Overall solution for thermostatic detection of largemouth bass rhabdovirus
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This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39ºC~42ºC), with the help of auxiliary proteins and single-strand binding proteins,the recombinase and primers form a complex; Source search and combine the target homology domain, at this time, a D-loop region is formed at the homology position and strand exchange begins;along with the dissociation of the recombinase from the complex,the polymerase also binds to the 3′ end of the primer and begins chain extension.It is suitable for laboratory-level DNA amplification and DNA amplification for other detection purposes.
Detail
Product Details
Sensitivity:
High
Kit Type:
RNA
Format:
Lyophilized
Reaction Volume:
50 μL
Reagents:
Enzymes, Buffers, And Primers
Product Name:
Overall Solution For Thermostatic Detection Of Largemouth Bass Rhabdovirus
Isolate high quality DNA from a broad variety of phage strains
High yields of total DNA
Fast and easy processing using a rapid spin-column format
No phenol or chloroform extractions or cesium chloride banding required
High yields of DNA recovered3-15 µg DNA from 106-1010 pfu/ mL of enriched phages
This kit provides a rapid spin column method for the purification of total DNA from a broad spectrum of bacteriophages propagated in bacteria grown in liquid cultures. The DNA is isolated without the use of phenol, chloroform or cesium chloride banding procedures. The spin-column based procedure is rapid and can be completed in less than 45 minutes. The kit is highly efficient for processing small volumes of phage supernatant (500 µL – 1 mL) and with the optional DNase and Proteinase K treatments phage DNA yields are maximized while host DNA contamination is minimized. Purified total phage DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), sequencing, cloning, Southern Blot and more.
3-15 µg DNA from 106-1010 pfu/mL of enriched phages
Time to Complete 10 Purifications
45 minutes
* Average yields will vary depending upon a number conditions used and developmental stage.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Compact and stylish designed, the BioShake iQ high-end thermoshaker lets you perform all your standard mixing runs with a minimum of adjustments. The BioShake iQ offers outstanding performance to handle a wide range of applications across biotechnology, pharmaceutical and academic research.
Small. Light. Efficient. Quiet.
For microplates, tubes and glass vials
Fast & high-precision heating
Temperature range: ambient to 99°C
Precise mixing from 200 – 3,000 rpm
Wide range of thermo adapters
Stylish aluminium housing
ADVANCED TECHNOLOGY
Save time.
By combining the mixing operation with the incubation phase, reaction process times and operator workload are reduced and efficiency of many procedures is increased, resulting in a higher throughput.
The first-class finished aluminium housing gives the BioShake iQ its essential functionality. It provides a high amount of security, device stability and ensures a long service life.
GENTLE MIXING OF SAMPLES
By planar orbital motion
German designed and manufactured, BioShake iQ thermoshaker offer an ultra-efficient, 2-dimensional shaking axis so that samples mix completely in a fraction of the time of competing systems.
The mixing orbit of 2.0 mm is always constant. The orbital shaking is precisely controlled, in fact, that you need never spin down your plates after mixing. Even tubes, vials, high density plates, or low sample volumes, offer no obstacle for these precision tools.
Fully adjustable between 200 rpm and 3,000 rpm, well beyond the speeds of most other brands, guarantees fast, splatter-free, mixing for tubes, glass vials or across an entire 384-well microplate.
HOMOGENEOUS HEATING
Using large-area heating source in combination with digital sensor electronic
The BioShake iQ heats from ambient to 99°C in a short time, provides ease of use features, amazing comfort and maximum safety.
Within the BioShake iQ thinnest large-area heating elements and sensors are integrated. During the heat up time the special electronic control system guarantees the same temperature for all vessels with minimal deviations. Temperatures are selectable from ambient temperature to 99°C in steps of 1.0°C. The nominal regulation accuracy is better than ±0.1°C with a uniformity of temperature distribution better than ±0.5°C at 45°C across the heating surface.
Exchangeable thermo adapter plates and thermal blocks enable a perfect adaptation to microplates, tubes or glass vials.
UNIVERSAL SPRING CLAMPS
Suitable for all microplates
In the corners eight spring clamps are inserted for easy fixation of skirted microplates anddeep well plates in SBS format 127.7 x 85.5 mm without an attached adapter. These spring clamps lock the microplate quickly and safely, even at the highest mixing frequencies.
For flexible and deformable PCR plates we recommend using of the related PCR adapter.
Fast mixing processes with frequent plate changes can be performed in the routine as easy and safe.
COMFORT
Simple intuitive operation and programming
The BioShake iQ is operated from the front face of the enclosure which includes touch buttons and a display.
Special buttons for start, stop and storage of time and mixing modes enable the instrument to run complex applications. This opens new points of view on the daily laboratory work and optimizes routine application enormously.
The short mix button allows short and fast mixing in between.
The two line LCD display guarantees simultaneous and safe reading of all programmed and measured parameters as time, mixing frequency and temperature.
EXCHANGABLE THERMO ADAPTERS
For a wide range of applications
The BioShake iQ comes with a variety of standardized and specific adapter plates. The replacement of the adapters are very simple.
Perfect shaped adapters allow an optimal fit for standard tubes, lysis tubes, microplates, glass vials and other sample vessels. An excellent temperature uniformity and homogeneity is guaranteed for all samples.
UNIQUE DESIGN MEETS HIGH-END TECHNOLOGY
First-class finished aluminium housing
Unique and efficient design combined with the most compact housing result in beautiful and well-defined laboratory equipment. Therefore, the first-class finished aluminium housing gives the BioShake iQ its essential functionality. It provides a high amount of security, device stability and ensures a long service life.
Safety of the user and ease of use are clearly a priority.
MADE IN GERMANY
High-end instruments for daily routine
A perfectly harmonious blend of high-tech and handmade. “Made in Germany” has always been a recipe for success for QInstruments. 100% of QInstruments production takes place in Germany.
The focus is on human diligence and on being environmentally friendly. The company upholds its quality guarantee by means of consistent quality management. For more than 15 years these lab automation professionals from Jena have used only high-quality materials to ensure sustainable production, applied innovative thinking and undertaken research in a future-oriented way – all as a matter of course.
Document
For microplates, tubes and glass vials Fast & high-precision heating Temperature range: ambient to 99°C Precise mixing from 200 – 3,000 rpm Wide range of thermo adapters Stylish aluminium housing
1. The instantaneous centrifugal function rises to the maximum speed for about 6 seconds, allowing for rapid detachment of hanging droplets from the wall;
2. Faster acceleration and deceleration rate, required to complete the experiment in a shorter time;
3. Door cover protection, overspeed and imbalance detection system, capable of real-time monitoring of the centrifugal process, ensuring the safe operation of the instrument; When the operation ends, an error occurs, or an imbalance occurs, the sound signal prompts, and the operation stops at the same time. The LCD displays the result code
Host Parameters
Product model
Mini-3
Input power supply
DC24V/2.75A
Input power
55W
Motor/Drive Method
DC24V brushless DC variable frequency motor
Display method
High brightness LCD screen
Effective centrifugal time
1-99min.1-59sec
speed
300~3000±15rpm
Speed step increase
10rpm
Maximum capacity
2 *96 well PCR plates/ELISA plates
MAX. RCF
608 xg
Misoperation/alarm failure
Sound prompt+display code
weight
3.9Kg
Noise
60dB (A)
Fastest acceleration time
<6s
Fastest deceleration time
<5s
Permissible ambient temperature/relative temperature
+5-40°C/80%
Document
The micro porous plate centrifuge adopts international advanced design concepts and manufacturing technology, with a smooth and beautiful appearance, compact and stable structure, adhering to the advantages of high universality and easy operation. It also has the functional characteristics of “smooth start” and “smooth braking”; This micro porous plate centrifuge is very suitable for 96 or 384 well and small capacity micro porous plates, as well as various standard PCR micro porous plates with or without skirts .
