Organophsphates are a class of pesticides that mechanistically target the acetylcholinesterase enzyme. Regulatory guidelines have been set to ensure our food and water are within the acceptable regulatory authority guidelines. Because most OPs are provided in their precursor form, organothiophosphate (i.e., Malathion, Diazinon, Chlorpyrifos, Azinphos, Dimethoate, Terbufos, Phosmet) Attogene’s organophosphate ELISA kit has been designed to detect organothiophosphates which are the main form of the compounds when applied in the field.
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HiDi® DNA Polymerase
Product Info
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Product Info
HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. This myPOLS Biotec enzyme family is optimized for this feature and is the first choice for applications that rely on this property such as allele-specific PCR (asPCR) that is also termed allele-specific amplification (ASA).This polymerase is also available as a full-length Taq DNA polymerase with a nuclease domain, featuring 100% compatibility with hydrolysis probes (TaqMan® probes etc.).Benchmarking with products of competitors conducted by us and others show that the HiDi® DNA polymerase family is the first choice for highly selective PCRs, such as genotyping by allele-specific PCR, HLA genotyping, analysis of single CpG methylation sites or the detection of mutations in a high background of wild-type sequences. By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of >10.000 wild-type copies straight away without any other tedious assay optimization.Several independently conducted studies show that HiDi® DNA polymerase is ideally suited for use in asPCR in numerous research areas ranging from mutation detection to genome editing. (read more) For research use and further manufacturing.In case you are aiming to use our RUO products as components or for your development of e.g. an IVD medical device, please contact us.
Casestudies: HiDi® DNA Polymerase: Applications from mutation detection to genome editing (read more)
Example Primer Design
Matching vs. mismatching nucleotide is placed at the 3′-end of the primer for best discrimination results.
Example Results – There´s no accounting for taste
Cilantro: some people love it in their food, some hate it. Here we are detecting a genomic SNP (rs72921001) in HeLa genomic DNA. This SNP is reported to be close to a number of genes coding for olfactory receptors. (Reference: Eriksson N. et al. (2012), “A genetic variant near olfactory receptor genes influences cilantro preference.”)
Considering, that only the C-allele specific primer is extended and yielding in a specific amplicon, we can conclude a genetic predisposition in disliking cilantro, as this SNP is significantly associated with detecting a soapy taste to cilantro.
Allele-specific PCRs were performed from 1 ng/µl of HeLa gDNA in the presence of a realtime dye, indicating the amplification of the C-allele specific primer only. The A-allele specific primer is discriminated, thus not amplified up to 50 cycles.
PCR products were subsequently analysed on a 2.5% agarose gel. Specific product is visualized by ethidium bromide staining at the amplicon length of 109 bp.
Document
HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. This myPOLS Biotec enzyme family is optimized for this feature and is the first choice for applications that rely on this property such as allele-specific PCR (asPCR) that is also termed allele-specific amplification (ASA).
Permagen’s 24 x 1.5 mL Microfuge Tube Magnetic rack is designed for magnetic bead separations from up to 24 tubes
Accommodates many common 1.5 mL Microcentrifuge and some 2.0 mL tubes
Tubes are angled and beads will be pulled to back wall allowing easy aspiration and tip tracking down the front wall of the tubes without disturbing bead pellet
Our 384-well PCR plates have a rigid fully-skirted frame which remains stable during thermal cycling.
About
Our 384-well plate has a rigid, fully-skirted frame which remains stable during thermal cycling. The rigidity of the plate lends itself to automated and high-throughput PCR environments where robotic handling maybe in use.
The 2-component design reduces the risk of evaporation due to a poor sealing, commonly seen with 1-component PCR plates. 100% virgin medical grade polypropylene is used for the 384 wells, with a maximum capacity of 45 µL Certified free from DNase, RNase, nucleases and human gDNA.
