The Opentrons Flex Waste Chute GEN1 occupies position D3 and is used to accept waste such as tips, tip racks, and plates in high volumes. The gripper will pick up the consumable and dispose it into the chute based on your workflow needs. The waste chute is neccesary for all high-throughput workflows and can be paired with the Opentrons Flex Deck Expansion Set to maximize the throughput capabilities of the Opentrons Flex. The following consumables can disposed of into the waste chute:
– NEST 2 mL Deep Well Plate, V Bottom
– Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt
– NEST 96 well Plate Flat
– All Opentrons Flex tip racks
Detail
The Opentrons Flex Waste Chute GEN1 occupies position D3 and is used to accept waste such as tips, tip racks, and plates in high volumes. The gripper will pick up the consumable and dispose it into the chute based on your workflow needs. The waste chute is neccesary for all high-throughput workflows and can be paired with the Opentrons Flex Deck Expansion Set to maximize the throughput capabilities of the Opentrons Flex. The following consumables can disposed of into the waste chute:
– NEST 2 mL Deep Well Plate, V Bottom
– Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt
– NEST 96 well Plate Flat
– All Opentrons Flex tip racks
Other Products
Phosphate Assay Kit
Product Info
Document
Product Info
K-PHOS
SKU: 700004326
100 assays (manual) / 400 assays (auto-analyser)
Content:
100 assays (manual) / 400 assays (auto-analyser)
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Phosphate
Assay Format:
Spectrophotometer, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
360
Signal Response:
Increase
Linear Range:
0.1 to 10 μg of phosphate per assay
Limit of Detection:
0.16 mg/L
Reaction Time (min):
~ 20 min
Application examples:
Processed foods and drinks, bakery products, dairy products, waste water samples, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, etc.).
The Phosphate Assay Kit is a rapid, simple, reliable and accurate method for the specific measurement and analysis of phosphate in water, foodstuffs, beverages and other materials. The phosphate detection reaction employs the defined chemical substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG). In the presence of phosphate, the substrate is converted enzymatically by purine nucleoside phosphorylase (PNPase) to the products ribose 1-phosphate and 2-amino-6-mercapto-7-methylpurine. An application note describes the dephosphorylation reaction of phytic acid using phytase and alkaline phosphatase (sold separately), and subsequent phosphate detection using the reagents provided in K-PHOS.
This method is suitable for analysis using spectrophotometer and auto-analyser.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.
Need other products? See our complete list of assay kits.
Advantages
Chemically defined detection method
All reagents stable for > 2 years after preparation
Rapid reaction (~ 20 min)
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual and auto-analyser formats
Document
The Phosphate Assay Kit is a rapid, simple, reliable and accurate method for the specific measurement and analysis of phosphate in water, foodstuffs, beverages and other materials. The phosphate detection reaction employs the defined chemical substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG). In the presence of phosphate, the substrate is converted enzymatically by purine nucleoside phosphorylase (PNPase) to the products ribose 1-phosphate and 2-amino-6-mercapto-7-methylpurine. An application note describes the dephosphorylation reaction of phytic acid using phytase and alkaline phosphatase (sold separately), and subsequent phosphate detection using the reagents provided in K-PHOS.
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, urine, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
Details
Specifications
Features
Specifications
Main Functions
Extract viral RNA/DNA from non-cell/low cell content biological samples
Applications
RT-PCR，PCR，NGS
Products
Viral total nucleic acid
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Cell-free body fluid such as plasma, serum, soaking solution and tissue homogenate supernatant
Sample amount
200μl
Yield
2-10μg
Elution volume
≥30μl
Time per run
≤30 minutes
Liquid carrying volume per column
800μl
Binding yield of column
100μg
Principle
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA / RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA was finally eluted with low-salt buffer (10 Mm Tris, pH 8.0).
Advantages
Fast – several samples can be extracted in 20 minutes by column method
High quality – high purity total RNA can be directly used in various sensitive downstream applications
Safe – no phenol chloroform extraction required
Sensitive – DNA/RNA can be recovered at the level of PG
High yield – carrier RNA contained in the product maximize the recovery of trace nucleic acid
Kit Contents
Contents
IVD4173
Purification Times
100 Preps
HiPure Viral Mini Column
100
2ml Collection Tubes
200
PK/Carrier RNA
50 mg
Protease Dissolve Buffer
5 ml
Buffer AL
30 ml
Buffer MW1*
44 ml
Buffer MW2*
50 ml
RNase Free Water
15 ml
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.
Experiment Data
Document
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, urine, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
