PCR Plate Adapter for Heater-Shaker Module

Overview

• Purification and enrichment of intact cell culture media exosomes for functional studies
• Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
• Versatile cell culture media input volumes
• No phenol extractions, Proteinase K treatment, nor carrier RNA required
• No time-consuming ultracentrifugation, filtration nor special syringes required
• No precipitation reagents nor overnight incubation required
• Pure exosomes are purified and are free from any other RNA-binding proteins
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin
• The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services

Norgen’s Cell Culture Media Exosome Purification and RNA Isolation Kits constitute an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Cell Culture Media Exosome Purification and RNA Isolation Mini Kit

For sample input volumes ranging from 5 mL to 10 mL.

Cell Culture Media Exosome Purification and RNA Isolation Midi Kit

For sample input volumes ranging from 10 mL to 20 mL.

Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit

For sample input volumes ranging from 20 mL to 35 mL.

Details

Supporting Data

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Kit Specifications
Sample Type	Cell-Free Cell Culture Media
Sample Volume Range	10 mL to 20 mL
Size of RNA Purified	All sizes, including miRNA and small RNA (<200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	40-45 minutes
Average Yields*	Variable depending on specimen

*Please check page 4 of the product insert for Average Yields and Common RNA Quantification Methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60ºC if any salt precipitation is observed.

Component	Cat. 60700 (50 preps)	Cat. 60800 (25 preps)	Cat. 60900 (15 preps)
Slurry E	12.5 mL	12.5 mL	12.5 mL
ExoC Buffer	1.5 mL	1.5 mL	1.5 mL
ExoR Buffer	12 mL	12 mL	12 mL
Lysis Buffer A	20 mL	20 mL	20 mL
Lysis Additive B	2 mL	2 mL	2 mL
Wash Solution A	18 mL	18 mL	18 mL
Elution Solution A	6 mL	6 mL	6 mL
Mini Filter Spin Column	50	25	15
Mini Spin Columns	50	25	15
Collection Tubes	50	25	15
Elution tubes (1.7 mL)	100	50	30
Product Insert	1	1	1

Specification

7-50x    continuous    zoom    stereo    microscope		XTL-205A	XTL-205B	XTL-204A	XTL-2048	XTL-203A	XTL-203B
Zoom      Body	Binocular head,45°decline,360°rotate	●		●		●
	Trinocular head,45°decline,360*rotate		●		●		●
Eyepiece	WF10X22mm	●	●	●	●	●	●
Objective	zoom objective 0.7X—5X	●	●	●	●	●	●
Zoom     Rate	0.1:07.1	●	●	●	●	●	●
Total Amplific- ation   Rate	the standard configuration is 7X-50X	●	●	●	●	●	●
Auxiliary Lens	0.5X,0.7X,1X,1.5X,2X For Option	O	O	O	O	O	O
WorkingDistance	100mm	●	●	●	●	●	●
Diopter	55mm-75mm	●	●	●	●	●	●
Focus    arm	76mm Diameter,32mm pole size	●	●	●	●	●	●
Plate	Black/White plate,Glass plate for option	●	●	●	●	●	●
Optional	Stand	Q	Q
Stand	BI3 base.column sunnort unner and ower light source 3 WLED			●	●
	BL3-A base vertical arm bracket upper and lower light source 3 WLED	●	●
	B3 base column support has no light source					●	●
External light source	LED illumination	Q	Q	Q		O
Optiona	Photography,camera accessories CCD Coupler	o	o	o	o	o	o
Optional	Display attachment Monitor

Product Description

Fast RNA Extraction Reagent: High Efficiency, Reliable Results

Product Description:

Amp-future Bio’s Nucleic Acid Extraction Reagent is a RNA extraction kit that provides the highest quality nucleic acid extraction. The RNA extraction kit is an automated nucleic acid extractor which allows for quick and easy RNA isolation. The reagent is in liquid form and has a shelf life of 12 months, making it a reliable and durable product. With the Nucleic Acid Extraction Reagent, Amp-future Bio provides the accuracy and reliability of a professionally designed RNA extraction kit.

Features:

• Product Name: RNA Nucleic Acid Extraction Reagent
• Brand: Amp-future Bio
• Form: Liquid
• Shelf Life: 12 Months
• Application: RNA Extract
• Keywords:  RNA Extraction Kit 

Technical Parameters:

Property	Value
Form	Liquid
Product Name	RNA Nucleic Acid Extraction Reagent
Application	RNA Extraction
Brand	Amp-future Bio
Shelf Life	12 Months
Composition	5ml*4 tubes

Applications:

Amp-future Bio’s Nucleic Acid Reagent is an advanced automated nucleic acid extraction solution designed to efficiently extract RNA from biological samples while maintaining quality and accuracy. It is made in China and has a 12-month shelf life. It comes in a liquid form and is suitable for use in automated nucleic acid extractors, allowing for automatic and highly efficient extraction of nucleic acid from various sample types. The reagent is also designed to be easy to use, making it an ideal solution for laboratories that require fast and reliable results. This reagent is a great choice for any research or clinical laboratory that needs efficient and accurate nucleic acid extraction.

Support and Services:

Technical Support and Services for Nucleic Acid Reagent
We provide technical support and services for Nucleic Acid Reagents. Our experts are available to help you identify the right reagents for your application, answer any questions, and provide assistance on the use and optimization of these reagents. Our experts are also available to provide advice on troubleshooting and protocol optimization. We offer a range of services to make ordering and stocking of reagents easy and convenient.

We provide:

• Expert advice on the selection of the right reagents for your application
• Free samples for evaluation
• Bulk and custom orders
• Technical support and troubleshooting assistance
• Secure and reliable online ordering
• Fast and convenient shipping options

Please contact us for more information about our technical support and services for nucleic acid reagents.