OT-2 Tips, 1000µL

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Eight discrete fragments from 50 bp to 2000 bp
• Higher intensity reference band at 500 bp

The FastRunner DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains eight discrete fragments ranging from 50 bp to 2000 bp with a higher intensity reference band at 500 bp. This Ladder is ideal for quick sizing of PCR products and restriction digests.

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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FastRunner DNA Ladder (Cat# 12800) – 100 loads

Ladder Properties:
• Eight discrete bands, ranging from 50 bp to 2000 bp
• Higher intensity band at 500 bp for easy reference

Fragment	Size (bp)	Mass (ng)
1	2000	104
2	1500	88
3	1000	68
4	750	59
5	500	93
6	300	28
7	150	35
8	50	25

Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 
Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Description

This fast screening test kit contains 10 tests with everything needed for accurate results of unsafe lead levels in the field. 

This is a revolutionary product designed to make Lead testing in water safe, easy, and affordable.  This fast screening test kit contains 10 tests with everything needed for accurate results of unsafe lead levels. With reliable results in only 10 minutes, this test kit clearly gives results confirming Lead in water and conforming to the EPA guideline of 15 ppb (µg/L). The kit can test water quality from various sources including faucets, wells (ground water), and home purification systems.

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Standards and Regulations for Lead [ACCLPP 2012]

• EPA: Drinking Water: 15ppb
• FDA: Juice: 50ppb
• EPA: Residential Soil: 400 ppm (play areas), 1200 ppm (non-play areas)
• CPSC: Paint: 90ppm
• FDA: Bottled drinking water: 5ppb

High Blood lead levels (i.e., greater than 700ppb) can cause serious health effects, including seizure, coma, and death.  Blood levels as low as 100ppb have been associated with adverse effects on cognitive development, growth, and behavior among children aged 1-5 years.

Sample method:

1. Take a first-draw sample

Immediately after opening a faucet or valve, collect a 250 mL sample. This sample should be from each tap used for consumption.

2. Take a flush sample

If first-draw sample results show elevated lead levels of 5 ppb or higher, collect a flush sample. To do this, ensure water has not been used for between 8 to 18 hours, then collect the sample at 30 seconds.

3. Take sequential samples

If you want to test a lead service line, collect 8 to 10 sequential samples, depending on how far the line is from the tap.

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• CDC – Centers for Disease Control and Prevention
  CPSC – Consumer Products Safety Commission
  EPA – Environmental Protection Agency
• FDA Takes Steps to Limit Lead in Juice to Further Reduce Exposure to Toxic Elements in Foods

Screening of Lead in water samples 5-10 ppb
Format: 10 tests (5 tests/5 controls)
Run Time: 15 Minutes