Propargyl-PEG8-t-butyl ester is a PEG reagent with an alkyne group and a t-butyl group. The alkyne group enables the formation of triazole linkage with azide-bearing compounds or biomolecules; copper is required as a catalyst. The t-butyl group can be hydrolyzed under acidic conditions. The hydrophicility of the molecule is improved by having 8 units of PEG. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG8-t-butyl ester is a PEG reagent with an alkyne group and a t-butyl group. The alkyne group enables the formation of triazole linkage with azide-bearing compounds or biomolecules; copper is required as a catalyst. The t-butyl group can be hydrolyzed under acidic conditions. The hydrophicility of the molecule is improved by having 8 units of PEG. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Introduction

The RNA Clean Beads use paramagnetic bead technology for high-throughput purification of RNA or cDNA from in vitro applications such as transcription, antisense RNA (aRNA) amplification and RNA and cDNA probe synthesis. The resulting purified product can be used in the following applications: PCR and RT-PCR, probes for microarray or macroarray, RNase protection assays, transfection for RNAi experiments and cDNA synthesis and labeling.

Details

Specifications

Features	Specifications
Main Functions	Recovery of RNA from RNA reaction solution by magnetic bead method (Replace Beckmen RNAClean XP)
Applications	Advanced applications, such as sequencing, genechip, fluorescence quantification, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	RNA products, restriction endonuclease systems, or other enzymatic reaction solutions
Sample amount	20-100µl
Recovery	90%
Elution volume	≥20μl
Operation time	≤50 minutes

Principle

RNA Clean utilizes an optimized buffer to selectively bind RNA or cDNA to paramagnetic beads. Excess oligonucleotides, nucleotides, salts, and enzymes can be removed using a simple washing procedure.

Advantages

• High recovery – recovery of RNA up to 90%
• High throughput – using magnetic beads purification technology
• Simple – simplified operation process, combination – washing – elution

Kit Contents

Contents	BRP-5	BRP-50	BRP-500
RNA Clean Beads	5 ml	50 ml	500 ml

Storage and Stability

Store at 4℃ upon arrival, for up to 12 months. For best results shake the reagent well until all of the beads are completely in suspension and aliquot RNAClean Beads into RNase free containers. Do not pour remaining reagent back into the storage container. Mix RNAClean Beads well before use. The reagent should appear homogenous and consistent in color.

DO NOT FREEZE.

The RNA Clean Beads use paramagnetic bead technology for high-throughput purification of RNA or cDNA from in vitro applications such as transcription, antisense RNA (aRNA) amplification and RNA and cDNA probe synthesis. The resulting purified product can be used in the following applications: PCR and RT-PCR, probes for microarray or macroarray, RNase protection assays, transfection for RNAi experiments and cDNA synthesis and labeling.

 Features 

1.Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.  

2.Imported compressors fluorine free,double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control.

3.Microprocessor control, digital display which indicates the temperature, speed, time, RCF in operation, speed raising and reducing quickly, operate simply. Running parameter can be edited.

4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.

5. Automatically electric lid lock, over speed, over temperature protection and imbalance protection.

6.High quality steel centrifuge body , stainless steel chamber, built-in stainless steel explosion-proof protect sleeve , 3 tiers protection,safe and reliable.

7. Rotor is connected to spindle by specialized spring taper sleeve, loading simple and quick, no direction

8. With pre-cooling and get the ideal centrifugation result.

 TGL16 Technical Parameter：

Max. Speed	16000rpm
Max. RCF	20600×g
Max. Capacity	6×100ml
Time Range	1~9h59min
RPM/RCF Convert	Yes
Noise (dB)	≤ 58
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	±1℃
Voltage(V/Hz)	AC 220V/110V  50HZ/60HZ
Size (L x W x Hmm)	610×570×370mm
Net Weight(Kg)	82KG
Certificates	TUV CE,ISO 9001,ISO13485 & Calibration report are available

 Matched Rotor for TGL16 

Order No	Rotor	Max speed (rpm)	Max Volume(ml)	Max RCF (g)
G16-1	Angle rotor	14000	4×8PCR	12070
G16-2	Angle rotor	13000	6×8PCR	16080
G16-3	Angle rotor	14000	8×8PCR	13390
G16-4	Angle rotor	13000	12×8PCR	17220
G16-5	Angle Rotor	15000	30×0.5ml	18510
G16-6	Angle Rotor	14000	40×0.5ml	19970
G16-7	Angle Rotor	16000	12×1.5/2ml	17940
G16-8	Angle Rotor	15000	24×1.5/2ml	20600
G16-9	Angle Rotor	13500	30×1.5/2ml	19340
G16-10	Angle Rotor	11000	48×1.5/2ml	12840
G16-11	Angle Rotor	15000	16×5ml	19350
G16-12	Angle Rotor	14000	12×7ml	16370
G16-13	Angle Rotor	14000	6×10ml	16460
G16-14	Angle Rotor	12000	12×10ml	14510
G16-15	Angle Rotor	10000	12×15ml	11840
G16-16	Angle Rotor	5000	24×15ml	3080
G16-17	Angle Rotor	5000	30×15ml	3830
G16-18	Angle Rotor	12000	8×20ml	14510
G16-19	Angle Rotor	12000	6×30ml	14000
G16-20	Angle Rotor	11000	6×50ml	13480
G16-21	Angle Rotor	12000	6×70ml	10810
G16-22	Angle Rotor	8000	6×100ml	7280
G16-23	Angle Rotor	12000	24 pieces capillary vessel	15800
G16-24	Microplate rotor	4000	2×3×48 Well	2300