PEG13-bis(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane) is large molecule with two sets of three terminal alkynes joined together by a large PEG linker. The alkynes are most frequently used in copper click chemistry with azides. The PEG linkers as well as numerous amide bonds provide high aqueous solubility to this compound, which may alter ADME of this compound.
PEG13-bis(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane) is large molecule with two sets of three terminal alkynes joined together by a large PEG linker. The alkynes are most frequently used in copper click chemistry with azides. The PEG linkers as well as numerous amide bonds provide high aqueous solubility to this compound, which may alter ADME of this compound.
MagPure Circulating DNA Mini Kit is designed for purification of high quality circulating DNA (cfDNA) fromcell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
Specifications
| Features | Specifications |
| Main Functions | Isolation circulating DNA from 0.2-0.6ml plasma, serum, body fluids |
| Applications | qPCR, NGS, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Serum, plasma |
| Sample amount | 0.2-0.6ml |
| Elution volume | ≥30μl |
| Time per run | ≤50 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.
| Contents | IVD5432 |
| Purification Times | 200 |
| MagPure Particles G | 14 ml |
| Carrier RNA | 310 μg |
| Proteinase K | 180 mg |
| Protease Dissolve Buffer | 10 ml |
| Buffer MLK | 250 ml |
| Buffer MAW1 | 250 ml |
| Buffer MW2* | 2 x 50 ml |
| Elution Buffer | 60 ml |
| Contents | IVD5432-F-96A | IVD5432-F-96B | IVD5432-F-96C |
| Sample amount | 200～350μl | 400~700μl | |
| Carrier RNA | 110 μg | 110 μg | |
| Proteinase K | 50 mg | 100 mg | |
| Protease Dissolve Buffer | 5 ml | 6 ml | |
| Elution Buffer | 15 ml | 15 ml | |
| Tip | 1 | 1 | |
| Sample Plate A | 500μl Buffer MLK | 500μl Buffer MLK | |
| Sample Plate B | / | 500μl Buffer MLK | |
| Wash Plate 1 | 700μl Buffer MAW1 | 700μl Buffer MAW1 | |
| Wash Plate 2 | 25μl Buffer MPG2700μl Buffer MW2 | 25μl Buffer MPG2700μl Buffer MW2 | |
| Wash Plate 3 | 700μl Buffer MW2 | 700μl Buffer MW2 | |
| Elution Plate | / | / |
| Contents | IVD5432-TL-06A | IVD5432-TL-06B | IVD5432-TL-06C |
| Sample amount | 300~350μl | 600~700μl | 900~1050μL |
| Carrier RNA | 310 μg | 310 μg | 310 μg |
| Proteinase K | 50 mg | 100 mg | 150 mg |
| Protease Dissolve Buffer | 6 ml | 6 ml | 10 ml |
| Elution Buffer | 15 ml | 15 ml | 15 ml |
| DA-Tip | 12 | 12 | 12 |
| Row 1/7 | 600μl Buffer MLK | 600μl Buffer MLK | 600μl Buffer MLK |
| Row 2/8 | / | 600μl Buffer MLK | 600μl Buffer MLK |
| Row 3/9 | 600μl Buffer MAW1 | 600μl Buffer MAW1 | 600μl Buffer MLK |
| Row 4/10 | 20μl Buffer MPG2600μl Buffer MW2 | 20μl Buffer MPG2600μl Buffer MW2 | 30μl Buffer MPG2900μl Buffer MAW1 |
| Row 5/11 | 600μl Buffer MW2 | 600μl Buffer MW2 | 900μl Buffer MW2 |
| Row 6/12 | / | / | / |
Storage and Stability
Proteinase K, Carrier RNA and MagPure Particles G should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should beredissolved before use. Make sure that all buffers are at room temperature when used.
MagPure Circulating DNA Mini Kit is designed for purification of high quality circulating DNA (cfDNA) fromcell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
Details
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected*. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
The PCR-free NGS DNA Library Prep Kit was developed for construction of DNA libraries for next generation sequencing (illumina platform). The kit adds 3′-dT-tailed library adapters to both ends of DNA fragments efficiently. The kit uses double strand DNA fragments (blunt and/or sticky) as input DNA for NGS library construction, and is compatible with DNA fragments generated from both enzymatic methods (BioDynami DNA fragmentation enzymes etc.) and physical methods (sonication, nebulization etc.).
PCR-Free NGS DNA Library Prep Kit Workflow
PCR amplification is a standard step for library preparation of Next-Generation Sequencing. The PCR is used to amplify fully ligated DNA fragments and to add index information to the libraries. The indexing is for the pooling of the library samples in an effort to reduce the sequencing cost.
However, PCR introduces uneven amplification of DNA in some DNA regions with extreme GC-contents and secondary structures. This bias can cause very low sequencing coverage in such regions. DNA sequencing in these regions is still a huge challenge.
PCR-free library prep can reduce library bias and minimize sequencing gaps. The sequencing data from PCR-free library samples have even genomic coverage with few gaps and better depth in GC-rich regions. Our PCR-free NGS kit offers optimal coverage in the regions that are traditionally difficult, such as high-GC content regions, low-GC content regions, and repetitive sequence regions.
Two index types are available for the kit:
Non-index: Libraries do not have index.
Index: Each library contains one i5 index and one i7 index. Library multiplexing up to 96 samples is possible. List of indexes can be downloaded Here.
Kit advantages:
The PCR-free NGS DNA Library Prep Kit was developed for construction of DNA libraries for next generation sequencing (illumina platform). The kit adds 3′-dT-tailed library adapters to both ends of DNA fragments efficiently. The kit uses double strand DNA fragments (blunt and/or sticky) as input DNA for NGS library construction, and is compatible with DNA fragments generated from both enzymatic methods (BioDynami DNA fragmentation enzymes etc.) and physical methods (sonication, nebulization etc.).
