PEG3-bis(Amino-Tri-(Propargyl-PEG8-ethoxymethyl)-methane) is large molecule with two sets of three terminal alkynes joined together by a short PEG linker. The alkynes are most frequently used in copper click chemistry with azides to form stable triazoles with the target compound. The PEG linkers as well as numerous amide bonds provide high aqueous solubility to this compound, which may alter DMPK of this compound.
PEG3-bis(Amino-Tri-(Propargyl-PEG8-ethoxymethyl)-methane) is large molecule with two sets of three terminal alkynes joined together by a short PEG linker. The alkynes are most frequently used in copper click chemistry with azides to form stable triazoles with the target compound. The PEG linkers as well as numerous amide bonds provide high aqueous solubility to this compound, which may alter DMPK of this compound.
Designed for those labs wishing to separate magnetic beads into rings.
The Permagen ring magnet low elution plate was designed for use in automation applications where volumes as low as 5 µL are required. Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added an angled frame around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets during automation
SBS SLAS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
RLE500
Maximum – 150 µL
Minimum – 5 µL
Designed for those labs wishing to separate magnetic beads into rings.
The Permagen ring magnet low elution plate was designed for use in automation applications where volumes as low as 5 µL are required. Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added an angled frame around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets during automation
These kits provide a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples, including those preserved using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. Purifies the DNA with high yields and molecular weights of up to 50 kb plus. The purified DNA can be used with a number of downstream applications.
Stool DNA Isolation Kit (Spin Column)
Universal method to detect microorganism and host cell DNA simultaneously in stool samples. Eliminates PCR inhibitors including all traces of humic acid using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.
Stool DNA Isolation 96-Well Kit (High Throughput)
Fast and easy high throughput processing using centrifugation. Eliminates PCR inhibitors including all traces of humic acid using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.
Stool DNA Isolation Kit (Magnetic Bead System)
Fast and easy processing using a magnetic bead system. Robust lysis system (chemical lysis combined with a mechanical homogenization).
Stool DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
This kit provides fast and easy processing using a magnetic bead system, and a robust lysis system (chemical lysis combined with a mechanical homogenization). High throughput and compatible with an automation robotic system.
Free Download
Tips and tricks for isolating high yield and quality DNA, RNA, miRNA and EV’s from fecal samplesDownload for Free
Figure 1 / 12
Click for expanded view
| Kit Specifications | |
| Maximum Stool Input | 200 mg (fresh/frozen stool) or 400 μL (preserved stool) |
| Type of Stool Processed | Frozen, fresh or preserved stool |
| Format | Spin Column |
| Maximum Column Binding Capacity | 50 μg |
| Maximum Column Loading Volume | 650 μL |
| Elution Volume | 50 μL |
| Time to Complete 10 Purifications | 30 minutes |
| Applications | PCR, qPCR, Southern Blot Analysis, Sequencing, Microarray Analysis. |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 27600 (50 preps) | Cat. 65600 (192 preps) | Cat. 55700 (50 preps) | Cat. 63100 (192 preps) |
|---|---|---|---|---|
| Lysis Buffer L | 60 mL | 2 x 105 mL | 60 mL | 3 x 60 mL 1 x 30 mL |
| Lysis Additive A | 6 mL | 25 mL | 6 mL | 25 mL |
| Binding Buffer I | 7 mL | 25 mL | 7 mL | 25 mL |
| Binding Buffer C | 30 mL | 110 mL | – | – |
| Wash Solution A | 18 mL | 2 x 38 mL | – | – |
| Magnetic Bead Suspension | – | – | 1.1 mL | 4 x 1.1 mL |
| Solution WN | – | – | 18 mL | 55 mL |
| Elution Buffer B | 8 mL | 30 mL | 8 mL | 30 mL |
| Bead Tubes | 50 | 200 | 50 | 196 |
| Mini Spin Columns | 50 | – | – | – |
| 96-Well Plate | – | 2 | – | 2 |
| Adhesive Tape | – | 4 | – | 2 |
| Collection Tubes | 50 | – | – | – |
| 96-Well Collection Plate | – | 2 | – | – |
| Elution Tubes (1.7 mL) | 50 | – | 50 | – |
| 96-Well Elution Plate | – | 2 | – | 2 |
| Product Insert | 1 | 1 | 1 | 1 |
