PACE OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single reaction. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA. PACE OneStep RT-PCR Master Mix is highly efficient and sensitive, enabling detection of low-abundance RNA targets, particularly useful for applications such as gene expression analysis and viral RNA detection. PACE OneStep RT-PCR Master Mix demonstrates robust performance across a wide range of RNA templates and can be employed for both routine and challenging samples.
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Shrimp Alkaline Phosphatase
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ArcticZymes’ Shrimp Alkaline Phosphatase (SAP), including its recombinant version (rSAP), is the gold standard and the first heat-labile, all-purpose alkaline phosphatase on the market.
Originally isolated from Pandalus borealis (Arctic shrimp), it has been purified from a recombinant source since 2010. Unlike other alkaline phosphatases, rSAP can be fully inactivated by a short heat treatment of 15 minutes at 65°C.
This added convenience through complete heat inactivation has made SAP one of the most sold DNA modifying enzymes.
For added flexibility, especially when lyophilisation may be desired, rSAP is also available in a Glycerol FREE format. First launched in 1993, SAP’s unique features continue to make it a valuable tool in various applications.
Key Features
Gold standard heat labile, all-purpose alkaline phosphatase.
Completely inactivated after 5 min at 65°C or 1 min at 75°C.
Robust: Active in most restriction enzyme buffers, no need for supplemental zinc or other additives for activity.
Simple, hassle-free dephosphorylation of DNA, RNA, dNTPs and proteins for subsequent use in cloning or end-labelling of probes.
No vector purification necessary.
Easy inactivation of unincorporated dNTPs in PCR products prior to DNA sequencing or SNP analysis.
Ideal for MALDI-TOF, cloning, HLA typing, genotyping and sequencing.
Preparing substrate in protein kinase studies.
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ArcticZymes’ Shrimp Alkaline Phosphatase (SAP), including its recombinant version (rSAP), is the gold standard and the first heat-labile, all-purpose alkaline phosphatase on the market.
Formaldehyde is an organic compound with the formula CH2O. It is mainly used in the production of industrial resins but has been found to be used as a preservative, disinfectant and biocide. Because of its toxicity and volatility formaldehyde poses a significant risk to human health. Attogene test uses the property of formaldehyde to react with the Formaldehyde Reaction Powder (FRP) to form a purple-red tetrazine. The formaldehyde concentration is measured by visual comparison of the reaction with the color scale derived from the Color Card.
Measuring range / color- Number of scale graduation
Attogene test uses the property of formaldehyde to react with the Formaldehyde Reaction Powder (FRP) to form a purple-red tetrazine. The formaldehyde concentration is measured by visual comparison of the reaction with the color scale derived from the Color Card.
