Method recognition:
Novel method
The Primary Amino Nitrogen (PANOPA) Assay Kit is suitable for the measurement and analysis of primary amino nitrogen in grape juice/must and wine.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
SMOBIO’s B-BOX™ is a long wavelength, blue light LED epi-illuminator. It is compact in design and robust in structure. The B-BOX™ epi-illuminator provides an unprecedented level of safety for its user due to its non-UV light source and a low operating voltage of only 12 Volts, as well as its capability working with non-carcinogenic DNA/ protein dye. The B-BOX™ comes with high quality non-flickering LED light that is highly sensitive for DNA and protein dye. B-BOX™ can greatly ease the routine chore of gel extraction, enabling easy visualization and gel cutting even in bright ambient light. The multi-angle filter plate (Cat. No. VE0102) provides optimal angles for gel cutting, visualization, and documentation. The B-BOX™ also comes with amber colored filter goggles (Cat. No. VE0103) for gel cutting and visualization. The Phox™ Photobox (Cat. No. VE0104) provides a mini darkroom environment for images taken with any smartphone. Finally, a built-in barrier system around the working area helps facilitate cleaning.
Features
Improved cloning efficiency
Compact, lightweight, and portable (less than 1 kg in weight)
Safety features include : 470 nm long wavelength, without any UV radiation hazard to its user
Compatible with non-carcinogenic, non-ethidium bromide DNA staining dye
User friendly: Samples are easy to visualize (when using the filter plate or goggles)
LED light source lasts up to 50,000 hours
Superior detection sensitivity: ≤ 0.04 ng of DNA when using FluoroStain™ DNA Fluorescent Staining Dye, ≤3 ng of protein when using FluoroStain™ Protein Fluorescent Staining Dye (as sensitive as silver stain)
Adjustable and removable filter plate allows for gel cutting, visualization, and documentation
Built-in barrier design, for easy clean up
Visible in bright ambient light
Emphasizes minimal power reliance, low heat generation, with its own built-in heat sink
Document
SMOBIO’s B-BOX™ is a long wavelength, blue light LED epi-illuminator. It is compact in design and robust in structure. The B-BOX™ epi-illuminator provides an unprecedented level of safety for its user due to its non-UV light source and a low operating voltage of only 12 Volts, as well as its capability working with non-carcinogenic DNA/ protein dye. The B-BOX™ comes with high quality non-flickering LED light that is highly sensitive for DNA and protein dye. B-BOX™ can greatly ease the routine chore of gel extraction, enabling easy visualization and gel cutting even in bright ambient light. The multi-angle filter plate (Cat. No. VE0102) provides optimal angles for gel cutting, visualization, and documentation. The B-BOX™ also comes with amber colored filter goggles (Cat. No. VE0103) for gel cutting and visualization. The Phox™ Photobox (Cat. No. VE0104) provides a mini darkroom environment for images taken with any smartphone. Finally, a built-in barrier system around the working area helps facilitate cleaning.
Preservation and isolation of both cf-DNA and cf-RNA from a single tube
Fixative-free preservative, no cross-linking of DNA
Preserve cf-DNA/ct-DNA for 30 days at ambient temperature and for up to 8 days at 37°C
Preserve cf-RNA for 30 days at ambient temperature
Preserve Circulating Tumour Cells (CTCs) for 14 days at ambient temperature
No plasma volume loss after shipping/transportation
Prevent hemolysis allowing better separation of plasma
Prevent apoptosis of blood cells and fragmentation of genomic DNA
Produce high quality/quantity of plasma cf-DNA/ct-DNA/cf-RNA
Vacuumed to draw 8.7 mL of blood in 10 mL tubes
The procedure for plasma isolation using this kit is compliant with ISO 20186-3:2019
Norgen’s cf-DNA/cf-RNA Preservative Tubes are closed, evacuated plastic tubes for the collection and the preservation of cf-DNA, circulating tumor DNA, cf-RNA and circulating tumor cells in human whole blood samples during storage and shipping. Using these tubes in combination with Norgen’s Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen’s Plasma/Serum RNA Purification Kits enables the purification and concentration of an inhibitor-free cf-DNA/ct-DNA/cf-RNA in a very small elution volume. Plasma recovered from Norgen’s cf-DNA/cf-RNA Preservative Tubes is also compatible with any other cf-DNA and/or cf-RNA purification method. The purified cf-DNA, ct-DNA and cf-RNA are compatible with any downstream application assays, including PCR, qPCR, rt-qPCR, methylation-sensitive PCR, Southern Blot analysis, gene expression analysis, microarrays and NGS.
This product is for research use only and not for use in diagnostic procedures.
Performance
Norgen’s cf-DNA/cf-RNA Preservative Tubes abridge the collection/processing of whole blood for the subsequent purification of cf-DNA and/or cf-RNA. The cf-DNA/cf-RNA Preservative Tubes preserve and inhibit the programmed cell-death (apoptosis) of the blood, hence preventing the release of intracellular DNA/RNA into plasma. Whole blood samples can be shipped and stored at room temperature for the subsequent processing of plasma. The cf/ct DNA and cf-RNA levels are stable for up to 30 days at room temperature. The cf-DNA is also stable for up to 8 days at 37°C. When blood collected on Norgen’s cf-DNA/cf-RNA tubes is processed for plasma recovery no buffy coat will be generated and the Circulating tumor cells (CTCs) will be located at the bottom of the tube with the blood sediment. CTCs are stable for 14 days at room temperature where it can be extracted from the blood sediment or from whole preserved blood for cell sorting or for cf/DNA or cf-RNA purification.
Plasma samples produced from the whole blood sample collected into Norgen’s cf-DNA/cf-RNA Preservative Tubes and purified using Norgen’s Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen’s Plasma/Serum RNA Purification Kits yield concentrated, high-quality and inhibitor-free cf-DNA/ct-DNA or cf-RNA for any downstream application.
Workflow
NOTE: This product is for research use only. For the CE marked version, see Cat. Dx63950
Up to 30 days at room temperature (15-25°C) Up to 8 days at 37°C
Length of cf-RNA Preservation
Up to 30 days at room temperature (15-25°C)
Length of CTCs Preservation
Up to 14 days at room temperature (15-25°C)
Storage Conditions and Product Stability
When stored at 15-30°C, unfilled Norgen cf-DNA/cf-RNA Preservative Tubes are stable through the expiration date. See tube label for expiry date.
Short-term storage/shipping at -20°C to 37°C for up to 10 days is acceptable for unfilled Norgen cf-DNA/cf-RNA Preservative Tubes.
Do not freeze unfilled Norgen cf-DNA/cf-RNA Preservative Tubes for long term storage over 10 days. Freezing unfilled tubes may lead to loss of vacuum and precipitation may occur.
Cell-free DNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes is stable for up to 30 days when stored at room temperature (15-25°C)
Cell-free DNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes is stable for up to 8 days when stored at 37ºC.
Cell-free RNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes is stable for up to 30 days when stored at room temperature (15-25°C)
Circulating tumor cells (CTCs) in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes are stable for up to 14 days at room temperature (15-25ºC).
