[PM2610] ExcelBand™ Enhanced 3-color High Range Protein Marker (9-245 kDa), 250 μl x 2
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The PM2610/PM2611 ExcelBand™ Enhanced 3-color High Range Protein Marker is a ready to use three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine buffer (9 to 235 kDa in BisTris (MOPS) buffer and 10-235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with two reference proteins carrying enhanced intensity corresponding to a green at 25 kDa and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The ExcelBand™ Enhanced 3-color High Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Detail
Description
The PM2610/PM2611 ExcelBand™ Enhanced 3-color High Range Protein Marker is a ready to use three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine buffer (9 to 235 kDa in BisTris (MOPS) buffer and 10-235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with two reference proteins carrying enhanced intensity corresponding to a green at 25 kDa and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The ExcelBand™ Enhanced 3-color High Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Features
Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.
Two reference bands — 75 kDa (red) and 25 kDa (green)
Contents
Approximately 0.2~0.6 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 3.6 M Urea, and 15 % (v/v) Glycerol).
Quality Control
Under suggested conditions, the PM2610/PM2611 Enhanced 3-color High Range Protein Marker resolves 12 major bands in 15% SDS-PAGE (Tris-Glycine buffer, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane.
Storage
4°C for 3 months -20°C for long term storage
Other Products
TG18 Tabletop 21000rpm High Speed Low Speed Non-refrigerated Large Capacity Multi-purpose lab centrifuge 800ml /500ml bottles & 5/7/10/15/50ml tubes
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Product Info
TG18 Tabletop 21000rpm High Speed Low Speed Non-refrigerated Large Capacity Multi-purpose lab centrifuge 800ml /500ml bottles & 5/7/10/15/50ml tubes. And holding Angle Rotors like 24×1.5/2ml, 6x50ml, 12x15ml…in high speed separation smooth.
It’s widely used in the field of animal and plant molecular biology, which is the ideal instrument for seperating the chemical substances in the cell and purifing the microorganisms, viruses, bacteira and subcelluar etc.
TG18 High Speed/Low Speed Centrifuge Features:
1. Max. Speed 21000rpm, max. volume 4x800ml, can be used for 5ml/10ml/15ml/30ml/50ml/100ml centrifuge tubes.
2. Brushless DC motor in great torque, no powder pollution, free maintenance, quick in speed up and down.
3. Can store 10 programs, automatically calculate and synchronously display the RCF data. Digital display indicates the speed, time, temperature and RCF. The parameters can be changed in operation, no need to stop the centrifuge which is quite convenient.
4. The centrifuge body is made of high-quality steel, and with high-quality steel centrifuge chamber, safe and reliable.
5. Automatically electric lid lock, over speed/over temperature protection and imbalance protection.
6. There are many kinds of rotors for your choice, both in high speed and low speed.
TG18 High Speed/Low Speed Centrifuge Technical Parameters:
Max. Speed
21000rpm
Max. RCF
30910xg
Max. Capacity
4x800ml
Timer
0-99min
Speed Accuracy
±20rpm
Noise(DB)
≤65DBA
Voltage(V/HZ)
AC220V/110V,50HZ 10A
Dimension(LxWxHmm)
685x500x385mm
Net Weight(Kg)
70KG
Certificates
CE, ISO
Warranty
1 Year
TG18 High Speed/Low Speed Centrifuge Matched Rotors:
Order No.
Rotor Type
Max Speed(r/min)
Volume(ml)
Max. RCF(xg)
G18-1
Swing Rotor
4000
4x800ml
3450
G18-2
Microplate Rotor
4000
4x4x96well
2940
G18-3
Microplate Rotor
4000
2x4x96well
3210
G18-4
Microplate Rotor
4000
2x3x48well
2300
G18-5
Swing Rotor
4000
4x30x5ml vacuum tube
2840
G18-6
4x30x7ml vacuum tube
3140
G18-7
4x18x10ml vacuum tube
3140
G18-8
Swing Rotor
4000
Fat bottle
3830
G18-9
Fixed Rotor
16000
4x8PCR
15760
G18-10
Fixed Rotor
15000
6x8PCR
21420
G18-11
Fixed Rotor
16000
8x8PCR
17480
G18-12
Fixed Rotor
15000
12x8PCR
22930
G18-13
Fixed Rotor
15000
40×0.5ml
22920
G18-14
Fixed Rotor
21000
12×1.5/2ml
30910
G18-15
Fixed Rotor
16000
24×1.5/2ml
23440
G18-16
Fixed Rotor
14000
30×1.5/2ml
20800
G18-17
Fixed Rotor
13000
48×1.5/2ml
17930
G18-18
Fixed Rotor
16000
16x5ml
22020
G18-19
Fixed Rotor
16000
6x10ml
21500
G18-20
Fixed Rotor
15000
12x10ml
22680
G18-21
Fixed Rotor
13000
16x10ml
19490
G18-22
Fixed Rotor
13000
8x15ml
17790
G18-23
Fixed Rotor
11000
12x15ml
14330
G18-24
Fixed Rotor
5000
24x15ml
3500
G18-25
Fixed Rotor
5000
30x15ml
3830
G18-26
Fixed Rotor
14000
6x30ml
19060
G18-27
Fixed Rotor
13000
6x50ml(conical)
18840
G18-28
Fixed Rotor
13000
6x50ml(round)
18730
G18-29
Fixed Rotor
5000
12x50ml
3860
G18-30
Fixed Rotor
4000
24x50ml
2970
G18-31
Fixed Rotor
13000
4x85ml
18940
G18-32
Fixed Rotor
12000
4x100ml
14850
G18-33
Fixed Rotor
10000
6x100ml
11380
G18-34
Fixed Rotor
4000
12x100ml
2970
G18-35
Fixed Rotor
12000
24 capillaries
15800
G18-36
Swing Rotor
15000
4x5ml
19920
G18-37
Vertical Rotor
16000
16x5ml
16540
G18-38
Vertical Rotor
14000
8x30ml
19750
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Disulfide Biotin alkyne is an azide-activated cleavable biotin probe that allows for efficient recovery of avidin-bound protein complexes in affinity-based assays. This reagent contains a biotin moiety linked to an alkyne group through a spacer arm containing a cleavable disulfide linker. Under reducing conditions (50 mM dithiothreitol, 10 mM 2-mercaptoethanol or 1% sodium borohydride), the disulfide bonds are cleaved, releasing the biotin tag and any avidin conjugate bound to it.
Document
Disulfide Biotin alkyne is an azide-activated cleavable biotin probe that allows for efficient recovery of avidin-bound protein complexes in affinity-based assays. This reagent contains a biotin moiety linked to an alkyne group through a spacer arm containing a cleavable disulfide linker. Under reducing conditions (50 mM dithiothreitol, 10 mM 2-mercaptoethanol or 1% sodium borohydride), the disulfide bonds are cleaved, releasing the biotin tag and any avidin conjugate bound to it.
Preserved Blood RNA Purification Kit II (for use with PAXgene Blood RNA Tubes)
Product Info
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Product Info
Overview
Compatible with PAXgene Blood RNA Tubes
Isolate true total RNA including siRNA and microRNA
No phenol extractions
Purify high-quality RNA in 30 minutes
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s Preserved Blood RNA Purification Kit II provides a rapid method for the isolation and purification of total RNA from blood that has been preserved using PAXgene Blood RNA Tubes*. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform.
Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Norgen’s proprietary resin provides superior affinity to the full size range of RNA molecules, resulting in large and small RNA (miRNA) purification with better linearity and sensitivity. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time RT-PCR, RT-PCR, Northern blotting, RNase protection and primer extension, expression profiling, miRNA cloning and amplification and Next Generation Sequencing.
* PAXgene Blood RNA Tubes are a product of PreAnalytiX GmbH. PAXgene Blood RNA Tubes are not provided.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The DNase I should be stored at -20°C.
