Description
This kit can be used in quantitative and qualitative analysis of Pentachlorophenol residue in honey, milk, juice, and rice.
This kit is based on ELISA technology, which is fast, easy, accurate and sensitive compared with common instrumental analysis and only needs 1.5 hours in one operation, it can considerably minimize operation error and work intensity.
This kit can be used in quantitative and qualitative analysis of Pentachlorophenol residue in honey, milk, juice, and rice.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
Norgen’s Saliva DNA Isolation Kit (Magnetic Bead System) provides a fast and reproducible method for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva. Saliva DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream research applications including PCR, Southern Blot analysis, sequencing and microarray analysis.
Norgen’s Saliva DNA Isolation Kit (Magnetic Bead System) is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
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| Kit Specifications | |
| Maximum Saliva Input | 0.5 mL preserved saliva 0.25 mL fresh saliva |
| Average Yield from 0.25 mL of Saliva* | 2-5 μg |
| Average Purity (OD260/280) | 1.6 – 1.8 |
| Time to Complete 10 Purifications | 30 minutes (Cat. RU55400) 45 minutes (Cat. RU62900) |
* Average DNA yield will vary depending on the donor
Storage Conditions and Product Stability
The kit contains ready-to-use Proteinase K which is dissolved in a specially prepared storage buffer. The Proteinase K should be stored at room temperature or 4°C. All other solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). These reagents should remain stable for at least 2 years in their unopened containers.
| Component | Cat. RU55400 (50 preps) | Cat. RU62900 (192 preps) |
|---|---|---|
| Lysis Buffer F | 30 mL | 2 x 30 mL |
| Proteinase K in Storage Buffer | 1.2 mL | 4 mL |
| Magnetic Bead Suspension | 2 x 1.1 mL | 8.5 mL |
| Elution Buffer B | 8 mL | 30 mL |
| Elution Tubes (1.7 mL) | 50 | – |
| 96-Well Plate | – | 2 |
| 96-Well Elution Plate | – | 2 |
| Adhesive Tape | – | 2 |
| Product Insert | 1 | 1 |
N-(Propargyl-PEG2)-PEG3-t-butyl ester is a branched PEG linker with an alkyne group and a t-butyl ester. The alkyne group reacts with azide-bearing compound in coppper catalyzed azide-alkyne Click Chemistry reaction. The amino group is reactive with carboxylic acids, activated NHS esters, and carbonyls. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
N-(Propargyl-PEG2)-PEG3-t-butyl ester is a branched PEG linker with an alkyne group and a t-butyl ester. The alkyne group reacts with azide-bearing compound in coppper catalyzed azide-alkyne Click Chemistry reaction. The amino group is reactive with carboxylic acids, activated NHS esters, and carbonyls. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.