The series of DNA Size Selection Kits (Magnetic Beads) were developed for DNA size selection using magnetic beads. A total of 11 kits are available, with different selection ranges spanning from 50 bp to over 10 kb. The kits provide a simple and quick approach for the enrichment of a specific range of DNA fragments. The kit workflow allows double-sided or single-sided size selection for specific size cutoffs.

Gel images of different ranges of size selection. Sheared human genomic DNA was used as input.

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DNA size selection is a selective capture of DNA fragments of a specific range of size for next-generation sequencing (NGS) library preparations, PCR, ChIP assay, DNA ligations, endonuclease digestions, adapter removal, and other genomics and molecular biology applications. DNA size selection is preferred after NGS library prep in most of the cases. The NGS library preparation is related to the quality of the sequencing data. Precise NGS library size selection can increase sequencing efficiency, improve data quality, and reduce costs.

There are two types of sequencing technologies: short-read sequencing and long-read sequencing. Short-read sequencing uses DNA libraries that contain small insert DNA fragments of similar sizes, usually several hundred base pairs. The sequencing efficiency can be improved if the DNA size selection is in the right range. Cat.# 20104S and 20104L are the best kits for NGS library size selection of illumina paired-end 100 (PE100) sequencing with 100-200 bp library inserts; Cat.# 20105S and 20105L are the best kits for NGS library size selection of illumina paired-end 150 (PE150) sequencing with 150-300 bp library inserts; and Cat.# 20106S and 20106L are the best kits for NGS library size selection of illumina paired-end 300 (PE300) sequencing with 300-600 bp library inserts.

Long-read sequencing uses a large DNA fragment as input and makes very long reads. Usually, library size selection is preferred to remove smaller fragments. Cat.# 20110S and 20110L are the best kits for long-read sequencing size selection with DNA sizes >5 kb, and Cat.# 20111S and 20111L are the best kits for long-read sequencing size selection with DNA sizes >10 kb.

The magnetic beads technology uses paramagnetic particles, also known as SPRI (Solid Phase Reversible Immobilization) beads, to bind DNA reversibly and selectively. DNA fragments can be size-selected and purified by changing the properties of the magnetic beads or SPRI beads. The magnetic beads can easily separate the beads-binding DNA from the contaminants and unwanted components in the samples. The samples after DNA size selection are free of contaminants such as buffer components, enzymes, proteins, salts, dNTPs, primers, and adapters. Our proprietary magnetic beads reagents improve yield, selectivity, and reproducibility.

Specific DNA fragments at a certain length range can be purified simply using magnetic separation with different beads components, avoiding tedious and time-consuming gel extraction and column-based purification. The magnetic beads method is popular for common DNA size selection, including library size selection. The first beads-binding step, referred to as the right-side clean-up, removes large DNA fragments. The large DNA fragments are bound to the beads and are discarded. The desired DNA fragments in the supernatant are transferred to a new well, and new beads are added to the supernatant for the second beads-binding, referred to as the left-side clean-up. The double-size selected DNA fragments are eluted after ethanol rinsing.

DNA size selection with dual clean-ups.

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A single clean-up is needed for DNA size selection with large fragments. In this case, only the large DNA fragments are bound to the beads. The selected larger DNA fragments are eluted after ethanol rinsing.

DNA size selection with single clean-up for >5 kb and >10 kb DNA.

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Features of DNA size selection and library size selection

• • • High specificity and high recovery of size selection
    • 11 selection ranges are available, including 5 ranges for NGS library size selection
      • • 50-100 bp
        • 100-200 bp
        • 200-500 bp
        • 250-350 bp: ideal for illumina PE100 sequencing
        • 300-450 bp: ideal for illumina PE150 sequencing
        • 450-750 bp: ideal for illumina PE300 sequencing
        • 500-1000 bp
        • 1-3 kb
        • 1-5 kb
        • >5 kb: ideal for long-read sequencing
        • >10 kb: ideal for long-read sequencing
    • Fast and simple
      • • 20-min protocol
        • No gel purification required
        • No columns required
        • No centrifugation required
    • Efficient removal of contaminants and unwanted components

Overview

• Can be used for the clean up of both RNA and DNA from enzymatic reactions, labeling etc.
• Purifies all sizes of RNA, from large mRNA down to microRNA (miRNA)
• Purifies all sizes of DNA, from small PCR products to plasmids to genomic DNA
• Removes endotoxins for transfection of injection ready RNA or DNA
• Rapid and efficient spin-column format (20 minutes)
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s CleanAll DNA/RNA Clean-Up and Concentration Micro Kit provides a rapid method for the purification, cleanup and concentration of RNA or DNA from different isolation methods or upstream applications.   The kit can be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions. 

The CleanAll Kit purifies RNA from phenol/guanidine-based protocols or from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. Furthermore, endotoxins can be removed from previously purified RNA solutions.

The kit can be used to clean up DNA from digestions, ligations, PCR reactions, labeling reactions, DNA modification reactions and staining. The kit also provides a protocol for the rapid removal of endotoxins from previously purified DNA down to 0.1 EU/µg DNA or less.

Purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA).

Purify all sizes of DNA, from small PCR products, native or linearized plasmids, to genomic DNA. Using an alternative protocol, even oligonucleotides and smaller DNA fragments can be purified.

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Supporting Data

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Kit Specifications
Column Binding Capacity	50 μg RNA 10 μg DNA
Maximum Column Loading Volume	600 μL
Minimum Elution Volume	20 μL
Time to Complete 10 Purifications	20 minutes
Size of RNA Purified	All sizes, including small RNA (<200)
Size of DNA Purified	≥ 100 bp*
Average Recovery	≥90% for RNA ≥90% for DNA 100 bp to 10 kbp ≥75% for DNA ≥ 10 kbp

* Applicable to smaller fragments or oligonucleotides with alternative protocol

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.

Component	Cat. 23800 (50 preps)
Binding Buffer H	60 mL
Wash Solution K	27 mL
Elution Buffer L	15 mL
Micro Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Introduction

MagPure Circulating DNA Mini Kit is designed for purification of high quality circulating DNA (cfDNA) fromcell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.

Details

Specifications

Features	Specifications
Main Functions	Isolation circulating DNA from 0.2-0.6ml plasma, serum, body fluids
Applications	qPCR, NGS, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Serum, plasma
Sample amount	0.2-0.6ml
Elution volume	≥30μl
Time per run	≤50 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.

Advantages

• Economy – less than 50% of the price of Qiagen and other imported products
• Automatic – without labour

Kit Contents

Contents	IVD5432
Purification Times	200
MagPure Particles G	14 ml
Carrier RNA	310 μg
Proteinase K	180 mg
Protease Dissolve Buffer	10 ml
Buffer MLK	250 ml
Buffer MAW1	250 ml
Buffer MW2*	2 x 50 ml
Elution Buffer	60 ml

Contents	IVD5432-F-96A	IVD5432-F-96B	IVD5432-F-96C
Sample amount	200～350μl	400~700μl
Carrier RNA	110 μg	110 μg
Proteinase K	50 mg	100 mg
Protease Dissolve Buffer	5 ml	6 ml
Elution Buffer	15 ml	15 ml
Tip	1	1
Sample Plate A	500μl Buffer MLK	500μl Buffer MLK
Sample Plate B	/	500μl Buffer MLK
Wash Plate 1	700μl Buffer MAW1	700μl Buffer MAW1
Wash Plate 2	25μl Buffer MPG2700μl Buffer MW2	25μl Buffer MPG2700μl Buffer MW2
Wash Plate 3	700μl Buffer MW2	700μl Buffer MW2
Elution Plate	/	/

Contents	IVD5432-TL-06A	IVD5432-TL-06B	IVD5432-TL-06C
Sample amount	300~350μl	600~700μl	900~1050μL
Carrier RNA	310 μg	310 μg	310 μg
Proteinase K	50 mg	100 mg	150 mg
Protease Dissolve Buffer	6 ml	6 ml	10 ml
Elution Buffer	15 ml	15 ml	15 ml
DA-Tip	12	12	12
Row 1/7	600μl Buffer MLK	600μl Buffer MLK	600μl Buffer MLK
Row 2/8	/	600μl Buffer MLK	600μl Buffer MLK
Row 3/9	600μl Buffer MAW1	600μl Buffer MAW1	600μl Buffer MLK
Row 4/10	20μl Buffer MPG2600μl Buffer MW2	20μl Buffer MPG2600μl Buffer MW2	30μl Buffer MPG2900μl Buffer MAW1
Row 5/11	600μl Buffer MW2	600μl Buffer MW2	900μl Buffer MW2
Row 6/12	/	/	/

Storage and Stability

Proteinase K, Carrier RNA and MagPure Particles G should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should beredissolved before use. Make sure that all buffers are at room temperature when used.

MagPure Circulating DNA Mini Kit is designed for purification of high quality circulating DNA (cfDNA) fromcell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.

Details