The Norgen PCRSizer 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our PCRSizer contains ten discrete fragments ranging from 100 bp to 1000 bp in 100 bp increments with a higher intensity reference band at 500 bp. This Ladder is ideal for PCR product size confirmation.
Contents: 1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
PCR Sizer 100 bp DNA Ladder (Cat# 11400) – 100 loads
Ladder Properties: • Ten discrete bands, ranging from 100 bp to 1,000 bp • Higher intensity band at 500 bp for easy reference
Fragment
Size (bp)
Mass (ng)
1
1000
78
2
900
70
3
800
62
4
700
55
5
600
47
6
500
78
7
400
31
8
300
24
9
200
31
10
100
24
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Other Products
Blood Genomic DNA Isolation Mini Kit Dx
Product Info
Document
Product Info
Overview
CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
Input volumes up to 200 µL
High yield and high quality DNA ready for any application
Fast and convenient spin column protocol
Norgen’s Blood Genomic DNA Isolation Mini Kit Dx is designed for the rapid preparation of genomic DNA from up to 200 µL of whole blood for subsequent in vitro diagnostic use. Both fresh and frozen anticoagulated blood may be used with this procedure. Purification is based on spin column chromatography as the separation matrix. Norgen’s column binds DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions.
This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of DNA followed by signal detection or amplification. Any diagnostic results generated using the DNA isolated with Norgen’s Blood Genomic DNA Isolation Mini Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.
To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used.
Norgen’s Blood Genomic DNA Isolation Mini Kit Dx is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques including experience with whole blood samples and DNA isolation.
Norgen’s Blood Genomic DNA Isolation Mini Kit Dx does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.
*Yield will vary depending on the type of blood processed
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers. The kit contains a ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.
HiPure HP Plant RNA Mini Kit combines guanidine isothiocyanate lysing and silica gel membrane purification technology to simplify total RNA extraction. Ultracentrifugation in CsCl purification and LiCl / ethanol precipitation are not required. The kit uses DNase digestion to completely remove DNA. It is suitable for extracting up to 100μg of total RNA (including miRNA) from plant samples less than 200mg. Several samples can be extracted within 40 minutes. The purified RNA can be directly used for RT-PCR, fluorescent quantitative RT-PCR, Northern hybridization, second generation sequencing, etc.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA (include miRNA) from <200mg difficult-to-extract plant samples (use low toxicity chloroform substitutes)
Applications
RT-PCR, qPCR, Northern hybridization, second generation sequencing, nucleic acid protection, in vitro translation
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Hard-to-extraction plant samples such as fruit and seed, grape leaves, tea
Sample amount
≤200 mg
Elution volume
≥30μl
Time per run
1-24 samples within 30 minutes
Liquid carrying volume per column
800µl
Binding yield of column
100µg
Principle
This kit uses glass fiber filter membrane purification technique, and only requires simple combination-washing-elution steps. The sample is lysed and homogenized in the solution containing guanidine salt, ethanol is added to provide appropriate binding conditions, and transferred to the purification column for centrifugation. Up to 100µg of RNA can be selectively bound to the membrane, pollutants are efficiently washed off after three times of washing, and finally the purified RNA is eluted by RNase Free Water.
Advantages
Completely remove DNA by using of DNase
High quality – one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration
Fast – several samples can be extracted in 40 minutes by column method
Sensitive – RNA can be recovered at the level of PG
Broad spectrum – various types of plant samples can be processed by diversity of operating procedures
Kit Contents
Contents
R416502
D416503
Purification Times
50 Preps
250 Preps
HiPure RNA Mini Columns
50
250
2ml Collection Tubes
100
250
DNase I
600 μl
5 x 600 μl
DNase Buffer
6 ml
30 ml
Buffer PAL
60 ml
270 ml
Buffer GXP2*
20 ml
100 ml
Buffer BDP
60 ml
270 ml
Buffer RW1
50 ml
250 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml
Storage and Stability
DNase I should be stored at -20-8°C upon arrival. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Purchase Guide
1. When dealing with woody or uncommon samples, R4150 is recommended first. R4150 contains two polysaccharide/polyphenol lysis buffer, which is the most universal product.
2. R4151 is recommended for handling common economic crop samples for the first time. Strong lysis solution can be used to process easy-extraction samples. The amount of corn or rice leaves samples can reach up to 300mg.
3. R4165 adopts CTAB/chloroform method, which can also handle a large number of difficult-to-extraction plants, but requires contact with chloroform substitutes, which is less safe than other kits. This kit uses DNase Ⅰ to remove DNA, which is also a good choice for extracting polysaccharide/polyphenol-rich plant samples.
4. R4014 is recommended for fruit/starch plant samples, which uses improved trizol pre-treatment, single column operation and is more economical.
Select the right purification kit to get impactful results：
HiPure HP Plant RNA Mini Kit combines guanidine isothiocyanate lysing and silica gel membrane purification technology to simplify total RNA extraction. Ultracentrifugation in CsCl purification and LiCl / ethanol precipitation are not required. The kit uses DNase digestion to completely remove DNA. It is suitable for extracting up to 100μg of total RNA (including miRNA) from plant samples less than 200mg. Several samples can be extracted within 40 minutes. The purified RNA can be directly used for RT-PCR, fluorescent quantitative RT-PCR, Northern hybridization, second generation sequencing, etc.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
β-Glucan
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
510
Signal Response:
Increase
Linear Range:
4 to 100 μg of glucose per assay
Limit of Detection:
1 g/100 g
Total Assay Time:
~ 100 min
Application examples:
Yeast preparations, mushroom preparations and other materials.
Method recognition:
Novel method
The β-Glucan Assay Kit (Yeast and Mushroom) is suitable for the indirect measurement of 1,3:1,6-β-glucan in yeast and mushroom preparations containing starch, glycogen, sucrose and trehalose.
We are happy to announce a new and improved procedure!
Trehalase enzyme has now been added to the β-Glucan Assay Kit (Yeast and Mushroom), further increasing the accuracy of β-glucan measurement for certain samples. The addition of trehalase removes instances of overestimation of β-glucan content in samples containing trehalose. Please refer to the assay protocol and the FAQ here for more information.
This product replaces the Enzymatic Yeast β-Glucan Assay Kit (K-EBHLG) as the method of measurement for β-glucan measurement in yeast samples. See here for more information.
All reagents stable for > 12 months after preparation
Only enzymatic kit available
Simple format
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Document
The β-Glucan Assay Kit (Yeast and Mushroom) is suitable for the indirect measurement of 1,3:1,6-β-glucan in yeast and mushroom preparations containing starch, glycogen, sucrose and trehalose.
