Propargyl-PEG1-NHS ester

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Ten discrete fragments ranging from 300 bp to 5000 bp

The Norgen MidRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our MidRanger contains ten discrete fragments ranging from 300 bp to 5000 bp. This Ladder is ideal for sizing larger PCR products (>1kb) and most cloning applications.

Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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MidRanger 1kb DNA Ladder (Cat# 11700) – 100 loads

Ladder Properties:
• Ten discrete fragments ranging from 300 bp to 5000 bp

Fragment	Size (bp)	Mass (ng)
1	5000	88
2	4000	78
3	3000	67
4	2500	58
5	2000	50
6	1500	43
7	1000	25
8	700	34
9	500	33
10	300	24

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Cell Culture Dish 35mm

Cell Culture Dish provide Excellent surface smoothness gives clear microscopic observation

Specifications: 35mm. 60mm.100mm.150mm

PRODUCT FEATURES

• The product is made of medical grade USP CLASS VI polymer polystyrene
• The product is made under a 100,00- class dust-free manufacturing site
• Two kinds of product line up are providing.

For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.

For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.

• Gamma radiation sterilization
• Non-Pyrogenic, DNase/Rnase free.

Cell Culture Dish provide Excellent surface smoothness gives clear microscopic observation

Specifications: 35mm. 60mm.100mm.150mm

General information

Champion™ Competent Cells are chemically competent cells, which were prepared by SMOBIO to make E. coli perform excellent transformation efficiency. Standard transformation protocol is recommended for large plasmids or non-ampicillin selection. Time-saving transformation protocol is recommended for simple and rapid transformation. Champion™ Competent Cells are one of the fastest and simplest ready-to-use competent cell products in the world.

Kit contents

1. Champion™ Competent Cells
2. pUC19 Control Plasmid (5 μl, 10-4 μg/μl)
3. Champion™ Transformation Protocol Card

Shipping condition

Throughout the shipping process, the temperature is maintained under -70°C. 

Storage and expiration 

Champion™ Competent Cells must be stored between -70°C to -80°C. Subsequent freeze-thaw cycles will reduce transformation efficiency. If high efficiency is required for the experiment, do not use aliquots that have gone through several freeze-thaw cycles. The efficiency of Champion™ Competent Cells lasts for 1 year with proper storage.

Champion™ Competent Cells are chemically competent cells, which were prepared by SMOBIO to make E. coli perform excellent transformation efficiency. Standard transformation protocol is recommended for large plasmids or non-ampicillin selection. Time-saving transformation protocol is recommended for simple and rapid transformation. Champion™ Competent Cells are one of the fastest and simplest ready-to-use competent cell products in the world.