50 µL filtered pipette tips for the Opentrons Flex, provided sterile in racks or blister packs (refills). Tips are clear, non-conducting polypropylene tips, free of human DNA, PCR inhibitors, RNase/DNase, pyrogens, and endotoxins. Opentrons Flex Tips and Racks are autoclavable.
50 µL filtered pipette tips for the Opentrons Flex, provided sterile in racks or blister packs (refills). Tips are clear, non-conducting polypropylene tips, free of human DNA, PCR inhibitors, RNase/DNase, pyrogens, and endotoxins. Opentrons Flex Tips and Racks are autoclavable.
Propargyl-PEG10-alcohol is a heterobifunctional PEG linker which enables Click Chemistry reactions with azide-bearing biomolecules; copper catalyst is needed. The hydrophilic PEG units increase aqueous solubility. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG10-alcohol is a heterobifunctional PEG linker which enables Click Chemistry reactions with azide-bearing biomolecules; copper catalyst is needed. The hydrophilic PEG units increase aqueous solubility. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.
The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.
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| Kit Specifications – 96-well | |
| Binding Capacity Per Well | 15 μg |
| Maximum Loading Volume Per Well | 500 μL |
| Size of DNA Purified | 100 – 15,000 bp |
| Average DNA Recovery | > 90% |
| Average Primer Reoval | > 90% |
| Minimum Elution Volume | 50 μL |
| Time to Complete 96 Purifications | 20 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 14400 (50 preps) | Cat. 45700 (250 preps) | Cat. 24800 (192 preps) |
|---|---|---|---|
| Binding Buffer C | 30 mL | 5 x 30 mL | 3 x 30 mL |
| Wash Solution A | 12 mL | 2 x 20 mL | 2 x 38 mL |
| Elution Buffer B | 8 mL | 2 x 30 mL | 2 x 15 mL |
| Spin Columns | 50 | 250 | – |
| Collection Tubes | 50 | 250 | – |
| 96-Well Plate | – | – | 2 |
| Adhesive Tape | – | – | 4 |
| 96-Well Collection Plate | – | – | 2 |
| Elution Tubes (1.7 mL) | 50 | 250 | – |
| 96-Well Elution Plate | – | – | 2 |
| Product Insert | 1 | 1 | 2 |
Permagen’s 5 mL Centrifuge Tube Magnetic rack is designed for magnetic bead separations from up to eight, 5 mL Centrifuge tubes
Accommodates any common 5 mL Centrifuge Tubes
Rapid bead separations
Beads will be pulled to back wall allowing easy aspiration and tip tracking down the front wall of the tubes without disturbing bead pellet
Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic beads
MSR8X5
Maximum Volume – 5.0 mL
Minimum Volume – 1.0 mL
Permagen’s 5 mL Centrifuge Tube Magnetic rack is designed for magnetic bead separations from up to eight, 5 mL Centrifuge tubes
