K-PHOS
SKU: 700004326
100 assays (manual) / 400 assays (auto-analyser)
| Content: | 100 assays (manual) / 400 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Phosphate |
| Assay Format: | Spectrophotometer, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 360 |
| Signal Response: | Increase |
| Linear Range: | 0.1 to 10 μg of phosphate per assay |
| Limit of Detection: | 0.16 mg/L |
| Reaction Time (min): | ~ 20 min |
| Application examples: | Processed foods and drinks, bakery products, dairy products, waste water samples, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, etc.). |
The Phosphate Assay Kit is a rapid, simple, reliable and accurate method for the specific measurement and analysis of phosphate in water, foodstuffs, beverages and other materials. The phosphate detection reaction employs the defined chemical substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG). In the presence of phosphate, the substrate is converted enzymatically by purine nucleoside phosphorylase (PNPase) to the products ribose 1-phosphate and 2-amino-6-mercapto-7-methylpurine. An application note describes the dephosphorylation reaction of phytic acid using phytase and alkaline phosphatase (sold separately), and subsequent phosphate detection using the reagents provided in K-PHOS.
This method is suitable for analysis using spectrophotometer and auto-analyser.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.
Need other products? See our complete list of assay kits.
Advantages
- Chemically defined detection method
- All reagents stable for > 2 years after preparation
- Rapid reaction (~ 20 min)
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Suitable for manual and auto-analyser formats
