
Opentrons OT-2 Single-Channel Electronic Pipettes automate liquid handling and pipetting for most daily wetlab applications. Best for low to medium throughput workflows with high precision.
Opentrons OT-2 Single-Channel Electronic Pipettes automate liquid handling and pipetting for most daily wetlab applications. Best for low to medium throughput workflows with high precision.
Opentrons OT-2 Single-Channel Electronic Pipettes automate liquid handling and pipetting for most daily wetlab applications. Best for low to medium throughput workflows with high precision.
Blood samples contain rich DNA, including mitochondrial DNA, genomic DNA, circulating DNA (mostly released into blood after tumor cell apoptosis) in white blood cells, as well as parasitic viral or microbial DNA. These DNA are important parameters in clinical testing or diagnosis, which are also valuable materials for medical research. There are three main issues with extracting DNA from blood samples:
1. The sample is highly infectious, posing great harm to operators and the environment.
2. The source of DNA is complex and aportion of the nucleic acid, such as viral DNA or free DNA, may be lost during the operation, leading to downstream detection failure;
3. Blood sample contains a large amount of impurities and inhibitory factors.
Currently there are many methods available for extracting DNA from whole blood samples, such as phenol chloroform extraction, salting out method, etc. However, these methods require pre-treatment of blood sample, which removes red blood cells and isolate white blood cells in the first step. Due to the requirement that it cannot inactivate or kill pathogens during the process of removing red blood cells, the waste liquid (red blood cell lysate) and consumables may be contaminated by pathogens and become infectious, posing a danger to the entire laboratory environment and operators. In addition, during the process of removing red blood cells, useful nucleic acid information such as viruses, microorganisms, or circulating DNA is also lost, leading to experiment or detection failures.
The HiPure Blood DNA Kits series provided by Magen Company uses silica gel column purification technology, which can directly lyse whole blood samples without the need for white blood cell separation. Whole blood samples are directly mixed with lysates and proteases, resulting in the inactivation of pathogens, greatly reducing the infectivity, environmental pollution, and the chance of operators being infected. Due to the direct lysis and digestion of samples, except lymphocyte DNA, other circulating DNA as well as DNA from viruses and microorganisms, can also be recovered.
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue, whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from blood, tissue, culture cells, swab, blood spots using 96 plate |
Applications | PCR, southern bolt and virus detection, etc |
Purification method | 96 well plate |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Blood, serum, plasma, milk, saliva, and other liquid samples and cultured cells |
Sample amount | |
Elution volume | |
Time per run | |
Liquid carrying volume per column | |
Binding yield of column |
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Contents | D311701 | D311702 |
Purification Times | 1 x 96 | 4 x 96 |
HiPure gDNA Plate | 1 | 4 |
96 well Plate (2.2ml) | 1 | 4 |
1.6ml Collection Plate | 1 | 4 |
0.5ml Collection Plate | 1 | 4 |
Silicon Seal Tape | 1 | 4 |
Seal Film | 5 | 25 |
Buffer ATL | 30 ml | 100 ml |
Buffer AL | 30 ml | 100 ml |
Buffer DW1 | 60 ml | 250 ml |
Buffer GW2 | 50 ml | 2 x 100 ml |
Proteinase K | 50 ml | 200 ml |
Protease Dissolve Buffer | 5 ml | 15 ml |
Buffer AE | 30 ml | 120 ml |
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Name | CAT NO | Sample amount | Leukocyte protocol* | Colum type | Elutio volume | Average yield | Time per run |
HiPure Blood DNA Mini Kit | D3111 | 10-200μl | 1ml | 2ml column | ≥20μl | 5-9μg/200μl | ≤30 minutes |
HiPure Tissue&Blood DNA Midi Kit | D3113 | 0.2-2ml | 10ml | 1.5ml column | ≥300μl | 20-40μg/1m | ≤80 minutes |
HiPure Tissue&Blood DNA Maxi Kit | D3115 | 3 -10ml | 10ml | 15ml column | ≥700μl | 20-40μg/1m | ≤90 minutes |
HiPure Tissue&Blood DNA 96 Kit | D3117 | 1-200μl | 1ml | 96 well plate | 3-8μg/200μl |
Blood samples contain rich DNA, including mitochondrial DNA, genomic DNA, circulating DNA (mostly released into blood after tumor cell apoptosis) in white blood cells, as well as parasitic viral or microbial DNA. These DNA are important parameters in clinical testing or diagnosis, which are also valuable materials for medical research. There are three main issues with extracting DNA from blood samples:
029172 Oxidase Test Reagent
Usage: For Oxidase Test.
Specification:10pcs/box
10pcs/box
K-CITR
SKU: 700004274
72 assays (manual) / 720 assays (microplate) / 840 assays (auto-analyser)
Content: | 72 assays (manual) / 720 assays (microplate) / 840 assays (auto-analyser) |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 2 years under recommended storage conditions |
Analyte: | Citric Acid |
Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
Detection Method: | Absorbance |
Wavelength (nm): | 340 |
Signal Response: | Decrease |
Linear Range: | 1.0 to 100 µg of citric acid per assay |
Limit of Detection: | 0.491 mg/L |
Reaction Time (min): | ~ 5 min |
Application examples: | Grape juice, wine, beer, fruit juices, soft drinks, tea, dairy products (e.g. cheese), meat, processed meat, vegetable and fruit products, bakery products, paper, pharmaceuticals, cosmetics and other materials (e.g. biological cultures, samples, etc.). |
Method recognition: | Methods based on this principle have been accepted by MEBAK, OIV, EU, ISO2963, AOAC and IFU22 (NOTE: If the enzyme oxaloacetate decarboxylase is present in the sample, some of the oxaloacetate product is converted to pyruvate. Therefore, to ensure citric acid is measured quantitatively, D-lactate dehydrogenase (D-LDH) is employed to efficiently convert any pyruvate produced into D-lactate and NAD+). |
The Citric Acid test kit is a flexible and simple method for the rapid and reliable measurement and analysis of citric acid (citrate) in foods, beverages and other materials.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
View our full list of organic acid test kits.
Advantages
The Citric Acid test kit is a flexible and simple method for the rapid and reliable measurement and analysis of citric acid (citrate) in foods, beverages and other materials.
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