ProbeSure OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single step. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA. ProbeSure OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single step. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA. ProbeSure OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single step. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA. ProbeSure OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single step. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA.
Detail
ProbeSure OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single step. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA.
ProbeSure OneStep RT-PCR Master Mix is highly efficient and sensitive, enabling detection of low-abundance RNA targets, particularly useful for applications such as gene expression analysis and viral RNA detection. ProbeSure OneStep RT-PCR Master Mix demonstrates robust performance across a wide range of RNA templates and can be employed for both routine and challenging samples.
Other Products
OT-2 HEPA Module
Product Info
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Product Info
The Opentrons HEPA Module enables you to run sensitive contamination-prone applications. It removes 99.99% of 0.3 μm DNA-containing particulates and biological contaminants like bacteria, fungi, and other microorganisms from the air, creating a clean work environment inside the OT-2.
The Opentrons HEPA Module comes in two different voltages. Your account manager will help you choose the correct one for your geographic region.
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The Opentrons HEPA Module enables you to run sensitive contamination-prone applications. It removes 99.99% of 0.3 μm DNA-containing particulates and biological contaminants like bacteria, fungi, and other microorganisms from the air, creating a clean work environment inside the OT-2.
The Opentrons HEPA Module comes in two different voltages. Your account manager will help you choose the correct one for your geographic region.
Protease K is a serine protease with high enzymatic activity and wide substrate specificity. It can preferentially decompose ester and peptide bonds adjacent to the ends of hydrophobic amino acids, sulfur-containing amino acids and aromatic amino acids C, and is often used to degrade proteins to produce short peptides. It has the typical catalytic triad Asp39-His69-Ser224 characteristic of serine proteases and two Ca2+ binding sites around the active center increase its stability, allowing it to maintain high enzyme activity under a wider range of conditions.
Technical index
Appearance: Colorless to light brown liquid; Specific activity: ≥800 U/mL; Protein concentration: ≥20 mg/mL
Enzymatic property
Source: Tritirachiumalbum; Category: EC 3.4.21.64; Molecular weight: 29 kDa(SDS-PAGE); Isoelectric point: 7.81; The optimal pH: 7.0-12.0 has high activity; Optimum temperature: 65℃; pH stability: pH 4.5-12.5(25℃,16 h); Thermal stability: stable below 50℃ (pH 8.0,30 min); Storage stability: 25℃ a year activity of more than 90% activator SDS, urea; Inhibitors: diisopropyl fluorophosphate (DFIP), benzoyl fluoride (PMSF); Storage conditions: 2-8℃, valid for 24 months.
use
Genetic diagnostic kit;
RNA and DNA extraction kit;
Extraction of non-protein components from tissues, degradation of protein impurities, such as DNA vaccine and heparin preparation;
Preparation of chromosome DNA by pulsed electrophoresis;
Western blot;
Research and development and mass production of enzymatic glycosylated albumin reagents for in vitro diagnosis.
Matters needing attention
Wear protective gloves and goggles when using, and keep well ventilated after use. This product may cause allergic skin reactions.
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Note: Price not include shipment & duty, contact us to get full quote.