Introduction

A highly efficient, easily automated agarose gel or PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. The Kit can be easily used in manual and automated 96 or 384-well formats.

Details

Specifications

The Kit method contains magnetic particles in an optimized binding buffer to selectively bind DNA fragments (>100bp) and larger to paramagnetic beads. Excess primers, nucleotides, salts, and enzymes can be removed using a simple washing procedure.

Advantages

• High recovery efficiency – up to 80% DNA recovery
• General – recover DNA from gel or enzyme-driven reaction solutions such as PCR

Kit Contents

Storage and Stability

MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Experiment Data

A highly efficient, easily automated agarose gel or PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. The Kit can be easily used in manual and automated 96 or 384-well formats.

Name of Product

Trichinella spiralis – IgG ELISA

Catalog Number

9750

Short Info

Trichinellosis is caused by Trichinella spiralis, a parasitic nematode of pork, horse and wild animals. Humans can be infected by eating raw or undercooked meat from infected animals. Most infected people do not show any symptoms. However, in some cases, symptoms appear during intestinal stage (diarrhea and abdominal pain) and muscular stage (muscle pain, fever, edema). Diagnosis is based on signs and symptoms, but in many cases it is not conclusive for the diagnosis. Serological assays have an important place on the final diagnosis of the disease. The ELISA kit has shown appropriate performance for the serological screening of trichinellosis.

This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.

Method/Platform

ELISA in microplate format

Range/Assay Sensivity

95% sensitivity, 98% specificity

Test Principle

The kit provides all the material needed to perform 96 enzyme-linked immunosorbent assays (ELISA) on breakable microtitration wells sensitized with Trichinella spiralis excreted/secreted (E/S) larval antigens. Specific antibodies in the sample will bind to these antigens and washing will remove unspecific antibodies. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate. A second washing step will remove unbound conjugate. Revealing bound antibodies is made by the addition of pNPP substrate which turns yellow in the presence of alkaline phosphatase. Color intensity is proportional to the amount of Trichinella spiralis specific antibodies in the sample. Potassium phosphate is added to stop the reaction. Absorbance at 405 nm is read using an ELISA microplate reader. The test can be performed with automatic systems, but this must be validated by the user.

Brief Instructions

Storage

2-8° C

Components

ELISA wells, dilution buffer, washing solution, control sera, conjugate, substrate solution, stopping solution

Trichinellosis is caused by Trichinella spiralis, a parasitic nematode of pork, horse and wild animals. Humans can be infected by eating raw or undercooked meat from infected animals. Most infected people do not show any symptoms. However, in some cases, symptoms appear during intestinal stage (diarrhea and abdominal pain) and muscular stage (muscle pain, fever, edema). Diagnosis is based on signs and symptoms, but in many cases it is not conclusive for the diagnosis. Serological assays have an important place on the final diagnosis of the disease. The ELISA kit has shown appropriate performance for the serological screening of trichinellosis.