ProbeSure OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single step. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA. ProbeSure OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single step. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA. ProbeSure OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single step. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA. ProbeSure OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single step. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA.
Detail
ProbeSure OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single step. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA.
ProbeSure OneStep RT-PCR Master Mix is highly efficient and sensitive, enabling detection of low-abundance RNA targets, particularly useful for applications such as gene expression analysis and viral RNA detection. ProbeSure OneStep RT-PCR Master Mix demonstrates robust performance across a wide range of RNA templates and can be employed for both routine and challenging samples.
Other Products
D6310C MagPure Fast Blood DNA Kit
Product Info
Document
Product Info
Introduction
This product provide fast and easy methods for purification of total DNA from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells. There is no need to use toxic phenol chloroform extraction or time-consuming alcohol precipitation. The extraction process finish in 40 minutes. Purified DNA includes genomic DNA, mitochondrial DNA, viral DNA (e.g. HBV), or DNA from other parasitic microorganisms. The obtained DNA can be directly used in PCR, viral DNA detection and other experiments.
This kit can use on manual protocol or 16/32/48 channel automated extraction system. No need to pause for the addition of binding solution (not applicable to 96/192 instruments)
Details
Kit Contents
Contents
D631001C
D631002C
D631003C
Purification Times
48
96
480
MagPure Particles
1.7 ml
3.5 ml
16 ml
Proteinase K
24 mg
50 mg
220 mg
Protease Dissolve Buffer
1.8 ml
3 ml
15 ml
Buffer MLA
30 ml
70 ml
300 ml
Buffer DW1
30 ml
70 ml
300 ml
Buffer EW
60 ml
120 ml
2 x 300 ml
Buffer MWX1
30 ml
70 ml
300 ml
Elution Buffer
10 ml
20 ml
60 ml
Prefilled Kit Contents
Product
Contents and volume
D6310-TL-06C
D6310-S-48
Proteinase K
50 mg
24 mg
Protease Dissolve Buffer
3 ml
1.8 ml
TL-Tip
12 pcs
24 pcs
Tip bottom plate/ Tip base reagent strip
Row 1/7：600μl Buffer MLA
6 plates
48 strips
Row 2/8：600μl Buffer MWX1
Row 3/9：600μl Buffer DW1
Row 4/10：30μl MagPure Particles 600μl Buffer EW
Row 5/11：600μl Buffer EW
Row 6/12：100μl Elution Buffer
Storage and Stability
Proteinase K Powder should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these condition.
Document
This product provide fast and easy methods for purification of total DNA from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells. There is no need to use toxic phenol chloroform extraction or time-consuming alcohol precipitation. The extraction process finish in 40 minutes. Purified DNA includes genomic DNA, mitochondrial DNA, viral DNA (e.g. HBV), or DNA from other parasitic microorganisms. The obtained DNA can be directly used in PCR, viral DNA detection and other experiments.
Grape juice, wine must, wine and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Improved method
The L-Arginine/Urea/Ammonia test kit is specific and a rapid measurement and analysis of L-arginine, urea and ammonia in grape juice/must and wine.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
CE-IVD marked version available for in vitro diagnostic use
Available in TaqMan format for analysis
The Zika Virus (ZIKV) is an emerging mosquito-borne virus that was first identified in Uganda in 1947 in Rhesus monkeys through monitoring of sylvatic yellow fever. During large outbreaks in French Polynesia and Brazil, national health authorities reported potential neurological and auto-immune complications of ZIKV disease. Agencies investigating the Zika outbreaks are finding an increasing body of evidence about the link between ZIKV and microcephaly. Infection with ZIKV may be suspected based on symptoms and recent history (e.g. residence or travel to an area where ZIKV is known to be present). Zika virus diagnosis can only be confirmed by laboratory testing for the presence of ZIKV RNA in the blood or other body fluids, such as urine or saliva.
ZIKV TaqMan RT-PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
ZIKV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
