Opentrons Flex Tips, 50 µLOpentrons Flex 96-Channel Tip Rack Adapter (2 count)Opentrons Flex Tips, 50 µL

DBCO-C5-NHS ester is an amine-reactive compound, which can be used to modify an amine-containing molecule in organic media. This reagent isn’t soluble in aqueous media. The extended 5-carbon atom spacer arm improves solubility in commonly used organic solvents including dichloromethane, chloroform, THF, and ethyl acetate, and it also improves derivatization efficiency and stability of conjugates. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research use only.

DBCO-C5-NHS ester is an amine-reactive compound, which can be used to modify an amine-containing molecule in organic media. This reagent isn’t soluble in aqueous media. The extended 5-carbon atom spacer arm improves solubility in commonly used organic solvents including dichloromethane, chloroform, THF, and ethyl acetate, and it also improves derivatization efficiency and stability of conjugates. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research use only.

Description 

AccuBand™ 100 bp+3K DNA Ladder II is composed of 14 individual DNA fragments, presenting 3k, 2k, 1.5k, 1.2k, 1k, 900, 800, 700 600, 500, 400, 300, 200 and 100 bp sharp bands respectively. This product contains 2 enhanced bands (1k and 500 bp) for easy band identification. AccuBand™ 100 bp+3K DNA Ladder II is ready-to-use, containing loading buffer with dual color tracking dyes (orange G and Xylene cyanol FF). AccuBand™ 100 bp+3K DNA Ladder II provides a sufficient amount of DNA for clear observation of all DNA bands ranging from 100 bp to 3 kb, either in agarose gel or in polyacrylamide gel electrophoresis.

Features

• Sharp bands
• Suitable for polyacrylamide gel electrophoresis
• Quick reference— enhanced bands 
• Ready-to-use— premixed with loading dye for direct loading
• Stable— room temperature storage over 6 months

Source 

Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.

Range 

100 ~ 3,000 bp 

Concentration 

54.4 µg/ 500 µl 

Recommended loading volume 

5 µl/ well

Storage

Room temperature for 6 months
4°C for 12 months 
-20°C for 36 months 

Volcano3G® RT-PCR Probe 2x Master Mix contains all components required for general RT-qPCR
– optimized reaction mix for sensitive and reliable results
– engineered, truly thermostable Taq DNA polymerases with reverse transcriptase activity
– fast start function due to a hotstart aptamer formulation which prevents unspecific amplification at lower temperatures (<57°C)
– Pipetting aid in form of a blue dye for better visibility during aliquoting into well-plates
– included lysis function*

* = A sample extraction and sample lysis is not necessary as immediate hot PCR cycling conditions can brake cell- and virus membranes. Please see our published references to this product.

Note: Volcano3G® RT-PCR Probe 2x Master Mix is a unique master mix. If you are using it for the first time, we recommend to design primers to have higher melting/annealing temperatures (above 65°C). Additionally, for assay setup it is advised to perform RT-PCR test reactions with temperature gradients (i.e., for the annealing and extension steps) to identify the most proficient PCR protocol for your needs using our Volcano DNA polymerase products.

For research use and further manufacturing.

In case you are aiming to use our RUO products as components or for your development of e.g. an IVD medical device, please contact us.

Volcano3G® RT-PCR Probe 2x Master Mix contains all components required for general RT-qPCR
– optimized reaction mix for sensitive and reliable results
– engineered, truly thermostable Taq DNA polymerases with reverse transcriptase activity
– fast start function due to a hotstart aptamer formulation which prevents unspecific amplification at lower temperatures (