Portable Low Speed Centrifuge 

Overview

• Ensure optimal results for sensitive applications like NGS
• Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
• The purified exosomal RNA is free from any circulating RNA-binding proteins.
• No phenol extractions, Proteinase K treatment, nor carrier RNA.
• No time-consuming ultracentrifugation, filtration nor special syringes are required.
• Concentrate isolated RNA into a flexible elution volume ranging from 50 μL to 100 μL.
• Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary separation matrix.

Norgen’s EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Kit constitutes an all-in-one system for the purification of exosomes and the sequential isolation of RNA and free-circulating RNA from different plasma/serum sample volumes ranging from 50 μL and up to 10 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or any protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, NGS application, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Details

Supporting Data

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Kit Specifications (Spin Column)
Minimum Plasma Input	4 mL
Maximum Plasma Input	10 mL
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	40-45 minutes
Average Yields	Variable depending on specimen

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

K-GLULQR

SKU: 700007404

50 assays (manual) / 500 assays (auto-analyzer)

Content:	50 assays (manual) / 500 assays (auto-analyzer)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels

Analyte:	D-Glucose
Assay Format:	Spectrophotometer, Auto-analyzer
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	1 to 150 µg of D-glucose per assay
Limit of Detection:	2 mg/L
Limit of Quantification:	6 mg/L
Reproducibility (%):	< 5%
Reaction Time (min):	~ 5 min

The D-Glucose (Liquid Ready™) assay kit is a rapid (~ 5 min), method for the specific measurement and analysis of D-Glucose in wine, beverages, foodstuffs and other materials. Supplied as a 2-reagent format “ready to use” liquid stable formulation that is suitable for manual and auto-analyzer formats with a simple, reliable and accurate method.

Browse more of our monosaccharide and oligosaccharide assay kits.

Advantages

• “Ready to use” liquid stable formulation – no reconstitution needed
• Rapid reaction (~ 5 min)
• PVP incorporated to prevent tannin inhibition
• Standard included
• Suitable for manual and auto-analyzer formats
• Quick Reference Guide available
• Mega-Calc™ software tool is available for hassle-free raw data processing 

The D-Glucose (Liquid Ready™) assay kit is a rapid (~ 5 min), method for the specific measurement and analysis of D-Glucose in wine, beverages, foodstuffs and other materials. Supplied as a 2-reagent format “ready to use” liquid stable formulation that is suitable for manual and auto-analyzer formats with a simple, reliable and accurate method.

Overview

• Detection kits for Malaria
• Available in TaqMan format for analysis

Malaria is a mosquito-borne infectious disease and considered one of the leading causes of death in the world. Malaria is a fatal tropical disease that is caused by a parasite knows as Plasmodium. It is usually spread through the bite of an infected female mosquito. 300-500 million new infected cases are estimated every year with 1.5-2.7 million deaths worldwide. Most of the malaria-related deaths occur in sub-Saharan Africa. . Although blood is the sample most frequently used to make a diagnosis, both saliva and urine have been investigated as alternative, less invasive specimens

Malaria TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Malaria TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM34850 (100 preps)	Cat. TM34810 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
Malaria Primer & Probe Mix	280 μL	280 μL
Malaria Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1