Portable 4000rpm TD4C Centrifuge Machine for Lab, PRP & PRF 

 K-MRH2O2

SKU: 700004322

1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)

This product has been discontinued.

The hydrogen peroxide Assay Kit provides a simple robust method for the measurement of hydrogen peroxide. This kit utilizes a highly sensitive Megaplex Red probe which in the presence of horseradish peroxidase (HRP), allows for the measurement of H₂O₂ in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation in the range 530-560 nm and emission at ~ 590 nm or colourimetrically at 570 nm. The excitation and emission maxima of Megaplex Red are 571 nm and 585 nm respectively.

Display all assay test kits available.

Fluorometric/UV method for the determination of H₂O₂ content in a variety of samples.

Advantages

• Simple format
• Very competitive price (cost per test)
• Ability to run in fluorescence or absorbance mode
• User friendly – Detailed protocol provided for the creation of calibration curve and the calculation of concentration in fluorescence mode. Single point standard for quicker and simpler analysis in absorbance mode.
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
• Suitable for manual, microplate and auto-analyser formats
• All reagents stable for > 2 years

The hydrogen peroxide Assay Kit provides a simple robust method for the measurement of hydrogen peroxide. This kit utilizes a highly sensitive Megaplex Red probe which in the presence of horseradish peroxidase (HRP), allows for the measurement of H2O2 in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation in the range 530-560 nm and emission at ~ 590 nm or colourimetrically at 570 nm. The excitation and emission maxima of Megaplex Red are 571 nm and 585 nm respectively.

Preferred by most scientists, the ring magnet plate is a versatile product that can be used manually or in automated liquid handlers when separating beads into rings is essential.

Available in three magnet strengths like our U500 above

Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads

SKU #

S380 / S480 / S500

Features

• SBS/ SLAS footprint
• Solid Aluminum alloy design
• Precision manufactured for more consistent results
• Fast magnetic bead separations
• 4 mm bead free ring at bottom of each well for easy removal
• Made in USA 
• For Research use only

Compatibility

• Round bottom plates 
• Pyramid bottom plates 
• PCR Plates
• S380 – Up to 350 µL volumes
• S480 – Up to 1000 µL volumes
• S500 – Up to 2000 µL volumes
• Any magnetic beads
• Any common liquid handlers i.e. Tecan®, Opentrons®, Hamilton®, Agilent®, Beckman®, etc.

Volumes

U380 – Maximum – 350 µL 

Minimum – 30 µL 

U480 – Maximum – 1 mL 

Minimum – 30 µL  

U500 – Maximum – 2 mL

Minimum – 30 µL 

Ideal magnet plate for those wishing to replace Beckman® PN: A32782. (50% Cost Savings*) Features all the same dimensions and even the same magnets. Does not include the spring base but it is the same height. Perfect for those using robots that do not require springs.

Product Description

High Sensitivity Isothermal PCR Kit NFO | Specificity, Short Reaction Time | 30-37℃ | 10-100 CFU/reaction

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Isothermal nucleic acid Applications

Suitable for DNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.