Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt (25 count)
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Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt, RNase/DNase-free. Strong polycarbonate frame with thin-walled polypropylene wells for high-performance thermal cycling without warping. Ideal for use within automated liquid handlers with or without a gripper. 0.2 mL total well volume, 0.1 mL working volume. 25 count. Not for therapeutic use SP-2396.
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Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt, RNase/DNase-free. Strong polycarbonate frame with thin-walled polypropylene wells for high-performance thermal cycling without warping. Ideal for use within automated liquid handlers with or without a gripper. 0.2 mL total well volume, 0.1 mL working volume. 25 count. Not for therapeutic use SP-2396.
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NGS Normalization 96-Well Kit
Product Info
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Product Info
Overview
Removes primer dimers
Simultaneously clean-up and normalize PCR products
Fast (less than 20 minutes), high-throughput and easy processing using centrifugation
Sufficient elution volume (100 µL) for repeat or future assays
Non-magnetic bead purification
Norgen’s NGS Normalization 96-Well Kit allows for high-throughput NGS library PCR amplicon clean-up and normalization of NGS library PCR product concentration. The clean-up removes all PCR by-products including primers, dimers, enzymes and unincorporated nucleotides during the library prep PCR step. Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. The amount of Norgen’s matrix in each 96 well is optimized to have a limited binding capacity, thus each well can elute an equal concentration of PCR product. The process involves first adding 3 volumes of Buffer SK to the PCR product, and the mixture is then loaded onto the 96-Well Normalization Plate. The bound PCR amplicon is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified PCR amplicon is eluted with Elution Buffer B. The purified and normalized library PCR amplicon can then be used in NGS workflows and other sequencing applications.
Storage Conditions All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
DBCO-PEG8-amine TFA salt is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The hydrophilic PEG spacer increases the water solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
DBCO-PEG8-amine TFA salt is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The hydrophilic PEG spacer increases the water solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
Input volumes up to 200 µL
High yield and high quality DNA ready for any application
Fast and convenient spin column protocol
Norgen’s Blood Genomic DNA Isolation Mini Kit Dx is designed for the rapid preparation of genomic DNA from up to 200 µL of whole blood for subsequent in vitro diagnostic use. Both fresh and frozen anticoagulated blood may be used with this procedure. Purification is based on spin column chromatography as the separation matrix. Norgen’s column binds DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions.
This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of DNA followed by signal detection or amplification. Any diagnostic results generated using the DNA isolated with Norgen’s Blood Genomic DNA Isolation Mini Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.
To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used.
Norgen’s Blood Genomic DNA Isolation Mini Kit Dx is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques including experience with whole blood samples and DNA isolation.
Norgen’s Blood Genomic DNA Isolation Mini Kit Dx does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.
*Yield will vary depending on the type of blood processed
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers. The kit contains a ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.