For scaling up and fully automating magnetic bead-based nucleic acid extraction
With the Flex Nucleic Acid Extraction Workstation, you can automate your NAE workflow at the scale you need to increase efficiency, reduce errors, and save hands-on time. Flex enables you to automate your nucleic acid isolation and purification workflows using any leading magnetic bead-based system on the market.
Optional add-ons can be purchased at a 10% discount when ordered with the Flex Nucleic Acid Extraction Workstation*
Detail
For scaling up and fully automating magnetic bead-based nucleic acid extraction
With the Flex Nucleic Acid Extraction Workstation, you can automate your NAE workflow at the scale you need to increase efficiency, reduce errors, and save hands-on time. Flex enables you to automate your nucleic acid isolation and purification workflows using any leading magnetic bead-based system on the market.
Optional add-ons can be purchased at a 10% discount when ordered with the Flex Nucleic Acid Extraction Workstation*
Other Products
D3125 HiPure DNA Micro Kit
Product Info
Document
Product Info
Introduction
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from small volume of blood, tissue and dry blood spots.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 1-10μl blood, <10mg tissue, urine, blood stain, seminal stain
Applications
PCR, southern bolt and virus detection, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Animal tissues, blood stain, urine, seminal stain and various forensic samples
Sample amount
Blood:1-100μl, Tissue:<10mg
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column
Principle
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA).
Advantages
Fast – several samples can be extracted in 20 minutes (after digestion)
High purity – purified DNA can be directly used in various downstream applications
High recovery – DNA can be recovered at the level of PG
Good repeatability – silica technology can obtain ideal results every time
Kit Contents
Contents
D312502
D312503
Purification Times
50 Preps
250 Preps
Buffer ATL
15 ml
60 ml
Buffer AL
15 ml
60 ml
Buffer GW1*
22 ml
66 ml
Buffer GW2*
20 ml
2 x 50 ml
Carrier RNA
310 μg
2 x 310 µg
Proteinase K
24 mg
120 mg
Protease Dissolve Buffer
1.8 ml
10 ml
Buffer AE
15 ml
60 ml
HiPure DNA Mini Columns I
50
2 x 125
2 ml Collection Tubes
100
5 x 100
Storage and Stability
Carrier RNA and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.
Experiment Data
Document
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from small volume of blood, tissue and dry blood spots.
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from insect tissue
Applications
PCR, southern bolt and virus detection, etc
Purification method
96 well DNA plate
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Insect tissue samples
Sample amount
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column
Principles
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High purity DNA – can be used in sensitive downstream applications such as multiplex and quantitative pcr
Good repeatability – suitable for extracting high-yield DNA from insect tissue samples
Safe – without phenol chloroform extraction and alcohol precipitation
Kit Contents
Contents
D313901
D313902
Purification Times
1 x 96
4 x 96
HiPure DNA Plate
1
4
2.2 ml Collection Plate
1
4
1.6 ml Collection Plate
1
4
0.5ml Collection Plate
1
4
Seal Film
8
32
Buffer ITL
30 ml
120 ml
Buffer IL
30 ml
125 ml
Buffer GW1
44 ml
2 x 110 ml
Buffer GW2
50 ml
3 x 50 ml
Proteinase K
50 mg
200 mg
Protease Dissolve Buffer
6 ml
15 ml
Buffer AE
20 ml
60 ml
Storage and Stability
Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.
Document
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
Propargyl-PEG4-acid is a PEG reagent with an alkyne and a terminal carboxylic acid. The carboxylic acid reacts with primary amine groups with the help of activators (e.g. EDC, or HATU). The 4-unit PEG spacer increases hydrophilicity. The alkyne can participate in copper-catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG4-acid is a PEG reagent with an alkyne and a terminal carboxylic acid. The carboxylic acid reacts with primary amine groups with the help of activators (e.g. EDC, or HATU). The 4-unit PEG spacer increases hydrophilicity. The alkyne can participate in copper-catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
