PEG13-bis(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane) is large molecule with two sets of three terminal alkynes joined together by a large PEG linker. The alkynes are most frequently used in copper click chemistry with azides. The PEG linkers as well as numerous amide bonds provide high aqueous solubility to this compound, which may alter ADME of this compound.
PEG13-bis(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane) is large molecule with two sets of three terminal alkynes joined together by a large PEG linker. The alkynes are most frequently used in copper click chemistry with azides. The PEG linkers as well as numerous amide bonds provide high aqueous solubility to this compound, which may alter ADME of this compound.
This kit is suitable for the isolation of total RNA from a range of animal tissues such as liver and spleen, as well as difficult fibrous tissues such as heart, muscle, intestine, etc. Briefly, the tissue of interest is first lysed using Buffer RL, followed by treatment with the provided Proteinase K which aids in the removal of the various proteins present in fiber-rich tissues including collagen, contractile proteins, and connective tissues. The purified RNA is of the highest quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS, and more.
The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
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| Kit Specifications | |
| Maximum Column Binding Capacity | 50 μg |
| Maximum Column Loading Volume | 650 μL |
| Size of RNA Purified | All sizes, including small RNA < 200 nt |
| Time to Complete 10 Purifications | 50 minutes |
| Average Yield Liver (10mg) Kidney (10mg) Spleen (10mg) Heart (10mg) Muscle (10mg) | 30 μg 25 μg 15 μg 10 μg 5 μg |
| Maximum Amount of Starting Material Heart Kidney Liver Muscle Spleen | 30 mg 15 mg 15 mg 30 mg 15 mg |
Storage Conditions and Product Stability
The DNAse I should be stored at -20°C upon arrival. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date shipment.
| Component | Cat. 25700 (50 preps) |
|---|---|
| Buffer RL | 30 mL |
| RNase-Free Water | 40 mL |
| Wash Solution A | 38 mL |
| Enzyme Incubation Buffer A | 6 mL |
| Elution Solution A | 6 mL |
| Proteinase K | 2 vials |
| DNase I | 1 vial |
| Mini Spin Columns | 50 |
| Collection Tubes | 100 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
Bis-propargyl-PEG6 has two propargyl groups which can participate in Click Chemistry reactions to yield a stable triazole linkage with azide compounds; copper is needed as a catalyst. The 6 units of PEG increase the hydrophilicity of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Bis-propargyl-PEG6 has two propargyl groups which can participate in Click Chemistry reactions to yield a stable triazole linkage with azide compounds; copper is needed as a catalyst. The 6 units of PEG increase the hydrophilicity of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
