PreviousNext[DM3260] FluoroBand™ 1 KB Plus (0.1-10 kb) Fluorescent DNA Ladder, 500 μlPreviousNext
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The DM3260 FluoroBand™ 1 KB Plus (0.1-10 kb) Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM3260 is composed of 19 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200, and 100 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains three enhanced bands (3 kb, 1.5 kb and 500 bp) for easier reference. In addition, three tracking dyes, Xylene cyanol FF, Bromophenol blue and Orange G which mimic the migration of 4,000 bp, 500 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.
Detail
Description
The DM3260 FluoroBand™ 1 KB Plus (0.1-10 kb) Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM3260 is composed of 19 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200, and 100 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains three enhanced bands (3 kb, 1.5 kb and 500 bp) for easier reference. In addition, three tracking dyes, Xylene cyanol FF, Bromophenol blue and Orange G which mimic the migration of 4,000 bp, 500 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.
Features
Sharp bands
Quick reference— enhanced bands
Ready-to-use— premixed with loading dye for direct loading
Stable— room temperature storage over 6 months
Directly observed by UV or blue light— premixed with high sensitive DNA fluorescent dye
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
100 ~ 10,000 bp
Concentration
87 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Protected from light Room temperature for 6 months 4°C for 12 months -20°C for 24 months
Other Products
DBCO-PEG36-TFP ester
Product Info
Document
Product Info
DBCO-PEG36-TFP ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The hydrophilic PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules. The TFP ester is less susceptible to undergo hydrolysis compared to the NHS ester. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
DBCO-PEG36-TFP ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The hydrophilic PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules. The TFP ester is less susceptible to undergo hydrolysis compared to the NHS ester. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Simultaneously clean-up and normalize PCR products
Fast (less than 20 minutes), high-throughput and easy processing using centrifugation
Sufficient elution volume (100 µL) for repeat or future assays
Non-magnetic bead purification
Norgen’s NGS Normalization 96-Well Kit allows for high-throughput NGS library PCR amplicon clean-up and normalization of NGS library PCR product concentration. The clean-up removes all PCR by-products including primers, dimers, enzymes and unincorporated nucleotides during the library prep PCR step. Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. The amount of Norgen’s matrix in each 96 well is optimized to have a limited binding capacity, thus each well can elute an equal concentration of PCR product. The process involves first adding 3 volumes of Buffer SK to the PCR product, and the mixture is then loaded onto the 96-Well Normalization Plate. The bound PCR amplicon is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified PCR amplicon is eluted with Elution Buffer B. The purified and normalized library PCR amplicon can then be used in NGS workflows and other sequencing applications.
Storage Conditions All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
MagPure Circulating DNA Mini Kit is designed for purification of high quality circulating DNA (cfDNA) fromcell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
Details
Specifications
Features
Specifications
Main Functions
Isolation circulating DNA from 0.2-0.6ml plasma, serum, body fluids
Applications
qPCR, NGS, etc.
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Serum, plasma
Sample amount
0.2-0.6ml
Elution volume
≥30μl
Time per run
≤50 minutes
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.
Advantages
Economy – less than 50% of the price of Qiagen and other imported products
Automatic – without labour
Kit Contents
Contents
IVD5432
Purification Times
200
MagPure Particles G
14 ml
Carrier RNA
310 μg
Proteinase K
180 mg
Protease Dissolve Buffer
10 ml
Buffer MLK
250 ml
Buffer MAW1
250 ml
Buffer MW2*
2 x 50 ml
Elution Buffer
60 ml
Contents
IVD5432-F-96A
IVD5432-F-96B
IVD5432-F-96C
Sample amount
200～350μl
400~700μl
Carrier RNA
110 μg
110 μg
Proteinase K
50 mg
100 mg
Protease Dissolve Buffer
5 ml
6 ml
Elution Buffer
15 ml
15 ml
Tip
1
1
Sample Plate A
500μl Buffer MLK
500μl Buffer MLK
Sample Plate B
/
500μl Buffer MLK
Wash Plate 1
700μl Buffer MAW1
700μl Buffer MAW1
Wash Plate 2
25μl Buffer MPG2700μl Buffer MW2
25μl Buffer MPG2700μl Buffer MW2
Wash Plate 3
700μl Buffer MW2
700μl Buffer MW2
Elution Plate
/
/
Contents
IVD5432-TL-06A
IVD5432-TL-06B
IVD5432-TL-06C
Sample amount
300~350μl
600~700μl
900~1050μL
Carrier RNA
310 μg
310 μg
310 μg
Proteinase K
50 mg
100 mg
150 mg
Protease Dissolve Buffer
6 ml
6 ml
10 ml
Elution Buffer
15 ml
15 ml
15 ml
DA-Tip
12
12
12
Row 1/7
600μl Buffer MLK
600μl Buffer MLK
600μl Buffer MLK
Row 2/8
/
600μl Buffer MLK
600μl Buffer MLK
Row 3/9
600μl Buffer MAW1
600μl Buffer MAW1
600μl Buffer MLK
Row 4/10
20μl Buffer MPG2600μl Buffer MW2
20μl Buffer MPG2600μl Buffer MW2
30μl Buffer MPG2900μl Buffer MAW1
Row 5/11
600μl Buffer MW2
600μl Buffer MW2
900μl Buffer MW2
Row 6/12
/
/
/
Storage and Stability
Proteinase K, Carrier RNA and MagPure Particles G should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should beredissolved before use. Make sure that all buffers are at room temperature when used.
Document
MagPure Circulating DNA Mini Kit is designed for purification of high quality circulating DNA (cfDNA) fromcell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
