PC Biotin-PEG3-alkyne is useful for introducing a biotin moiety to azide-containing biomolecules using Cu(I)-catalyzed Click Chemistry. PEG spacer provides better solubility to the labeled molecules in aqueous media. Captured biomolecules can be efficiently photoreleased using near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2). Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
PC Biotin-PEG3-alkyne is useful for introducing a biotin moiety to azide-containing biomolecules using Cu(I)-catalyzed Click Chemistry. PEG spacer provides better solubility to the labeled molecules in aqueous media. Captured biomolecules can be efficiently photoreleased using near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2). Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Fig 1. Only three steps will be need for Sample Preparation and only 20 minitutes will be taken for Sample Preparation.
Fig 2. Seven concentration samples of 0.3fg/μL, 3fg/μL, 30fg/μL, 300fg/μL, 3pg/μL, 30pg/μL, 300pg/μL were detected. CV of each concentration was < 30%, Regression coefficient associated with standard solutions was 0.99992, and amplification efficiency was 100.370%.
Fig 3. Five concentration samples of 0.1fg/μL, 0.3fg/μL, 0.5fg/μL, 1fg/μL and 3fg/μL were detected, and 10 multiple wells were detected for each concentration. The CV of concentration values of samples with 0.3fg/μL and above concentrations were less than 30%.
Fig 4. DNA recovery can be determined by including samples spiked with known DNA amounts which are prepared from the corresponding DNA standards. Typically, the range for this value varies from 70% to 130%.
Fig 5. Only one Reagent for qPCR MIX.
Note: Price not include shipment & duty, contact us to get full quote.
The resDNASEQ CHO Residual DNA Quantitation kit is designed for the quantification of residual DNA from CHO, in cell lines which are used for production of biopharmaceutical products. The Ducky Bio residual DNA CHO Assay, based on proven real-time qPCR technology, makes testing of residual DNA from the Chinese hamster ovary (CHO) cell line rapid, specifc. The PCR-based assay is sensitive and specific for DNA from the CHO cell line and not subject to detection of human or environmental DNA that might be introduced during sample handling. The kit was developed to meet the sensitivity requirements defined by WHO (10 ng CHO DNA per therapeutic dose).
Propargyl-PEG13-alcohol can reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to form stable triazole linkage. The hydrophilic PEG units increase the water-solubility of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG13-alcohol can reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to form stable triazole linkage. The hydrophilic PEG units increase the water-solubility of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
A rapid, high performance dye-terminator removal process based on the paramagnetic bead technology. The paramagnetic bead format requires no centrifugation or filtration and is easily performedmanually or fully automated for high throughput dye-terminator removal. Compared to similar systems, this product produces sequences with longer Phred 20 read lengths and higher signal intensities than any other purification technology.
Specifications
| Features | Specifications |
| Main Functions | Removal of free fluorescent dye from sequencingsolution (Replace Beckmen or agencourt CleanSeq) |
| Applications | Automated sequences |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | DNA doped with fluorescentdye |
| Sample amount | 5μl |
| Recovery | 90% |
| Elution volume | ≥25μl |
| Operation time | ≤50 minutes |
The CleanSeq method contains magnetic particles in an optimized binding buffer to selectively capture sequencing extension products. The protocol can be performed directly in the thermal cycling plate. Unincorporated dyes, nucleotides, salts and contaminants are removed using a simple washing procedure. The purification procedure is amenable to a variety of automation platforms since it requires no centrifugation or vacuum filtration.
Advantages
Kit Contents
| Contents | BCS-5 | BCS-50 | BCS-500 |
| CleanSeq Beads | 5 ml | 50 ml | 500 ml |
Storage and Stability
CleanSeq Beads should be stored at 2-8°C upon arrival and is stable up to 6 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. Mix CleanSeq Beads well before using. The reagent should appear homogenous and consistent in color.
DO NOT FREEZE.
A rapid, high performance dye-terminator removal process based on the paramagnetic bead technology. The paramagnetic bead format requires no centrifugation or filtration and is easily performedmanually or fully automated for high throughput dye-terminator removal. Compared to similar systems, this product produces sequences with longer Phred 20 read lengths and higher signal intensities than any other purification technology.
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
Copyright © 2024 A3P Scientific Co., Ltd. All rights reserved. Web by Mountain Studio
Privacy Policy | Terms of Use | Site Map