The Opentrons® OT-2 is a high-precision, open-source liquid handler designed to be easy for scientists to use and flexible enough to automate many application workflows. This robot and its accompanying automation platform are used to automate hundreds of protocols and workflows in genomics, proteomics, cell-based assays, and drug discovery.
The OT-2 has a user-friendly, plug and play graphical interface to design protocols compatible with any ANSI/SBS compliant or automation friendly labware. The base model includes an Opentrons GEN2 Single-Channel Pipette; you can also use up to two pipettes at the same time. Additional pipettes can be purchased separately and are easily interchangeable by the user. Consumables and other labware are sold separately.
Detail
The Opentrons® OT-2 is a high-precision, open-source liquid handler designed to be easy for scientists to use and flexible enough to automate many application workflows. This robot and its accompanying automation platform are used to automate hundreds of protocols and workflows in genomics, proteomics, cell-based assays, and drug discovery.
The OT-2 has a user-friendly, plug and play graphical interface to design protocols compatible with any ANSI/SBS compliant or automation friendly labware. The base model includes an Opentrons GEN2 Single-Channel Pipette; you can also use up to two pipettes at the same time. Additional pipettes can be purchased separately and are easily interchangeable by the user. Consumables and other labware are sold separately.
The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity and signal intensity as well as low background and better compatibility with cDNA templates derived directly from reverse transcription reaction mixture. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Q-PCR Master Mix is also compatible with a ROX reference dye if recommended by the manufacturer of the qPCR system.
Features
High sensitivity and signal intensity
Smart blue contrast dye as a visual aid for reaction setup
Better compatibility for reverse transcription
Low background
Storage
Protected from light. Aliquot to avoid multiple freeze-thaw cycles. -20°C for 12 months
Document
The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity and signal intensity as well as low background and better compatibility with cDNA templates derived directly from reverse transcription reaction mixture. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Q-PCR Master Mix is also compatible with a ROX reference dye if recommended by the manufacturer of the qPCR system.
Sequentially purify total RNA (and miRNA), DNA and proteins from a single sample
No sample splitting or need to use phenol or precipitation methods
Purify RNA/DNA/Protein from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
RNA/DNA/Protein Purification Plus Kit
This kit provides a rapid method for the high throughput isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, or yeast. The kit employs two columns: 1) for gDNA purification and 2) for RNA purification utilizing Norgen’s resin columns (superior for the binding of all RNA sizes including miRNA). The proteins are also purified on the second column after RNA elution. The proteins are eluted in buffer and are ready for downstream application without any further clean up required. The proteins can be quantified directly, used in western blots, ELISA or mass spectrometry. This kit provides a rapid spin-column method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants.
RNA/DNA/Protein Purification Plus Micro Kit
This kit provides a rapid spin-column method for the isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, microdissected samples including LCM, stem cells, sorted cells, and CTC. The total RNA, genomic DNA and proteins are all column purified in less than 30 minutes. The RNA and DNA can be eluted in as little as 20 µL while the protein can be eluted in as little as 50 µL. This kit provides the same performance as if the samples were isolated from dedicated kits.
RNA/DNA/Protein Purification 96-Well Plus Kit
The kit employs two plates: 1) for DNA purification and 2) for RNA purification utilizing Norgen’s resin (superior for the binding of all RNA sizes including miRNA). Please see the protocol schematic below.
Simultaneously clean-up and normalize PCR products
Fast (less than 20 minutes), high-throughput and easy processing using centrifugation
Sufficient elution volume (100 µL) for repeat or future assays
Non-magnetic bead purification
Norgen’s NGS Normalization 96-Well Kit allows for high-throughput NGS library PCR amplicon clean-up and normalization of NGS library PCR product concentration. The clean-up removes all PCR by-products including primers, dimers, enzymes and unincorporated nucleotides during the library prep PCR step. Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. The amount of Norgen’s matrix in each 96 well is optimized to have a limited binding capacity, thus each well can elute an equal concentration of PCR product. The process involves first adding 3 volumes of Buffer SK to the PCR product, and the mixture is then loaded onto the 96-Well Normalization Plate. The bound PCR amplicon is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified PCR amplicon is eluted with Elution Buffer B. The purified and normalized library PCR amplicon can then be used in NGS workflows and other sequencing applications.
Storage Conditions All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
