Opentrons Flex 8-Channel pipettes enable highly accurate pipetting, optimized for automation.
Flex pipettes use air displacement technology to offer highly accurate pipetting with pipette volume ranges from 1 to 50 µL and 5 to 1000 μL. Smart sensors support automatic calibration, real-time positioning, and error detection. Choose the pipettes that fit your workflow — the gantry supports any two Flex 1-Channel or 8-Channel Pipettes — and swap them out yourself when your workflow changes.
Opentrons Flex 8-Channel pipettes enable highly accurate pipetting, optimized for automation.
Flex pipettes use air displacement technology to offer highly accurate pipetting with pipette volume ranges from 1 to 50 µL and 5 to 1000 μL. Smart sensors support automatic calibration, real-time positioning, and error detection. Choose the pipettes that fit your workflow — the gantry supports any two Flex 1-Channel or 8-Channel Pipettes — and swap them out yourself when your workflow changes.
N-(Boc-PEG1)-N-bis(PEG2-propargyl) is a click chemistry branched linker. The propargyl groups can react with azide-bearing molecule via copper catalyzed Click Chemistry . The Boc group can be deprotected under acidic conditions to release amine group. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
N-(Boc-PEG1)-N-bis(PEG2-propargyl) is a click chemistry branched linker. The propargyl groups can react with azide-bearing molecule via copper catalyzed Click Chemistry . The Boc group can be deprotected under acidic conditions to release amine group. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Intended Use
For the enumeration of enterococci in water and other liquids by the membrane filtration technique.
Principle and Interpretation
The growth of the entire accompanying Gram-negative microbial flora is inhibited by sodium azide. Enterococci reduce 2,3,5-Triphenyl tetrazolium chloride (TTC) to give a red formazan inside the bacterial cell, their colonies are thus red. Nitrogen, minerals, and amino acids are provided by the tryptose whilst yeast extract supplies vitamins. Glucose acts as the carbon source, dipotassium phosphate buffers the medium, and agar-agar is the solidifying agent.
Formulation
| Ingredients | /liter |
| Tryptose | 20.0g |
| Yeast extract | 5.0g |
| Glucose | 2.0g |
| Disodium hydrogen phosphate | 4.0g |
| Sodium azide | 0.4g |
| 2,3,5-Triphenyl Tetrazolium chloride | 0.1g |
| Agar | 10.0g |
| pH 7.2±0.1 at 25°C | |
Preparation
Dissolve 41.5 g in 1 L of purified water. Heat in boiling water and agitate frequently until completely dissolved. Sterilize by further heat for 20 minutes in the boiling water bath.
Quality Control
Cultural characteristics observed after incubation at 34-38°C for 40-48hours
| Quality control strains | Growth | Colony color |
| Enterococcus faecalis ATCC29212 | Good growth,PR≥0.5 | deep red coloured colonies |
| Escherichia coli ATCC25922 | Total inhibition | – |
Sorage and Shelf Life
Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Intended Use For the enumeration of enterococci in water and other liquids by the membrane filtration technique. Principle and Interpretation The growth of the entire accompanying Gram-neg……
