Isolate DNA from a wide range of food materials. (e.g boiled, fluid, processed or raw food products)
No hazardous chemicals required (e.g. phenol or chloroform)
Effective lysis with Proteinase K and optional lysozyme treatment
Fast (less than 15 minutes hands-on time) and convenient processing using a rapid spin-column format
Wide compatibility with a variety of food products for GMO-DNA isolation
Universal protocol for food related pathogen DNA isolation (Gram positive and Gram negative)
This kit provides a rapid spin column method for the isolation and purification of total DNA from a wide range of food samples originating from animals or plants. The kit is designed for identification of GMO-DNA or animal components in food and feed and can be used for a wide range of starting materials including raw or processed food, meat, liquids, sauces and dairy products including milk, cheese and yogurt.
This kit also provides a convenient method for the detection of food-related pathogens and will isolate such DNA (enriched or as is) including Gram-positive bacteria, Gram-negative bacteria, yeast and fungi which may contaminate food sources. A number of pathogens have been tested including E. coli O157:H7, Staphylococcus, Listeria monocytogenes, Salmonella enterica & Campylobacter jejuni. The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR based detection, sequencing and genotyping.
Maximum Amount of Starting Material: Solid food material Liquid sample (e.g. milk or concentrated juice)
200 mg 1 mL to 1.5 mL
Time to Complete 10 Purifications
45 minutes
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
Samples Tested by PCR
Food Materials
Samples that Have Been Tested by PCR
Plant related
Cereal, Jam, Chocolate, Spices, Sauce
Animal related
Raw and processed meat products (e.g. ham, beef jerky, taco seasoned ground beef, pork and sausage)
Dairy product
Milk, Yogurt, Cheese
Pathogens (enriched from food samples)
E. coli O157:H7 from food sample Staphylococcus from milk Listeria monocytogenes from milk Salmonella enterica from raw meat Campylobacter jejuni from milk
Convenient – With the ready-to-use Master Mix, the user needs only to add template to the master mix and enzyme in order to set up the reverse transcriptase reaction
Time Savings – Set up RT reactions in a shorter time since less pipetting steps are required
Cost Efficient – No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix kits
High Sensitivity and Yield – the optimized Master Mix allows for highly sensitive amplifications with high yields of PCR products
Robust Enzyme – broad range of working temperatures from 37°C to 60°C. Capable of amplifying difficult templates with a high degree of reproducibility
TruScript Reverse Transcriptase is Available in Three Convenient Formats:
This kit contains 5X RT Buffer and a vial of TruScript Enzyme Mix (200 units/µL). This enzyme can be used for reverse-transcription reactions with any user-supplied primers.
2. TruScript First Strand cDNA Synthesis Kit (Cat.#54420)
This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of total RNA (both poly A- or non-poly A-containing transcripts).
The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and primers (oligo dT and random hexamers) for effective cDNA synthesis from total RNA transcripts.
3. TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.#54400)
This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of messenger RNA (poly A-containing transcripts).
The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and oligo dT primer for the effective cDNA synthesis from total RNA transcripts or enriched mRNA sample.
Which TruScript Kit is Best for Your Needs?
TruScript Reverse Transcriptase
TruScript First Strand cDNA Synthesis Kit for mRNA
TruScript First Strand cDNA Synthesis Kit
Kit contains only reverse transcriptase enzyme and buffer (no primers or other buffer components)
Your template is enriched mRNA
Your template is total RNA (poly A OR non-poly A-containing transcripts)
Kit contains oligo dT
Kit contains both oligo-dT primer and Random Hexamers
Kit contains reaction buffer with nucleotides and primer
with oligo-dT
with oligo-dT and random hexamers
You have your own first strand synthesis primer
Your template is microRNA (using your own primers)
Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C. These reagents should remain stable for at least 1 year in their unopened containers.
