20 bp DNA Ladder on 3.6% of agarose gel, 8% and 12% of acrylamide gel.

• For sizing and quantification of double strand DNA fragments.
• Composed of eight bands as shown on right.
• Premixed with 6X DNA loading buffer for direct gel loading.

• For sizing and quantification of double strand DNA fragments.
• Composed of eight bands as shown on right.
• Premixed with 6X DNA loading buffer for direct gel loading.

Description

Description

Attogene’ s RNase Inhibitor is a protein-based ribonuclease inhibitor which noncovalently binds and inactivates a wide variety of RNases in a range of temperature (37–65°C) and pH (5.5–8.5) conditions. This product is distinct from placental RNase Inhibitor protein in that it inactivates RNases I and T1 in addition to RNases A, B and C. NA2021 is distinct from RNase Inhibitor protein in that it is less expensive, have more robust interactions with RNAses.

MADE AND FUNCTIONALLY TESTED AT ATTOGENE (MADE IN AUSTIN TEXAS – USA)

RNase Inhibitor (10,000 units)
Concentration: 40 units/μL
Volume: 0.25 mL
Storage Conditions: Store at 2°C – 8°C
Inhibits: RNases A, B, and C
Supplied in: 1X PBS with 0.05% Sodium Azide.

Introduction

This product is suitable for rapid extraction of DNA from FFPE sample. This kit uses two combination methods. High-salt Bind is conducive to remove pigments or polysaccharides from complex FFPE samples, so as to improve the purity of nucleic acid and avoid blocking aligent 2100. Alcohol mediated adsorption is conducive to improve the nucleic acid yield of high-yield samples.

Details

Specifications

Features	Specifications
Main Functions	Isolation high pure total DNA from FFPE using high bind beads
Applications	PCR and viral DNA detection, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Paraffin embedded tissue samples
Sample amount	1-6 slices of 10-20μm
Elution volume	≥30μl
Time per run	≤60 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

• High yield – most optimal process, recovery up to 90%
• Economy – less than 50% of the price of Qiagen and other imported products
• High purity – OD 260/280 : 1.7-1.9, OD 260/230 : 1.5-2.0

Kit Contents

Contents	D632301D	D632302D
Purification Times	48 Preps	96 Preps
MagPure Particles N	1.1 ml	2.5 ml
RNase A	10 mg	20 mg
Proteinase K	24 mg	48 mg
Protease Dissolve Buffer	3 ml	6 ml
Buffer DPS	60 ml	100 ml
Buffer ATL	15 ml	30 ml
Buffer BST1	30 ml	60 ml
Buffer BW1	13 ml	44 ml
Elution Buffer	15 ml	30 ml

Storage and Stability

Proteinase K, RNase A and MagPure Particles N should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

This product is suitable for rapid extraction of DNA from FFPE sample. This kit uses two combination methods. High-salt Bind is conducive to remove pigments or polysaccharides from complex FFPE samples, so as to improve the purity of nucleic acid and avoid blocking aligent 2100. Alcohol mediated adsorption is conducive to improve the nucleic acid yield of high-yield samples.