Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Nitrogen, Primary Amino Nitrogen, YAN
Assay Format:
Spectrophotometer, Microplate, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
0.2 to 10 µg of amino nitrogen per assay
Limit of Detection:
2.59 mg N/L
Reaction Time (min):
~ 15 min
Application examples:
Grape juice, must, wine and other materials.
Method recognition:
Novel method
The Primary Amino Nitrogen (PANOPA) Assay Kit is suitable for the measurement and analysis of primary amino nitrogen in grape juice/must and wine.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
All reagents stable for > 2 years after preparation
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual, microplate and auto-analyser formats
Other Products
High-end thermoshaker for microplates & tubes
Product Info
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Product Info
Compact and stylish designed, the BioShake iQ high-end thermoshaker lets you perform all your standard mixing runs with a minimum of adjustments. The BioShake iQ offers outstanding performance to handle a wide range of applications across biotechnology, pharmaceutical and academic research.
Small. Light. Efficient. Quiet.
For microplates, tubes and glass vials
Fast & high-precision heating
Temperature range: ambient to 99°C
Precise mixing from 200 – 3,000 rpm
Wide range of thermo adapters
Stylish aluminium housing
ADVANCED TECHNOLOGY
Save time.
By combining the mixing operation with the incubation phase, reaction process times and operator workload are reduced and efficiency of many procedures is increased, resulting in a higher throughput.
The first-class finished aluminium housing gives the BioShake iQ its essential functionality. It provides a high amount of security, device stability and ensures a long service life.
GENTLE MIXING OF SAMPLES
By planar orbital motion
German designed and manufactured, BioShake iQ thermoshaker offer an ultra-efficient, 2-dimensional shaking axis so that samples mix completely in a fraction of the time of competing systems.
The mixing orbit of 2.0 mm is always constant. The orbital shaking is precisely controlled, in fact, that you need never spin down your plates after mixing. Even tubes, vials, high density plates, or low sample volumes, offer no obstacle for these precision tools.
Fully adjustable between 200 rpm and 3,000 rpm, well beyond the speeds of most other brands, guarantees fast, splatter-free, mixing for tubes, glass vials or across an entire 384-well microplate.
HOMOGENEOUS HEATING
Using large-area heating source in combination with digital sensor electronic
The BioShake iQ heats from ambient to 99°C in a short time, provides ease of use features, amazing comfort and maximum safety.
Within the BioShake iQ thinnest large-area heating elements and sensors are integrated. During the heat up time the special electronic control system guarantees the same temperature for all vessels with minimal deviations. Temperatures are selectable from ambient temperature to 99°C in steps of 1.0°C. The nominal regulation accuracy is better than ±0.1°C with a uniformity of temperature distribution better than ±0.5°C at 45°C across the heating surface.
Exchangeable thermo adapter plates and thermal blocks enable a perfect adaptation to microplates, tubes or glass vials.
UNIVERSAL SPRING CLAMPS
Suitable for all microplates
In the corners eight spring clamps are inserted for easy fixation of skirted microplates anddeep well plates in SBS format 127.7 x 85.5 mm without an attached adapter. These spring clamps lock the microplate quickly and safely, even at the highest mixing frequencies.
For flexible and deformable PCR plates we recommend using of the related PCR adapter.
Fast mixing processes with frequent plate changes can be performed in the routine as easy and safe.
COMFORT
Simple intuitive operation and programming
The BioShake iQ is operated from the front face of the enclosure which includes touch buttons and a display.
Special buttons for start, stop and storage of time and mixing modes enable the instrument to run complex applications. This opens new points of view on the daily laboratory work and optimizes routine application enormously.
The short mix button allows short and fast mixing in between.
The two line LCD display guarantees simultaneous and safe reading of all programmed and measured parameters as time, mixing frequency and temperature.
EXCHANGABLE THERMO ADAPTERS
For a wide range of applications
The BioShake iQ comes with a variety of standardized and specific adapter plates. The replacement of the adapters are very simple.
Perfect shaped adapters allow an optimal fit for standard tubes, lysis tubes, microplates, glass vials and other sample vessels. An excellent temperature uniformity and homogeneity is guaranteed for all samples.
UNIQUE DESIGN MEETS HIGH-END TECHNOLOGY
First-class finished aluminium housing
Unique and efficient design combined with the most compact housing result in beautiful and well-defined laboratory equipment. Therefore, the first-class finished aluminium housing gives the BioShake iQ its essential functionality. It provides a high amount of security, device stability and ensures a long service life.
Safety of the user and ease of use are clearly a priority.
MADE IN GERMANY
High-end instruments for daily routine
A perfectly harmonious blend of high-tech and handmade. “Made in Germany” has always been a recipe for success for QInstruments. 100% of QInstruments production takes place in Germany.
The focus is on human diligence and on being environmentally friendly. The company upholds its quality guarantee by means of consistent quality management. For more than 15 years these lab automation professionals from Jena have used only high-quality materials to ensure sustainable production, applied innovative thinking and undertaken research in a future-oriented way – all as a matter of course.
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For microplates, tubes and glass vials Fast & high-precision heating Temperature range: ambient to 99°C Precise mixing from 200 – 3,000 rpm Wide range of thermo adapters Stylish aluminium housing
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Lactose
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
1 to 50 µg of lactose (or 0.50 to 25 µg of D-glucose)
Limit of Detection:
1.62 mg/L
Reaction Time (min):
~ 10 min
Method recognition:
AOAC Method 2020.08
The K-LOLAC test kit offers a rapid, novel, sequential measurement of free-glucose and lactose in conventional, low-lactose and lactose-free dairy products. This sequential assay format reduces the manual input required by an analyst when compared to traditional lactose assay formats and therefore improves both accuracy and efficiency. When used in combination with the Megazyme Creep Calculator provided, the β-galactosidase employed in this kit allows for the selective measurement of lactose in the presence of galacto-oligosaccharides (GOS) which are commonly found in lactose-free dairy products. This constitutes a significant improvement over existing commercially available lactose assay kits which typically overestimate lactose content in lactose-free samples due to the unselective hydrolysis of GOS by β-galactosidase. Lastly, the sensitivity of the K-LOLAC assay kit has been doubled through the use of a cascade biochemical pathway, helping to significantly reduce the LOD and LOQ for the assay.
Blood samples contain rich DNA, including mitochondrial DNA, genomic DNA, circulating DNA (mostly released into blood after tumor cell apoptosis) in white blood cells, as well as parasitic viral or microbial DNA. These DNA are important parameters in clinical testing or diagnosis, which are also valuable materials for medical research. There are three main issues with extracting DNA from blood samples:
1. The sample is highly infectious, posing great harm to operators and the environment.
2. The source of DNA is complex and aportion of the nucleic acid, such as viral DNA or free DNA, may be lost during the operation, leading to downstream detection failure;
3. Blood sample contains a large amount of impurities and inhibitory factors.
Currently there are many methods available for extracting DNA from whole blood samples, such as phenol chloroform extraction, salting out method, etc. However, these methods require pre-treatment of blood sample, which removes red blood cells and isolate white blood cells in the first step. Due to the requirement that it cannot inactivate or kill pathogens during the process of removing red blood cells, the waste liquid (red blood cell lysate) and consumables may be contaminated by pathogens and become infectious, posing a danger to the entire laboratory environment and operators. In addition, during the process of removing red blood cells, useful nucleic acid information such as viruses, microorganisms, or circulating DNA is also lost, leading to experiment or detection failures.
The HiPure Blood DNA Kits series provided by Magen Company uses silica gel column purification technology, which can directly lyse whole blood samples without the need for white blood cell separation. Whole blood samples are directly mixed with lysates and proteases, resulting in the inactivation of pathogens, greatly reducing the infectivity, environmental pollution, and the chance of operators being infected. Due to the direct lysis and digestion of samples, except lymphocyte DNA, other circulating DNA as well as DNA from viruses and microorganisms, can also be recovered.
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from whole blood, tissue and culture cells.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 10ml blood and 1g tissue using Maxi column
Applications
PCR, southern bolt and virus detection, etc
Purification method
Maxi spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Tissue, cell, blood, saliva, swab, blood spot, semen and other clinical samples
Sample amount
3-10ml
Elution volume
≥700μl
Time per run
≤90 minutes
Liquid carrying volume per column
4ml
Binding yield of column
5mg
Principle
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
Fast – without separation of leukocytes, organic extraction or ethanol precipitation
Simple – all nucleic acids can be obtained by direct digestion
Pertinence – specially designed for isolating DNA from 3-10ml blood and related body fluids
Wide applicability – handle a variety of liquid samples
Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
*Note：Leukocyte protocol can be used when large volume whole blood samples need to be processed. Whole blood was treated with red blood cell lysate, and white blood cells were obtained by centrifugation before extraction
Document
Blood samples contain rich DNA, including mitochondrial DNA, genomic DNA, circulating DNA (mostly released into blood after tumor cell apoptosis) in white blood cells, as well as parasitic viral or microbial DNA. These DNA are important parameters in clinical testing or diagnosis, which are also valuable materials for medical research. There are three main issues with extracting DNA from blood samples:
