The Opentrons Flex 96-Channel Tip Rack Adapter allows the Opentrons Flex 96-channel pipette head to be compatible with Flex tips. The adapter is placed on the work table below the tip rack to ensure the rack is secured.
The Opentrons Flex 96-Channel Tip Rack Adapter allows the Opentrons Flex 96-channel pipette head to be compatible with Flex tips. The adapter is placed on the work table below the tip rack to ensure the rack is secured.
The Opentrons Flex 96-Channel Tip Rack Adapter allows the Opentrons Flex 96-channel pipette head to be compatible with Flex tips. The adapter is placed on the work table below the tip rack to ensure the rack is secured.
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from <10 mg insect tissue |
| Applications | PCR, southern bolt and virus detection, etc |
| Purification method | Midi spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Insect tissue samples |
| Sample amount | <10 mg |
| Elution volume | ≥15μl |
| Time per run | ≤30 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
Principles
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
| Contents | D312902 | D312903 |
| Purification Times | 50 | 250 |
| HiPure DNA Mini Columns I | 50 | 250 |
| 2ml Collection Tubes | 50 | 250 |
| Buffer ITL | 30 ml | 120 ml |
| Buffer IL* | 30 ml | 120 ml |
| Buffer GW1* | 22 ml | 110 ml |
| Buffer GW2* | 20 ml | 2 x 50 ml |
| Proteinase K | 24 mg | 120 mg |
| Protease Dissolve Buffer | 1.8 ml | 15 ml |
| Buffer AE | 15 ml | 60 ml |
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
PACE OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single reaction. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA. PACE OneStep RT-PCR Master Mix is highly efficient and sensitive, enabling detection of low-abundance RNA targets, particularly useful for applications such as gene expression analysis and viral RNA detection. PACE OneStep RT-PCR Master Mix demonstrates robust performance across a wide range of RNA templates and can be employed for both routine and challenging samples.
Genotype directly from RNA samples. RNA reverse transcription and cDNA PCR genotyping simultaneously in a single, one-step reaction.
These kits are designed to clean-up and concentrate RNA (including miRNA) from TRIzol® and TRI Reagent®, enzymatic reactions, in vitro transcription, labelling reactions, etc. These are robust kits for all clean-up and concentration purposes for up to 35 µg of RNA in solution. The purified RNA is of the highest purity and integrity, and can be used in a number of downstream applications. These kits purify all sizes of RNA, from large mRNA and ribosomal RNA down to miRNA, siRNA and lncRNA.
Norgen’s RNA Clean-Up and Concentration Kit provides a rapid method for the purification, cleanup and concentration of up to 35 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment and labeling. The kit elutes RNA in 20 µL. Complete 10 purifications in 20 minutes.
Norgen’s RNA Clean-Up and Concentration 96-Well Kit provides a rapid method for the purification, cleanup and concentration of up to 50 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment and labeling. The kit elutes RNA in 75 µL. Complete 10 purifications in 30 minutes.
Norgen’s RNA Clean-Up and Concentration Micro-Elution Kit provides a rapid method for the purification, cleanup and concentration of up to 10 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. This kit is made for eluting RNA in smaller volumes of 8 µL for all types of downstream applications, for the highest concentration of RNA sample. Complete 10 purifications in 20 minutes.
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| Kit Specifications (Spin Column) | |
| Maximum Column Binding Capacity | 35 μg |
| Size of RNA Purified | All sizes, including small RNA (<200 nt) |
| Maximum Amount of Starting Material | 35 μg of RNA |
| Minimum Elution Volume | 20 μL |
| Time to Complete 10 Purifications | 20 minutes |
| Average Recovery | ≥ 90% |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 23600 (50 preps) | Cat. 43200 (100 preps) | Cat. 25100 (192 preps) | Cat. 61000 (50 preps) |
|---|---|---|---|---|
| Buffer RL | 40 mL | 2 x 40 mL | 2 x 40 mL | 40 mL |
| Wash Solution A | 38 mL | 2 x 38 mL | 2 x 38 mL | 38 mL |
| Elution Solution A | 6 mL | 2 x 6 mL | 2 x 20 mL | 6 mL |
| Column Activation Solution | – | – | – | 30 mL |
| Micro Spin Columns | 50 | 100 | – | – |
| Micro-Elute RNA Spin Columns | – | – | – | 50 |
| 96-Well Plate | – | – | 2 | – |
| Adhesive Tape | – | – | 4 | – |
| Collection Tubes | 50 | 100 | – | 50 |
| 96-Well Collection Plate | – | – | 2 | – |
| Elution Tubes (1.7 mL) | 50 | 100 | – | 50 |
| 96-Well Elution Plate | – | – | 2 | – |
| Product Insert | 1 | 1 | 1 | 1 |