Propane-1,3-(PEG12-DBCO),2-alcohol is a PEG linker containing two DBCO moieties and a terminal primary hydroxyl group. The hydroxyl can react with a variety of functional groups and the hydrophilic PEG spacer arm can provide better solubility to labeled molecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research use only.
Propane-1,3-(PEG12-DBCO),2-alcohol is a PEG linker containing two DBCO moieties and a terminal primary hydroxyl group. The hydroxyl can react with a variety of functional groups and the hydrophilic PEG spacer arm can provide better solubility to labeled molecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research use only.
This product provides fast and easy method for purification of high purity DNA from bone samples. The obtained DNA can be directly used for PCR and STR detection down stream application.
| Contents | D312402 | D312403 |
| Purification Times | 50 | 250 |
| HiPure DNA Mini Columns I | 50 | 250 |
| 2ml Collection Tubes | 50 | 250 |
| Buffer BGL | 40 ml | 180 ml |
| Buffer GXP | 30 ml | 150 ml |
| Buffer GW1* | 13 ml | 53 ml |
| Buffer GW2* | 20 ml | 2 x 50 ml |
| DTT Powder | 235 mg | 2 x 235 mg |
| Proteinase K | 48 mg | 240 mg |
| Protease Dissolve Buffer | 5 ml | 15 ml |
| Elution Buffer | 15 ml | 60 ml |
Storage and Stability
This product can be stored at room temperature (15~25°C) for 18 months. Proteinase K/DTT dry powder can be transported and stored at room temperature. For long-term storage (>6 months), it is recommended to store at -20~8°C. Dissolved Proteinase K should be stored at -20~8°C. The dissolved DTT should be stored at -20°C.
This product provides fast and easy method for purification of high purity DNA from bone samples. The obtained DNA can be directly used for PCR and STR detection down stream application.
PACE (PCR Allelic Competitive Extension) genotyping chemistry is a homogeneous, PCR-based allele-specific technology for the analysis of DNA sequence variants, most commonly SNPs (Single Nucleotide Polymorphisms) and Indels (insertion / deletions).
PACE genotyping chemistry is comprised of two parts:
When combined with sample DNA, these components create a PACE Genotyping Reaction, as illustrated in the figure below.
We have extensive knowledge and experience in assay design, especially when it comes to allele-specific PCR. PACE Genotyping Assays are available to purchase either Validated and Unvalidated. Validated assays require customer DNA to validate and optimise, for guaranteed performance. Unvalidated assays are designed in silico and supplied untested.
Safety Features
1. User-Friendly Design: The chamber lid and tabletop are covered with heat-insulating resin materials, providing both protection against burns and an aesthetically pleasing appearance. The upward-opening flip-top door saves space, while the rounded corners prevent injury. The specially designed steam discharge and collection system minimizes steam burn risks and enhances operational convenience.
2. Interlock Mechanism and Safety Protection: The safety interlock system is designed to be more sensitive and secure. The chamber lid cannot be opened if there is pressure or steam inside the chamber. The lid can only be opened when the pressure gauge reads “0” MPa. The sterilizer will not start heating if the chamber lid is not properly locked. These features enhance the overall safety of the machine.
3. Over-temperature and Heating Protection: The system continuously monitors the temperature inside the sterilization chamber. If the temperature becomes too high or heating is abnormal, power will be automatically cut off to prevent overheating.
4. Automatic Fault Detection System: The machine performs an automatic self-check when powered on. If a fault occurs during operation, an audible alarm will sound, and a fault code will be displayed.
5. Lid Closure Check System: The system automatically detects whether the chamber lid is securely locked. If the lid is not properly locked, the sterilizer will not start.
6. Leakage, Over-current, and Short-Circuit Protection: If there is leakage in the heating elements or other components, or if over-current or short-circuit conditions occur, the system will automatically cut off power to prevent user injury.
7. Water Shortage and Independent Dry-Burning Protection: Equipped with a low water level sensor and independent dry-burning protection device, the system can monitor the water level in the chamber in real-time to prevent dry burning and avoid false readings. If the low water level sensor fails, the overheat and dry-burning protection system will automatically activate, cut off power to the heating ring, and sound an alarm to ensure safety.
8. Dual Pressure Protection: The system includes both a mechanical safety valve and an electronic over-pressure protection system. If pressure becomes abnormal, the system will relieve pressure and cut off power while sounding an alarm.
9. Back end Safety Testing: The system can be periodically tested for safety and protective measures.
10. Over-pressure Testing: Regular testing ensures that the instrument’s over-pressure protection system can automatically cut off power or that the safety valve can open to relieve pressure.
11. Performance Testing: The instrument’s safety performance has been tested by national authorized inspection agencies, and a test report is available.
Technical Parameters
Model | HB65A Standard TypeHB65B Auto Water Replenishment TypeHB65C Auto Drying Type | HB90AStandard TypeHB90BAuto Water Replenishment TypeHB90CAuto Drying Type | HB110AStandard TypeHB110BAuto Water Replenishment TypeHB110CAuto Drying Type | |
| Capacity | 65L | 90L | 110L | |
| Dimensions (L × W × H) mm | 664×660×994.5 | 664×660×994.5 | 664×660×1200 | |
| Sterilization Chamber Dimensions (Diameter × Height) mm | φ400×530 | φ400×730 | φ400×920 | |
| Rated Power | 2800W | 3400W | 4500W | |
| Rated Voltage | 220V±10%50Hz | |||
| Temperature Display Accuracy | 0.1℃ | |||
| Operating Environment | 5℃~40℃、Relative Humidity10%～85% | |||
| Sterilization Chamber Material | SUS304Stainless Steel | |||
| Sterilization Working Temperature | 105℃~143℃ | |||
| Sterilization Time Pre-set Range | 1minute ～9999minutes | |||
| Melting Temperature Pre-set Range | 60℃~100℃ | |||
| Melting Time Pre-set Range | 1minute～9999minutes | |||
| Insulation Temperature Pre-set Range | 45℃~60℃ | |||
| Insulation Time Pre-set Range | 1minute～9999minutes | |||
| Door Opening Temperature Pre-set Range | Solid, Agar, and Waste Modes: 40°C ~ 99°C Liquid Mode: 40°C ~ 80°C | |||
| Clock FunctionTimer Pre-set Range | Timer Pre-set Range: Set operating time (year, month, day, hour, minute), check current time (year, month, day, hour, minute), total sterilization cycles, fault occurrence time, and code. | |||
| Pressure Gauge Display Range | 0～0.6Mpa | |||
| Pressure | Chamber Design Pressure: 0.35 MPa; Each product comes with a quality certificate and a certificate issued by the special equipment manufacturing supervision and inspection department. | |||
| Pressure Safety Protection | Dual independent pipeline measurement and control, mechanical and electronic dual pressure protection | |||
Sterilization Modes | · Solid Mode: Heating → Sterilization → Exhaust, Air Cooling· Solid with Drying Mode: Heating → Sterilization → Drainage, Exhaust → Drying· Liquid Mode: Heating → Sterilization → Pre-cooling → Exhaust· Liquid with Insulation Mode: Heating → Sterilization → Pre-cooling → Exhaust → Insulation· Waste Mode: Heating → Sterilization → Exhaust, Air Cooling· Agar Mode: Heating → Melting → Insulation | |||
| Exhaust Control and Water Tank | Fully automatic internal exhaust (see instructions for exhaust temperature settings), built-in large-capacity steam condensate collection bottle and water tank | |||
| Cover Opening Method and Interlocking Device | Unique eight-column interlocking device, flip-cover switch, touch-type handle | |||
| Controller and Memory Storage Function | Microcomputer intelligent control system. 6 fixed operation modes, 82 modifiable modes, 88 memory storage programs | |||
| Safety Devices | Door lock interlocking device; Cover closing check; Dry burn protection; Water level sensor monitoring; Electronic over pressure protection; Mechanical safety valve; Over temperature and heating protection; Over current and short circuit protection; Leakage protection; Cooling lock; Burn injury safety protection; Automatic fault detection system | |||
| Accessories | Condensate collection bottle, water separation plate, drainage hose, stainless steel basket | |||
| Optional Accessories | Printer, cooling fan, item temperature sensor, USB output | |||
| Stainless Steel Baskets | (φ360×230)1pcs(φ360×200)1pcs | (φ360×200)1pcs(φ360×400)1pcs | (φ360×200)2pcs(φ360×400)1pcs | |
Note: The upper limits for cooling lock door temperature and exhaust temperature are related to the boiling point.
Other Optional Accessories
1. Dot Matrix Printer: Capable of printing temperature, time, F0 value, and pressure data. USB data export and curve plotting.
2. Stainless Steel Basket with Base.
3. Pressure Sensor
4. Stainless Steel Bucket
5. Item Thermometer
6. 3Q Validation Adapter
Features and functions:
1. Large-diameter sterilization chamber：The 40 cm large-diameter sterilization chamber provides an exceptionally spacious environment to meet a wide range of sterilization needs.
2. Sterilization temperature：105-138℃.
3. Exhaust control ：After sterilization is complete, the exhaust valve can automatically open to release the exhaust at a pre-set temperature.
4. Verification interface: Provides temperature and pressure inspection ports for easy calibration.
5. Saturated steam monitoring：The system automatically monitors the expulsion of cold air to ensure a pure steam sterilization environment, guaranteeing optimal sterilization effectiveness.
6. Pressure performance：The chamber is designed to withstand a pressure of up to 0.35 MPa, and each unit is tested with a water pressure of up to 0.44 MPa. Advanced pressure protection ensures the safety load of the equipment.
7. Intelligent control system:The microcomputer chip-based intelligent control system monitors the temperature and pressure inside the sterilization chamber in real-time. The chamber lid can only be opened under safe temperature and pressure conditions. The system also provides fully automatic control of the sterilization process and allows for convenient maintenance, inspection, calibration, recording, and future upgrades.
8. Six preset programs:Includes six fixed programs for liquid sterilization and holding, solid sterilization and drying, agar melting and holding, and waste sterilization.
9. Memory storage system:Users can set their own parameters, create multiple programs, and access them at any time. The system allows for operation permissions to be set at three levels—user, administrator, and engineer—to facilitate effective use and management of the sterilizer.
10. Door-opening temperature: Each program can be set with a specific cooling lock door-opening temperature based on the thermal inertia of the sterilized items. This prevents users from opening the door when the items (especially liquid sterilized items) are still in an unsafe condition, thus avoiding burns.
11. Drying function: Thorough drying that meets CE standards, with a cooling lock mechanism.
12. One-touch inquiry function: Allows checking of current time, total number of sterilization cycles, fault occurrence time, and codes.
13. Scheduled start function: Allows you to set a start time so that the sterilizer automatically starts at the scheduled time.
Model:
HB65/ HB90/ HB110
