Hydrolysis probe genotyping master mix for use with 5’ nuclease assays such as TaqMan™, BHQ®, BHQplus® & Zen™ probes. Accurate, robust performance at competitive prices.
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Hydrolysis probe genotyping master mix for use with 5’ nuclease assays such as TaqMan™, BHQ®, BHQplus® & Zen™ probes. Accurate, robust performance at competitive prices.
About
ProbeSure Master Mix combines class-leading performance with the most competitive prices in the market. ProbeSure Master Mix has provided users with accurate, robust, and consistent performance for many years. It has been carefully optimised to work over a wide range of reaction volumes and sample template qualities.
ProbeSure Master Mix is supplied at 2x concentration for convenience and is supplied with the ROX normalising dye at either high level (500 nM final concentration), low level (25 nM final concentration) or without ROX.
Other Products
Cod UNG
Product Info
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Product Info
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Cod Uracil-DNA Glycosylase (Cod UNG) from Atlantic Cod is the only commercially available UNG enzyme that is completely and irreversibly inactivated by moderate heat treatment. The enzyme is produced in a recombinant E. coli (ung-) strain that contains a modified Cod UNG gene.
Key Features:
Heat-labile – Completely and irreversibly inactivated at 55°C
Contamination control – ideal in applications below
Use of Cod UNG makes contamination control possible in RT-PCR
Does not degrade PCR product post-PCR. This makes downstream use of the PCR product possible
High purity enzyme, tested free of contaminating nucleases
There are several commercially available Uracil-DNA glycosylases on the market today. Most of them are of bacterial origin and work well if you have no intention to further analyze the PCR products post-PCR. However, if you want to store your PCR products for downstream analysis such as cloning and sequencing, the reactivation of UNG and subsequent degradation of your PCR products are a problem with most of the commercially available UNGs. Cod UNG from ArcticZymes is completely and irreversibly inactivated by heat thus ensuring that sample integrity is maintained long-term regardless of storage conditions.
This is illustrated in figure 1, below
Figures
Properties
Recommended Protocols
1. Contamination control in PCR, qPCR and one-step RT-qPCR
Cod UNG works in all commercially available master mixes.
Be sure that you have used dUTP containing dNTP mixes in your previous PCR experiments.
Add 0.2 U Cod UNG directly to your 20 µl PCR reaction.
pre-incubate for 5 min at room temperature.
For RT-qPCR, reverse transcribe your RNA at 50-55°C.
Run your PCR.
Store your PCR product at -20°C or 4°C degrees.
2. Contamination control in RT-LAMP
Cod UNG is ideal for contamination control in RT-LAMP. One unit of Cod UNG per 30 μl reaction is sufficient for removing even high concentrations of carry-over contamination.
Ensure that you use dNTP mixes containing dUTP in your experiments.
Check that the RT-LAMP reaction is compatible with dUTP by running side-by-side reactions containing different ratios of dUTP to dUTP (100% dUTP, 90% dUTP, 80% dUTP and 0% dUTP).
Add 1 U Cod UNG directly to your 30 µl RT-LAMP reaction.
Prepare the reaction mix on ice.
Analyze your RNA at 65°C, no preincubation is necessary.
Cod Uracil-DNA Glycosylase (Cod UNG) from Atlantic Cod is the only commercially available UNG enzyme that is completely and irreversibly inactivated by moderate heat treatment. The enzyme is produced in a recombinant E. coli (ung-) strain that contains a modified Cod UNG gene.
Short term stability: 2-8oC, Long term stability: See individual component labels
Analyte:
D-Fructose, D-Glucose
Assay Format:
Spectrophotometer, Auto-analyzer
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
2.5 to 150 µg of D-glucose plus D-fructose per assay
Limit of Detection:
6 mg/L
Limit of Quantification:
16 mg/L
Reproducibility (%):
< 5%
Reaction Time (min):
~ 15 min for Total Sugars
The D-Fructose/D-Glucose (Liquid Ready™) assay kit is a method for the specific measurement and analysis of D-Fructose/D-Glucose in wine, beverages, foodstuffs and other materials. Measurements can be performed separately or combined. Supplied as a 2-reagent format “ready to use” liquid stable formulation that is suitable for manual and auto-analyzer formats with a simple, reliable and accurate method.
”Ready to use” liquid stable formulation – no reconstitution needed
PVP incorporated to prevent tannin inhibition
Standard included
Suitable for manual and auto-analyzer formats
Quick Reference Guide available
Mega-Calc™ software tool is available for hassle-free raw data processing
Document
The D-Fructose/D-Glucose (Liquid Ready™) assay kit is a method for the specific measurement and analysis of D-Fructose/D-Glucose in wine, beverages, foodstuffs and other materials. Measurements can be performed separately or combined. Supplied as a 2-reagent format “ready to use” liquid stable formulation that is suitable for manual and auto-analyzer formats with a simple, reliable and accurate method.
