Propargyl-PEG1-NHS ester is an amine reactive reagent that can be used for derivatizing peptides, antibodies, amine coated surfaces etc. The alkyne group reacts with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG1-NHS ester is an amine reactive reagent that can be used for derivatizing peptides, antibodies, amine coated surfaces etc. The alkyne group reacts with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Features:
1.Brushless frequency motor,in great torque, free maintenance, no powder pollution, quick in speed up and down.
2.Imported compressors fluorine free,double cycle cooling,cold and hot alternating easily, free environment pollution and precise in temperature control.
3. Microprocessor control, digital display which indicates the speed, time, RCF in operation, speed raising and reducing quickly, operate simply. Running parameter can be edited.
4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.
5. Automatically electric lid lock, super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable.
6. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction.
7. There are several kinds of rotors for your choice, adapters can be designed by experimental requirements.
8. 3 tiers protection steel cover and get the ideal centrifugation result.
TDL5M Technical Parameter：
| Max. Speed | 5000rpm |
| Max. RCF | 4730×g |
| Max. Capacity | 4×800ml( 4*450ml blood bags) |
| Time Range | 0~99h59min |
| RPM/RCF Convert | Yes |
| Noise (dB) | ≤ 65 |
| Temperature | -20℃-40℃ |
| Acc/Dec | 10 Kinds |
| Speed Accuracy | ±20r/min |
| Temperature Accuracy | ±1℃ |
| Voltage(V/Hz) | AC 220V/110V 50HZ/60HZ |
| Size (W x D x Hmm) | 700×715×420mm |
| Net Weight(Kg) | 125KG |
| Certificates | CE,ISO & Calibration report are available |
Matched Rotors for TDL5M
| Order No. | Rotor | Max Speed (rpm) | Max Volume(ml) | Max. RCF（×g） |
| NO30671 | Swing Rotor | 4000 | 4×800ml(Round Cup) | 3450 |
| NO30696 | Swing Rotor | 4000 | 4×500ml( Round Cup) | 3380 |
| NO30679 | Swing Rotor | 4000 | 4×500ml(Square Cup) | 3310 |
| NO31494 | Microplate Rotor | 4000 | 4×4×96 Well | 2840 |
| NO31491 | 4000 | 2×4×96 Well | 2490 | |
| NO31376 | 4000 | 2×3×48 Well | 2300 | |
| NO30690 | Swing Rotor | 4000 | 4×30×7ml | 3270 |
| 4×30×5ml | 2890 | |||
| 4×18×10ml | 3140 | |||
| NO30607 | Angle Rotor | 5000 | 12×15ml | 3080 |
| NO30611 | Angle Rotor | 5000 | 6×50ml | 2850 |
| NO30640 | Angle Rotor | 5000 | 4×50ml | 2520 |
| NO30613 | Angle Rotor | 5000 | 4×100ml | 2630 |
| NO30639 | Angle Rotor | 5000 | 24×15ml | 3500 |
| NO30641 | Angle Rotor | 5000 | 12×50ml | 3860 |
| NO30614 | Angle Rotor | 5000 | 6×100ml | 3130 |
| NO30642 | Angle Rotor | 4000 | 12×100ml | 2970 |
Adapter for 800ml and 500ml bucket
 VolumeCup | 5ml | 7ml | 10ml | 15ml | 50ml |
| 800ml（Round Cup） | 23pcs/cup | 23 | 19 | 14 | 5 |
| 500ml（Round Cup） | 18pcs/cup | 18 | 14 | 9 | 3 |
| 500ml（Square Cup） | 20pcs/cup | 20 | 16 | 12 | 5 |
Fig 1. Only three steps will be need for Sample Preparation and only 20 minitutes will be taken for Sample Preparation.
Fig 2. Seven concentration samples of 0.3fg/μL, 3fg/μL, 30fg/μL, 300fg/μL, 3pg/μL, 30pg/μL, 300pg/μL were detected. CV of each concentration was < 30%, Regression coefficient associated with standard solutions was 0.99992, and amplification efficiency was 100.370%.
Fig 3. Five concentration samples of 0.1fg/μL, 0.3fg/μL, 0.5fg/μL, 1fg/μL and 3fg/μL were detected, and 10 multiple wells were detected for each concentration. The CV of concentration values of samples with 0.3fg/μL and above concentrations were less than 30%.
Fig 4. DNA recovery can be determined by including samples spiked with known DNA amounts which are prepared from the corresponding DNA standards. Typically, the range for this value varies from 70% to 130%.
Fig 5. Only one Reagent for qPCR MIX.
Note: Price not include shipment & duty, contact us to get full quote.
The resDNASEQ CHO Residual DNA Quantitation kit is designed for the quantification of residual DNA from CHO, in cell lines which are used for production of biopharmaceutical products. The Ducky Bio residual DNA CHO Assay, based on proven real-time qPCR technology, makes testing of residual DNA from the Chinese hamster ovary (CHO) cell line rapid, specifc. The PCR-based assay is sensitive and specific for DNA from the CHO cell line and not subject to detection of human or environmental DNA that might be introduced during sample handling. The kit was developed to meet the sensitivity requirements defined by WHO (10 ng CHO DNA per therapeutic dose).
These kits allow for the isolation of DNA from the blood of various species, including humans and will recover genomic DNA, mitochondrial DNA, viral DNA and bacterial DNA. The purified DNA is of excellent quality and yield and completely compatible with any downstream application including PCR, qPCR and genotyping.
Blood DNA Isolation Mini Kit
Norgen’s Blood DNA Isolation Mini Kit is designed for the rapid preparation of DNA from up to 200 µL of whole blood using a rapid spin column protocol. Preparation time for a single sample is less than 30 minutes and each kit contains sufficient materials for 50 preparations.
Blood DNA Isolation Midi Kit
Norgen’s Blood DNA Isolation Kit is designed for the rapid spin column preparation of DNA from 0.3 to 2 mL volumes of whole blood. Preparation time for a single sample is less than 30 minutes, and each kit contains sufficient materials for 20 preparations.
Blood DNA Isolation Maxi Kit
This kit is designed for the rapid preparation of DNA from 3 mL up to 10 mL of whole blood. Preparation time for a single sample is less than 30 minutes.
Blood DNA Isolation 96-Well Kit (High Throughput)
This kit provides a rapid method for the high-throughput isolation of DNA from up to 200 µL of whole blood. Fast and easy processing using either a vacuum manifold or centrifugation.
Blood DNA Isolation Kit (Magnetic Bead System)
Norgen’s Blood DNA Isolation Kit (Magnetic Bead System) is designed for the rapid preparation of genomic DNA from up to 200 µL of whole blood from various species, including human. Purification is based on magnetic beads as the separation matrix. Norgen’s magnetic beads bind DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The genomic DNA is preferentially purified from other cellular proteinaceous components. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including real-time PCR, NGS and microarray analysis.
Blood DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
Figure 1 / 19
Click for expanded view
| Kit Specifications | |
| Maximum Blood Input | 200 µL |
| Average Yield (200 µL blood)* | 4-12 µg |
| Average Purity (OD260/280) | 1.8 – 1.9 |
| Time to Complete Purifications | 40 minutes (hands-on time Cat. 59800) 50 minutes (hands-on time Cat. 62600) |
* Average DNA yield will vary depending on the donor
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
| Component | Cat. 46300 (50 preps) | Cat. 46380 (100 preps) | Cat. 51400 (20 preps) | Cat. 31200 (12 preps) | Cat. 46350 (192 preps) | Cat. 59800 (50 preps) | Cat. 62600 (192 preps) |
|---|---|---|---|---|---|---|---|
| Lysis Buffer B | 20 mL | 2 x 20 mL | 2 x 40 mL | 2 x 110 mL | 2 x 40 mL | 20 mL | 1 x 40 mL 1 x 20 mL |
| Solution WN | 18 mL | 2 x 18 mL | 55 mL | 55 mL | 55 mL | 18 mL | 55 mL |
| Wash Solution A | 18 mL | 2 x 18 mL | 2 x 38 mL | 2 x 38 mL | 2 x 38 mL | – | – |
| Elution Buffer B | 30 mL | 2 x 30 mL | 30 mL | 30 mL | 2 x 30 mL | 15 mL | 1 x 30 mL 1 x 15 mL |
| Proteinase K in Storage Buffer | 1.2 mL | 2 x 1.2 mL | 4.4 mL | 8 mL | 4.5 mL | 1.2 mL | 4 mL |
| Magnetic Bead Suspension | – | – | – | – | – | 2 x 1.1 mL | 8.5 mL |
| Maxi Spin Columns | – | – | – | 12 | – | – | – |
| Mini Spin Columns | 50 | 100 | – | – | – | – | – |
| Midi Spin Columns | – | – | 20 | – | – | – | – |
| 96-Well Plate | – | – | – | – | 2 | – | 2 |
| Adhesive Tape | – | – | – | – | 4 | – | 2 |
| Collection Tubes | 50 | 100 | 20 | 12 | – | – | – |
| 96-Well Collection Plate | – | – | – | – | 2 | – | – |
| Elution Tubes | 50 | 100 | 20 | 12 | – | 50 | – |
| 96-Well Elution Plate | – | – | – | – | 2 | – | 2 |
| Product Insert | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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