PEG3-bis(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane) is a crosslinker consisting of six propargyl groups. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose.
Detail
PEG3-bis(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane) is a crosslinker consisting of six propargyl groups. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose.
Other Products
[DL4000] ExcelDye™ 6X DNA Loading Dye, Tri-color, 5 ml x 2
Product Info
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Product Info
Description
The ExcelDye™ 6× DNA Loading Dye (Tri-Color) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains three dyes (Xylene cyanol FF, Bromophenol blue, Orange G) for tracking the DNA migration. The Xylene cyanol FF, Bromophenol blue and Orange G migrate at approximately 800 bp, 150 bp and 30 bp on a standard 2% TAE agarose gel respectively (4,000 bp, 500 bp and 50 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
Composition
0.03% Xylene cyanol FF
0.03% Bromophenol blue
0.15% Orange G
10 mM Tris-HCl (pH 8.0)
60% glycerol
60 mM EDTA
Storage
4°C for 12 months -20°C for 36 months
Document
The ExcelDye™ 6× DNA Loading Dye (Tri-Color) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains three dyes (Xylene cyanol FF, Bromophenol blue, Orange G) for tracking the DNA migration. The Xylene cyanol FF, Bromophenol blue and Orange G migrate at approximately 800 bp, 150 bp and 30 bp on a standard 2% TAE agarose gel respectively (4,000 bp, 500 bp and 50 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
[PM1600] ExcelBand™ All Blue Regular Range Plus Protein Marker (9-180 kDa), 250 μl x 2
Product Info
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Product Info
Description
The PM1600 ExcelBand™ All Blue Regular Range Plus Protein Marker is a blue protein standard with 11 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa, respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-Glycine buffer).
The PM1600 ExcelBand™ All Blue Regular Range Plus Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Features
Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.
Two enhanced bands — 72 kDa and 25 kDa
Contents
Approximately 0.1~0.5 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol).
Quality Control
Under suggested conditions, PM1600 ExcelBand™ All Blue Regular Range Plus Protein Marker resolves 11 major bands in 15% SDS-PAGE (Tris-Glycine buffer, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane.
Storage
4°C for 3 months -20°C for long term storage
Document
The PM1600 ExcelBand™ All Blue Regular Range Plus Protein Marker is a blue protein standard with 11 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa, respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-Glycine buffer).
The PM1600 ExcelBand™ All Blue Regular Range Plus Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from tissue / blood / body fluid / swab /dry blood spots
Applications
PCR, qPCR, southern bolt and virus detection, etc
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Tissue, cell, blood, saliva, swab, blood spot,semen and other clinical samples
Sample amount
Solid tissue: 1-10mg; Anticoagulant blood: 200μl
Elution volume
≥20μl
Time per run
30 – 60 minutes
Liquid carrying volume per column
800µl
Binding yield of column
100µg
Principle
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
Fast – without separation of leukocytes, organic extraction or ethanol precipitation
Simple – all nucleic acids can be obtained by direct digestion
Wide applicability- handle a variety of liquid samples
Kit Contents
Contents
D301802
D301803
Purification Times
50
250
HiPure DNA Mini Columns I
50
250
2ml Collection Tubes
100
500
Buffer ATL
30 ml
150 ml
Buffer AL
30 ml
150 ml
Buffer GW1
22 ml
88 ml
Buffer GW2
12 ml
50 ml
Proteinase K
24 mg
120 mg
Protease Dissolve Buffer
1.8 ml
10 ml
Buffer AE
15 ml
60 ml
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Experiment Data
Document
This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.