Opentrons Flex NGS Workstation

Overview

• Detection kits for Chlamydia/Neisseria gonorrhoeae
• Available in TaqMan format for analysis

Chlamydia trachomatis, the causative agent of Chlamydia, is a Gram negative bacteria. Transmission of the bacteria occurs via contact with infected bodily fluids which then infect mucosal membranes. It can be transmitted from mother to child during pregnancy and infect the eyes causing conjunctivitis. The genital infection causes urethritis, epididymitis and prostatitis in males and Pelvic Inflammatory Disease (PID) in females with an increased risk of contracting HIV. The infection can be treated with a course of antibiotics. Sexually transmitted infections in females are most often asymptomatic, but can be noticeable in chronic pain of the pelvic region, vaginal bleeding and painful urination. Infection of the ovaries, fallopian tubes or uterus causes Pelvic Inflammatory Disease (PID) which can lead to difficulties in conceiving, increased risk of ectopic pregnancy or infertility. Infections in males are more likely to be symptomatic, causing painful urination, discharge from the penis and swollen testicles and may eventually cause infertility if left untreated.

Neisseria gonorrhoeae is a Gram-negative coccus of the Neisseria genus. N. gonorrhoeae is usually seen in pairs infecting human cells. It has a circular DNA genome of approximately 1Mbp encoding over 2000 genes. N. gonorrhoeae is transmitted by sexual contact and usually causes infection in cells of the mucous membrane of the male urethra or the endocervix and urethra in females. During infection, polysaccharides are released from the bacteria that stimulate host cell production of tumour necrosis factors that cause an inflammatory response. There is no vaccine against N. gonorrhoeae infection and antibiotic resistance is beginning to increase, therefore treatment includes a course of antibiotics that will be effective against resistant strains. Complications in males caused by the infection can result in prostatitis or orchitis if the bacteria spread. In females, invasion of the fallopian tubes or ovaries can result in salpingitis or ovaritis respectively, with any of these infections possibly resulting in sterility.

Chlamydia/Neisseria TaqMan PCR Kit,
100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Chlamydia/Neisseria TaqMan PCR Probe/Primer Set and Controls,
100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM42550 (100 preps)	Cat. TM42510 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
Chlamydia/Neisseria Primer & Probe Mix	280 μL	280 μL
Chlamydia/Neisseria Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

5 Layer Cell Factory Double Wide Mouth

Huayi Cell Factory is a robust and easy-to-use cell culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.

To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.

Specifications: 1 layer, 2 layers, 5 layers, 10 layers and 40 layers.

PRODUCT FEATURES

• The product is made of medical grade USP CLASS VI polymer polystyrene
• The product is made under a 100,00- class dust-free manufacturing site
• Surface TC treatment ensures efficient cell attachment
• The product assembled with ultrasonic welded technology
• Versatile port design facilitates both pouring and aseptic filling techniques
• Gamma radiation sterilization
• the cell culture surface area

of one 10-layer Cell Factory unit is equivalent to the area

of 36 T-175 flasks

Double Wide Mouth

Huayi Cell Factory is a robust and easy-to-use cell culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.

To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.

Specifications: 1 layer, 2 layers, 5 layers, 10 layers and 40 layers.

TGL18 Tabletop 21000rpm High Speed large capacity Swing out rotor 4x800ml universal Refrigerated Centrifuge Adapters for 5/10/15/50ml tubes. And holding Angle Rotors like 24×1.5/2ml, 6x50ml, 12x15ml…in high speed separation smooth.

TGL18 Universal Refrigerated Centrifuge Application:

Holding lots of Swing Out Rotors & Angle Rotors for options. Mainly used in animal and plant molecular biology, cell biology, food safety, life science, agriculture and forest science, animal husbandry science, biological products, pharmaceutical products and other fields of ideal equipment for the separation and precipitation of samples preparation.

TGL18 Universal Refrigerated Centrifuge Features:

1. With silent-block and shock absorbers that guarantees smooth and quiet operations & fast cooling.

2. The centrifuge body is made of high-quality steel, safe and reliable.

3.  Microprocessor digital control of all functions:speed, time,

temperature, acceleration/deceleration, RCF, 10 program memory, operation fault display.

4. Electronic safety lock alarm inside, just press down the top lid until it will lock in sealed and open up after separation automatically.

5. Easy open the centrifuge lid via pneumatic springs; the operation area of the centrifuge is at the front end.

6. RPM/RCF adjustable along with the speed setting calculating automatically.

7. 10 selective acceleration and deceleration rates for options

8. The self-diagnostic system provides a protection for imbalance over speed/voltage and electronic lock fault.

TGL18 Universal Refrigerated Centrifuge Technical Parameters:

Max Speed	21,000r/min	Max RCF	30,910xg
Max Volume	4x800ml	Power supply	AC220V,50HZ,7A
Timer	1~9h/59min	Noise	≤58dBA
Dimension	700×715×420mm	Net Weight	125KG
Speed Accuracy	±20r/min	Package(W*D*H)	Plywood box: 880x800x580mm

TGL18 Universal Refrigerated Centrifuge Matched Rotors:

Order No.	Rotor type	Max.Speed (rpm)	Max.Volume (ml)	Max.RCF (xg)
No30671	Swing out rotor	4,000	4x800ml	3,450
			Adapter for 4x23x5ml	3,450
			Adapter for4x19x10nl	3,450
			Adapter for4x14x15ml	3,450
			Adapter for4x5x50ml	3,450
No30801	Angle rotor	21,000	12×1.5/2ml	30,910
No30802	Angle rotor	15,000	40×0.5ml	22,920
No30803	Angle rotor	17,000	24×1.5/2ml	26,460
No30804	Angle rotor	13,500	30×1.5/2ml	19,340
No30844	Angle rotor	13,000	48×1.5/2ml	17,930
No30805	Angle rotor	16,000	16x5ml	22,020
No30806	Angle rotor	16,000	12x7ml	21,380
No30807	Angle rotor	10,000	12x15ml	11,840
No30808	Angle rotor	15,000	12x10ml	22,680
No30809	Angle rotor	15,000	8x20ml	22,680
No30811	Angle rotor	13,000	6x50ml	18,840
No30814	Angle rotor	12,000	6x100ml	16,390

The Corners of our Company____________________________________

Metal housing with 3-tiers protection. It can hold both swing out rotors and angle rotors in low speed and high speed spining to switch smoothly. And its Quiet noise, Less vibration & Fast cooling guarantee a perfect separation.